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Erschienen in:

01.09.2009 | Original Article

Apoptosis in porcine macrophages infected in vitro with African swine fever virus (ASFV) strains with different virulence

Erschienen in: Archives of Virology | Ausgabe 9/2009

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Abstract

African swine fever virus (ASFV) replicates in porcine macrophages. Since modulation of cell death by ASFV strains of different virulence is poorly understood, we studied the development of apoptosis in porcine macrophage cultures during in vitro infection with the high- and low-virulence isolates ASFV/L60 (L60) and ASFV/NH/P68 (NHV), respectively. In cultures inoculated with each isolate, similar numbers of cells hosting infection showed morphological signs of apoptosis, which were visible from a relatively early time of infection (8 h), although a significant proportion of the infected cell populations remained non-apoptotic until 18 h. L60 inhibited caspase-3 activation by 18 h after infection and induced less DNA internucleosomic fragmentation at 8 h than NHV. However, at the late infection time, apoptosis levels were similar in both infections and occurred, at least partially, independently of caspases, suggesting the existence of yet unknown alternative pathways committing ASFV host cells to apoptotic death.

Adrain C, Murphy BM, Martin SJ (2005) Molecular ordering of the caspase activation cascade initiated by the cytotoxic T lymphocyte/natural killer (CTL/NK) protease granzyme B. J Biol Chem 280:4663–4673PubMedCrossRef

Afonso CL, Neilan JG, Kutish GF, Rock DL (1996) An African swine fever virus Bcl-2 homolog, 5-HL, suppresses apoptotic cell death. J Virol 70:4858–4863PubMed

Aubert M, Jerome KR (2003) Apoptosis prevention as a mechanism of immune evasion. Int Rev Immunol 22:361–371PubMedCrossRef

Blank M, Shiloh Y (2007) Programs for cell death: apoptosis is only one way to go. Cell Cycle 6:686–695PubMed

Borca MV, Carrillo C, Zsak L, Laegreid WW, Kutish GF, Neilan JG, Burrage TG, Rock DL (1998) Deletion of a CD2-like gene, 8-DR, from African swine fever virus affects viral infection in domestic swine. J Virol 72:2881–2889PubMed

Brookes SM, Dixon LK, Parkhouse RM (1996) Assembly of African Swine fever virus: quantitative ultrastructural analysis in vitro and in vivo. Virology 224:84–92PubMedCrossRef

Carrasco L, de Lara FC, Martin de las Mulas J, Gomez-Villamandos JC, Perez J, Wilkinson PJ, Sierra MA (1996) Apoptosis in lymph nodes in acute African swine fever. J Comp Pathol 115:415–428PubMedCrossRef

Carrascosa AL, Bustos MJ, Nogal ML, Gonzalez de Buitrago G, Revilla Y (2002) Apoptosis induced in an early step of African swine fever virus entry into Vero cells does not require virus replication. Virology 294:372–382PubMedCrossRef

Chacon MR, Almazan F, Nogal ML, Vinuela E, Rodriguez JF (1995) The African swine fever virus IAP homolog is a late structural polypeptide. Virology 214:670–674PubMedCrossRef

10.

Dixon LK, Escribano JM, Martins C, Rock DL, Salas ML, Wilkinson PJ (2005) Asfarviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy: eighth report of the international committee on taxonomy of viruses. Elsevier/Academic Press, London, pp 135–143

11.

Duarte M (2000) Bases moleculares da virulência e hemadsorção nos isolados nacionais Lisboa 60 e Lisboa 68 do vírus da peste suína Africana. PhD Thesis, Instituto de Tecnologia Química e Biológica. Universidade Nova de Lisboa, Oeiras, Portugal

12.

Ekert PG, Silke J, Vaux DL (1999) Caspase inhibitors. Cell Death Differ 6:1081–1086PubMedCrossRef

13.

Galindo I, Almazan F, Bustos MJ, Vinuela E, Carrascosa AL (2000) African swine fever virus EP153R open reading frame encodes a glycoprotein involved in the hemadsorption of infected cells. Virology 266:340–351PubMedCrossRef

14.

Galindo I, Hernaez B, Diaz-Gil G, Escribano JM, Alonso C (2008) A179L, a viral Bcl-2 homologue, targets the core Bcl-2 apoptotic machinery and its upstream BH3 activators with selective binding restrictions for Bid and Noxa. Virology 375:561–572PubMedCrossRef

15.

Gilmore AP (2005) Anoikis. Cell Death Differ 12(Suppl 2):1473–1477PubMedCrossRef

16.

Gupta S (2003) Molecular signaling in death receptor and mitochondrial pathways of apoptosis (Review). Int J Oncol 22:15–20PubMed

17.

Hernaez B, Diaz-Gil G, Garcia-Gallo M, Ignacio Quetglas J, Rodriguez-Crespo I, Dixon L, Escribano JM, Alonso C (2004) The African swine fever virus dynein-binding protein p54 induces infected cell apoptosis. FEBS Lett 569:224–228PubMedCrossRef

18.

Hernaez B, Escribano JM, Alonso C (2006) Visualization of the African swine fever virus infection in living cells by incorporation into the virus particle of green fluorescent protein-p54 membrane protein chimera. Virology 350:1–14PubMedCrossRef

19.

