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Inflammation Research

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27.03.2024 | Original Research Paper

Bakuchicin alleviates ovalbumin-induced allergic asthma by regulating M2 macrophage polarization

verfasst von: Yeon-Yong Kim, Seungwon Jeong, Seung Woong Lee, Seung-Jae Lee, Mun-Chual Rho, Sang-Hyun Kim, Soyoung Lee

Erschienen in: Inflammation Research | Ausgabe 5/2024

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Abstract

Objective

Asthma is an airway inflammatory disease caused by activation of numerous immune cells including macrophages. Bakuchicin (BKC) is known to exhibit anti-inflammatory effects and type 2 T helper (Th2) regulation, but has not been investigated for airway inflammation. This study aimed to evaluate the effects of BKC on airway inflammation and demonstrate the mechanisms of macrophage polarization.

Methods

The anti-inflammatory effects were determined using lipopolysaccharide (LPS)-stimulated macrophages. The ovalbumin (OVA)-induced asthma mouse model was used to evaluate the effects of BKC on airway inflammation and Th2 responses. Moreover, the effect of BKC on macrophage polarization was confirmed in bone marrow-derived macrophages (BMDMs) differentiation.

Results

BKC suppressed nitric oxide production and expression of pro-inflammatory cytokines by inhibiting signaling pathway in LPS-stimulated macrophages. In an OVA-induced asthma model, BKC treatment alleviated histological changes and mast cell infiltration and reduced the levels of eosinophil peroxidase, β-hexosaminidase, and immunoglobulin levels. In addition, BKC alleviated Th2 responses and M2 macrophage populations in bronchoalveolar fluid. In BMDMs, BKC suppressed IL-4-induced M2 macrophage polarization and the expression of M2 markers such as arginase-1 and Fizz-1 through inhibiting sirtuin 2 levels.

Conclusion

BKC could be a drug candidate for the treatment of allergic asthma.

Nur mit Berechtigung zugänglich

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Titel: Bakuchicin alleviates ovalbumin-induced allergic asthma by regulating M2 macrophage polarization
verfasst von: Yeon-Yong Kim
Seungwon Jeong
Seung Woong Lee
Seung-Jae Lee
Mun-Chual Rho
Sang-Hyun Kim
Soyoung Lee
Publikationsdatum: 27.03.2024
Verlag: Springer International Publishing
Erschienen in: Inflammation Research / Ausgabe 5/2024
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-024-01859-8

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Bakuchicin alleviates ovalbumin-induced allergic asthma by regulating M2 macrophage polarization