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Archives of Virology

Erschienen in:

01.06.2011 | Original Article

Atypical characteristics of nucleoprotein of pandemic influenza virus H1N1 and their roles in reassortment restriction

verfasst von: Asawin Wanitchang, Prasatha Patarasirin, Juggragarn Jengarn, Anan Jongkaewwattana

Erschienen in: Archives of Virology | Ausgabe 6/2011

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Abstract

Sequence analysis of the nucleoprotein (NP) of swine-origin influenza virus H1N1 (S-OIV) reveals a number of atypical characteristics including an early start codon and a highly conserved, non-aromatic residue at position 313. Using an in vitro viral polymerase reconstitution assay, we found that the polymerase complex containing the NP of S-OIV (NP_S-OIV) yielded substantially lower activity than those assayed with NP derived from other influenza virus strains. Moreover, alteration of the early start codon or introduction of an aromatic residue at position 313 (V313Y) did not increase but instead exacerbated the poor polymerase activity. Interestingly, when NP_S-OIV was allowed to compete with that of a mouse-adapted influenza virus (A/PR/8/34) to form progeny virions, only progeny bearing NP_S-OIV were produced, despite the low polymerase activity associated with NP_S-OIV. Our results indicated that NP_S-OIV requires both the early start codon and the V313 residue for its optimal function. These characteristics are required for a strong compatibility between the S-OIV polymerase subunits and its indigenous NP over that of other strains, which might explain why productive reassortment between S-OIV and seasonal influenza viruses has yet to occur in nature.

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Titel: Atypical characteristics of nucleoprotein of pandemic influenza virus H1N1 and their roles in reassortment restriction
verfasst von: Asawin Wanitchang
Prasatha Patarasirin
Juggragarn Jengarn
Anan Jongkaewwattana
Publikationsdatum: 01.06.2011
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 6/2011
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-011-0947-6

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