Hurtado C, Granja AG, Bustos MJ, Nogal ML, Gonzalez de Buitrago G, de Yebenes VG, Salas ML, Revilla Y, Carrascosa AL (2004) The C-type lectin homologue gene (EP153R) of African swine fever virus inhibits apoptosis both in virus infection and in heterologous expression. Virology 326:160–170PubMedCrossRef

20.

King KL, Jewell CM, Bortner CD, Cidlowski JA (2000) 28S ribosome degradation in lymphoid cell apoptosis: evidence for caspase and Bcl-2-dependent and -independent pathways. Cell Death Differ 7:994–1001PubMedCrossRef

21.

Leitao A, Cartaxeiro C, Coelho R, Cruz B, Parkhouse RM, Portugal F, Vigario JD, Martins CL (2001) The non-haemadsorbing African swine fever virus isolate ASFV/NH/P68 provides a model for defining the protective anti-virus immune response. J Gen Virol 82:513–523PubMed

22.

Martins CL, Scholl T, Mebus CA, Fisch H, Lawman MJ (1987) Modulation of porcine peripheral blood-derived macrophage functions by in vitro infection with African swine fever virus (ASFV) isolates of different virulence. Viral Immunol 1:177–190PubMedCrossRef

23.

McCullough KC, Basta S, Knotig S, Gerber H, Schaffner R, Kim YB, Saalmuller A, Summerfield A (1999) Intermediate stages in monocyte–macrophage differentiation modulate phenotype and susceptibility to virus infection. Immunology 98:203–212PubMedCrossRef

24.

Neilan JG, Lu Z, Afonso CL, Kutish GF, Sussman MD, Rock DL (1993) An African swine fever virus gene with similarity to the proto-oncogene bcl-2 and the Epstein-Barr virus gene BHRF1. J Virol 67:4391–4394PubMed

25.

Neilan JG, Lu Z, Kutish GF, Zsak L, Burrage TG, Borca MV, Carrillo C, Rock DL (1997) A BIR motif containing gene of African swine fever virus, 4CL, is nonessential for growth in vitro and viral virulence. Virology 230:252–264PubMedCrossRef

26.

Neilan JG, Borca MV, Lu Z, Kutish GF, Kleiboeker SB, Carrillo C, Zsak L, Rock DL (1999) An African swine fever virus ORF with similarity to C-type lectins is non-essential for growth in swine macrophages in vitro and for virus virulence in domestic swine. J Gen Virol 80(Pt 10):2693–2697PubMed

27.

Nogal ML, Gonzalez de Buitrago G, Rodriguez C, Cubelos B, Carrascosa AL, Salas ML, Revilla Y (2001) African swine fever virus IAP homologue inhibits caspase activation and promotes cell survival in mammalian cells. J Virol 75:2535–2543PubMedCrossRef

28.

O’Connell AR, Holohan C, Torriglia A, Lee BW, Stenson-Cox C (2006) Characterization of a serine protease-mediated cell death program activated in human leukemia cells. Exp Cell Res 312:27–39PubMedCrossRef

29.

Oura CA, Powell PP, Parkhouse RM (1998) African swine fever: a disease characterized by apoptosis. J Gen Virol 79(Pt 6):1427–1438PubMed

30.

Pan IC, Shimizu M, Hess WR (1980) Replication of African swine fever virus in cell cultures. Am J Vet Res 41:1357–1367PubMed

31.

Portugal R, Leitao A, Martins C (2009) Characterization of African swine fever virus IAP homologue expression in porcine macrophages infected with different virulence isolates. Vet Microbiol. doi:10.1016/j.vetmic.2009.04.030

32.

Ramiro-Ibanez F, Ortega A, Brun A, Escribano JM, Alonso C (1996) Apoptosis: a mechanism of cell killing and lymphoid organ impairment during acute African swine fever virus infection. J Gen Virol 77(Pt 9):2209–2219PubMedCrossRef

33.

Rodriguez JM, Yanez RJ, Almazan F, Vinuela E, Rodriguez JF (1993) African swine fever virus encodes a CD2 homolog responsible for the adhesion of erythrocytes to infected cells. J Virol 67:5312–5320PubMed

34.

Salguero FJ, Sanchez-Cordon PJ, Nunez A, Fernandez de Marco M, Gomez-Villamandos JC (2005) Proinflammatory cytokines induce lymphocyte apoptosis in acute African swine fever infection. J Comp Pathol 132:289–302PubMedCrossRef

35.

Trapani JA, Smyth MJ (2002) Functional significance of the perforin/granzyme cell death pathway. Nat Rev Immunol 2:735–747PubMedCrossRef

36.

Vandenabeele P, Vanden Berghe T, Festjens N (2006) Caspase inhibitors promote alternative cell death pathways. Sci STKE, 24 Oct 2006(358), pe44. doi:10.1126/stke.3582006pe44

37.

Yanez RJ, Rodriguez JM, Nogal ML, Yuste L, Enriquez C, Rodriguez JF, Vinuela E (1995) Analysis of the complete nucleotide sequence of African swine fever virus. Virology 208:249–278PubMedCrossRef

Titel: Apoptosis in porcine macrophages infected in vitro with African swine fever virus (ASFV) strains with different virulence
Publikationsdatum: 01.09.2009
Erschienen in: Archives of Virology / Ausgabe 9/2009
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-009-0466-x

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