Background
Surgical site infections (SSIs) are postoperative infections encompassing the superficial, deep, and interstitial layers [
1‐
3]. SSI is a common nosocomial infection, leading to extended patient hospitalization and imposing substantial burdens on patients [
1,
4,
5]. According to a U.S. Centers for Disease Control and Prevention health care-associated infection (HAI) prevalence survey, nearly 600,000 cases of SSI occurred in the USA in 2011, making it the most common HAI [
6]. It is estimated that approximately 5% of patients develop SSI during the perioperative period, which prolongs the average length of stay by more than 9 days and increases the risk of death by 11 times [
1].
Notably, orthopedic patients have heightened susceptibility to SSI relative to other patients owing to the enduring presence of internal fixation and implant apparatus within the body [
7,
8]. These components create conducive niches and substrates for pathogenic proliferation, consequently significantly elevating the risk of postoperative wound infections [
9,
10]. When SSI occurs during joint implant surgery, the cost per treatment may exceed $90,000 [
2,
11,
12]. However, approximately 55% of SSIs are preventable through proper implementation of evidence-based strategies, so timely preoperative detection of high-risk SSI patients is critical [
13].
The National Nosocomial Infections Surveillance (NNIS) risk index [
14,
15] is the prevailing clinical prognostic instrument for predicting overall SSI risk. The NNIS system employs three autonomous and equitably significant variables—the American Society of Anesthesiology (ASA) classification [
16], surgical incision type, and operative duration—to predict SSI risk. However, the prognostic efficacy of the NNIS system remains uncertain with respect to the prediction of SSI risk in patients undergoing elective aseptic orthopedic procedures [
17,
18]. Consequently, the formulation of a composite predictive model based on multiple preoperative clinical parameters is imperative to aid orthopedic practitioners in identifying candidates at high risk of SSI.
A nomogram is a straightforward instrument for clinical prognostication and is used to predict clinical outcomes [
19]. Nomograms have extensive applications across domains, such as oncology [
20], cardiovascular ailments [
21], and other medical conditions [
22,
23]. Currently, the existing studies only focus on a certain special population, such as HIV patients [
24], or a certain type of orthopedic surgery, such as lumbar instrumentation surgery [
25], total knee arthroplasty [
26], and posterior cervical surgery [
27]. To address this, this investigation aimed to identify contributory risk factors associated with SSI in a subset of patients undergoing elective aseptic orthopedic surgery. The primary objective was to construct a predictive nomogram model for SSI risk to facilitate the identification of patients at high-risk of SSI.
Methods
Study design
This retrospective study included data from patients who underwent elective orthopedic procedures between January 2020 and December 2021. The inclusion criteria were as follows: (1) elective clean orthopedic surgery according the Centers for Disease Control and Prevention Guideline for the Prevention of Surgical Site Infection [
2], and (2) comprehensive perioperative clinical data. The exclusion criteria were as follows: (1) emergency surgery, (2) incisions not meeting type I criteria, (3) preoperative community-acquired infection, and (4) insufficient pertinent perioperative clinical data.
Patients diagnosed with SSI were exhaustively identified, and a subset of patients without SSI was randomly selected to constitute the control cohort. The entire cohort was subsequently divided into training and validation cohorts using randomized sampling. The study adhered to the principles of the Declaration of Helsinki. All clinical data were de-identified, and the requirement for informed consent was waived following ethical committee approval.
Definition of SSI
In alignment with the directives delineated in the Centers for Disease Control and Prevention and the American Academy of Orthopedic Surgery guidelines for the prevention of SSI, the diagnosis of SSI was confirmed through meticulous postoperative surveillance and adept interpretation of laboratory findings by seasoned surgical practitioners [
2,
28]. The detailed definition of SSI is that it occurs within 30 days after surgery or within 1 year after foreign body implantation, which occurs in the skin and subcutaneous tissue of the surgical incision, or in the deep soft tissue (deep fascia and muscles) related to the surgery, or surgery-related organ or cavity infections, include superficial incision infection, deep incision infection and organ cavity infection [
2].
