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01.06.2011 | Original Article

The small nuclear ribonucleoprotein U1A interacts with NS5 from yellow fever virus

verfasst von: Roberta V. M. Bronzoni, Maria C. F. S. Madrid, Danilo V. B. Duarte, Vanessa O. A. Pellegrini, Carolina C. Pacca, Ana C. V. Carmo, Cleslei F. Zanelli, Sandro R. Valentini, Carolina Santacruz-Pérez, João A. R. G. Barbosa, Carol S. Lutz, Paula Rahal, Maurício L. Nogueira

Erschienen in: Archives of Virology | Ausgabe 6/2011

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Abstract

The flavivirus NS5 protein is one of the most important proteins of the replication complex, and cellular proteins can interact with it. This study shows for the first time that the yellow fever virus (YFV) NS5 protein is able to interact with U1A, a protein involved in splicing and polyadenylation. We confirmed this interaction by GST-pulldown assay and by co-immunoprecipitation in YFV-infected cells. A region between amino acids 368 and 448 was identified as the site of interaction of the NS5 protein with U1A. This region was conserved among some flaviviruses of medical importance. The implications of this interaction for flavivirus replication are discussed.

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Titel: The small nuclear ribonucleoprotein U1A interacts with NS5 from yellow fever virus
verfasst von: Roberta V. M. Bronzoni
Maria C. F. S. Madrid
Danilo V. B. Duarte
Vanessa O. A. Pellegrini
Carolina C. Pacca
Ana C. V. Carmo
Cleslei F. Zanelli
Sandro R. Valentini
Carolina Santacruz-Pérez
João A. R. G. Barbosa
Carol S. Lutz
Paula Rahal
Maurício L. Nogueira
Publikationsdatum: 01.06.2011
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 6/2011
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-011-0927-x

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