Erschienen in:
01.07.2015 | Original Article
Autophagy inhibitors reduce avian-reovirus-mediated apoptosis in cultured cells and in chicken embryos
verfasst von:
Shipeng Duan, Jinghua Cheng, Chenxi Li, Liping Yu, Xiaorong Zhang, Ke Jiang, Yupeng Wang, Jiansheng Xu, Yantao Wu
Erschienen in:
Archives of Virology
|
Ausgabe 7/2015
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Abstract
Avian reovirus (ARV)-induced apoptosis contributes to the pathogenesis of reovirus in infected chickens. However, methods for effectively reducing ARV-triggered apoptosis remain to be explored. Here, we show that pretreatment with chloroquine (CQ) or E64d plus pepstatin A decreases ARV-mediated apoptosis in chicken DF-1 cells. By acting as autophagy inhibitors, CQ and E64d plus pepstatin A increase microtubule-associated protein 1 light chain 3-II (LC3II) accumulation in ARV-infected cells, which results in decreased ARV protein synthesis and virus yield and thereby contributes to the reduction of apoptosis. Furthermore, ARV-mediated apoptosis in the bursa, heart and intestines of chicken embryos is attenuated by CQ and E64d plus pepstatin A treatment. Importantly, treatment with these autophagy inhibitors increases the survival of infected chicken embryos. Together, our data suggest that pharmacological inhibition of autophagy might represent a novel strategy for reducing ARV-mediated apoptosis.