Collection of clinical variables
Baseline patient data and perioperative outcomes were gathered by two investigators (L.Z and L.K). The collected information included demographic data, foundational laboratory parameters, surgery-associated parameters, and postoperative follow-up results. Demographic variables included age, gender, body mass index, concurrent medical conditions (e.g., hypertension, diabetes, and coronary artery disease), and the ASA class. All laboratory indices were assessed 1–3 days prior to the procedure, including creatinine, albumin, total bilirubin, direct bilirubin, alanine aminotransferase, aspartate aminotransferase, prothrombin time, red blood cell count, and platelet count, among others.
Surgery-related details included surgical category (spine, extremities, and joints), NNIS grade, operative duration, estimated blood loss, preoperative skin condition, preoperative antibiotic administration, utilization of drains, and indwelling devices. Subsequent outcomes during the postoperative follow-up encompassed the occurrence of SSI and the duration of hospital stay.
Statistical analysis
In this study, SPSS 26.0 (SPSS Inc, Chicago, IL, USA) and R Language software (version 3.5.3,
http://www.r-project.org/)) were used for statistical analysis of the clinical data of the enrolled patients. Qualitative variables are described as absolute frequencies and percentages and were compared using the Kruskal–Wallis rank sum test. Quantitative variables are expressed as mean ± standard deviation and compared using the Mann–Whitney U-test or Student’s t-test. Univariate logistic regression analysis was used to determine risk factors associated with SSI. Indices with
P < 0.1 in the univariate analysis were included in the multivariate analysis. Based on the independent risk factors screened using the results of the multivariate analysis, a nomogram model was constructed using R software. The area under the receiver operating characteristic (ROC) curve, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and other indicators were used to compare the predictive performances of the nomogram prediction model and the NNIS classification. The concordance index (C-index) and consistency calibration curves were used to analyze the predictive performance of the nomogram model. Decision curve analysis (DCA) was used to measure the clinical utility of the nomogram by calculating the net benefit of different threshold probabilities.
P < 0.05 was considered statistically significant.
Discussion
Despite advances in the management of perioperative nosocomial infections in recent years, SSIs remain a common cause of increased mortality, length of stay, and cost in surgical patients [
1,
2]. Our investigation devised a model aimed at predicting the incidence of SSI in individuals undergoing clean orthopedic surgery, thereby proficiently evaluating the risk of SSI among elective aseptic orthopedic patients. Using univariate and multivariate logistic regression analyses, we established that operation time, ASA class, and D-dimer level were independently correlated with a heightened risk of postoperative SSI. Subsequently, the logistic regression model was translated into a visual representation a nomogram. Our nomogram model not only exhibited robust predictive capability and impeccable calibration but also had substantial clinical utility in facilitating informed decision-making for patients within both the training and validation cohorts. Additionally, we extended our efforts to develop an easy-to-use and free-to-access online calculator based on the nomogram model (
https://jitao.shinyapps.io/dynnomapp/), an accessible tool designed to enable clinicians and researchers to readily ascertain the probability of postoperative SSI in the special patient populations.
The American College of Surgeons incision grading system stratifies incisions into four distinct grades: grade I, grade II, grade III, and grade IV. Grade I incisions, characterized as clean surgeries, exhibit a propensity for swift and comprehensive healing within a condensed timeframe. Directives formulated by the US Centers for Disease Control and Prevention state that clean surgeries, including of drainage procedures, require no supplementary antibiotic prophylaxis after closure of the surgical incision [
2]. Although, compared with other types of surgery, the risk of SSI in patients undergoing clean orthopedic surgery is relatively low, once SSI occurs, it may lead to serious clinical outcome [
29,
30].
