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Archives of Virology

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01.07.2015 | Brief Report

Comparison of three magnetic-bead-based RNA extraction methods for detection of cucumber green mottle mosaic virus by real-time RT-PCR

verfasst von: Xiaoli Zhao, Qi Zhou, Lijie Zhang, Wenlong Yan, Ning Sun, Xinmiao Liang, Congliang Deng

Erschienen in: Archives of Virology | Ausgabe 7/2015

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Abstract

To determine the efficiency of RNA extraction methods based on magnetic beads, three different bead-based methods (one using silica-coated magnetic beads [SMNP], one using immunomagnetic beads conjugated to a specific antibody [IMB], and one using magnetic beads to nonspecifically adsorb virions [MNP]) were compared with the TRIzol method for the extraction of cucumber green mottle mosaic virus (CGMMV) RNA from cucumber leaves by real-time RT-PCR. The results indicated that the extraction efficiency of the SMNP method was 10 times higher than those of the IMB and MNP methods and 100 times higher than that of the TRIzol method. Therefore, the SMNP method could be considered for use in quarantine measures for the prevention and control of the disease caused by CGMMV.

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Titel: Comparison of three magnetic-bead-based RNA extraction methods for detection of cucumber green mottle mosaic virus by real-time RT-PCR
verfasst von: Xiaoli Zhao
Qi Zhou
Lijie Zhang
Wenlong Yan
Ning Sun
Xinmiao Liang
Congliang Deng
Publikationsdatum: 01.07.2015
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 7/2015
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-015-2444-9

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Comparison of three magnetic-bead-based RNA extraction methods for detection of cucumber green mottle mosaic virus by real-time RT-PCR

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