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19.12.2017 | Methods Paper

Analysis of the vasculature by immunohistochemistry in paraffin-embedded brains

verfasst von: Yann Decker, Andreas Müller, Eszter Németh, Walter J. Schulz-Schaeffer, Marc Fatar, Michael D. Menger, Yang Liu, Klaus Fassbender

Erschienen in: Brain Structure and Function | Ausgabe 2/2018

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Abstract

The brain vasculature can be investigated in different ways ranging from in vivo to biochemical analysis. Immunohistochemistry is a simple and powerful technique that can also be applied to archival tissues. However, staining of brain vessels on paraffin sections has been challenging. In this study, we developed an optimized method that can be used in paraffin-embedded mouse and human brain tissues derived from healthy controls and neurological disorders such as Alzheimer’s disease. We subsequently showed that this method is fully compatible with the detection of glial cells and key markers of Alzheimer’s disease including amyloid beta and phosphorylated Tau protein. Furthermore, we observed that the length of microvasculature in hippocampus of TgCRND8 Alzheimer’s disease mouse model is reduced, which is correlated with the decreased blood flow in hippocampus as determined by arterial spin labeling perfusion magnetic resonance imaging. Finally, we determined that the microvasculature length in two other Alzheimer’s disease mouse models, APP and PS1 double-transgenic mice and P301S Tau-transgenic mice, is also shortened in the dentate gyrus. Thus, we have established a new, simple and robust method to characterize the brain vasculature in the mouse and human brain.

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Titel: Analysis of the vasculature by immunohistochemistry in paraffin-embedded brains
verfasst von: Yann Decker
Andreas Müller
Eszter Németh
Walter J. Schulz-Schaeffer
Marc Fatar
Michael D. Menger
Yang Liu
Klaus Fassbender
Publikationsdatum: 19.12.2017
Verlag: Springer Berlin Heidelberg
Erschienen in: Brain Structure and Function / Ausgabe 2/2018
Print ISSN: 1863-2653
Elektronische ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-017-1595-8

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Analysis of the vasculature by immunohistochemistry in paraffin-embedded brains

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