The NNIS grading system is currently the most widely used clinical tool for predicting the occurrence of SSI and includes three independent and equally important variables: ASA class, surgical incision type, and surgical duration. Through the qualitative classification of these variables, the NNIS system divides the surgical risk into four levels, namely, NNIS level 0, NNIS level 1, NNIS level 2, and NNIS level 3 [
14,
15]. However, because all surgical incision types in clean surgery are the same, the NNIS system lacks specificity for clean surgery. Compared to the NNIS system, our nomogram model integrates qualitative and quantitative clinical variables. By assigning values to each clinical variable and intuitively obtaining the occurrence probability of SSI with a 95% CI, the nomogram is more convenient for orthopedic surgeons. More importantly, our predictive model had a higher predictive ability and is more suitable than traditional NNIS system for patients undergoing clean orthopedic surgery.
Similar to the NNIS system, our nomogram included the ASA class and operation time, as they were independent risk variables for SSI. ASA classification is a clinical tool used to assess the risk of developing SSI and severity of potential disease in patients undergoing preoperative anesthesia. Many studies have confirmed that ASA classification can be used for SSI risk stratification [
31‐
34]. A study of 310 patients who underwent general surgery and were classified as clean or clean-contaminated confirmed that the rate of SSI was significantly higher in patients with ASA class II-III than in patients with ASA class I (
P = 0.003). An ASA class > 2 is independently associated with SSI [
33]. The duration of surgery is another widely recognized clinical index closely related to the occurrence of SSI. In a study of 825 patients undergoing spinal surgery, operative time (
P = 0.0019) and ASA class III (
P = 0.0132) were independent risk factors for SSI [
32]. Higher ASA classes are associated with more comorbidities and poorer immunity, whereas longer operation time usually indicates higher surgical difficulty and longer incision exposure time, all of which increase the risk of pathogen invasion [
32,
35,
36]. Therefore, shortening the operation time, especially in patients with higher ASA classes, can effectively prevent SSI.
Our predictive model also incorporates another laboratory measure, the D-dimer level, which is not included in the NNIS system. Owing to the close relationship between the coagulation system, inflammation, and endothelial injury, an increase in D-dimer levels is also often observed in some non-thrombotic diseases, such as infection, surgery, trauma, heart failure, and malignant tumors [
37‐
39]. A multicenter study of patients undergoing revision total joint arthroplasty examined elevated serum C-reactive protein (CRP > 1 mg/dL), D-dimer (> 860 ng/mL), and erythrocyte sedimentation rate (> 30 mm/h), which were assigned 2, 2, and 1 points, respectively, and jointly constructed a new standard for the diagnosis of periprosthetic infection (PJI) with other laboratory indicators; its sensitivity and specificity were significantly higher than those of the Musculoskeletal Infection Association and International Consensus Conference Definition [
40]. Another study demonstrated that a serum D-dimer threshold of 0.75 mg/L predicted shoulder PJI with a sensitivity of 86%, specificity of 56%, and area under the curve of 0.74. When serum D-dimer and CRP above thresholds of 0.75 mg/L and 10 mg/L, respectively, were used to predict PJI, the sensitivity and specificity were 57% and 100%, respectively [
41]. Therefore, it is necessary to maintain D-dimer levels in patients at normal or even slightly decreased levels to reduce the incidence of SSI [
41‐
44].
This study had some limitations. First, owing to the retrospective nature of the study, it only included a small number of patients who did not develop SSI, and selection bias was inevitable. Second, some inflammatory indicators that may be related to SSI, such as C-reactive protein and procalcitonin, were missing from our study; the inclusion of these indicators may help improve the predictive accuracy of the model. Third, this was a single-center study. To verify the prediction model, we randomly divided the total cohort into training and internal validation cohorts; however, we still lacked an external validation cohort. In the future, another prospective multicenter study with a larger sample size is needed to further confirm the predictive performance of this model. Finally, models based on more advanced machine learning algorithms or radiomics may be more helpful in providing predictive model accuracy [
45‐
47]. Further development of SSI models based on other artificial intelligence is still needed to further improve prediction capabilities.
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