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Medical Microbiology and Immunology
Erschienen in: Medical Microbiology and Immunology 5/2019

05.10.2018 | Original Investigation

Biogenic Au@ZnO core–shell nanocomposites kill Staphylococcus aureus without provoking nuclear damage and cytotoxicity in mouse fibroblasts cells under hyperglycemic condition with enhanced wound healing proficiency

verfasst von: Md. Imran Khan, Susanta Kumar Behera, Prajita Paul, Bhaskar Das, Mrutyunjay Suar, R. Jayabalan, Derek Fawcett, Gerrard Eddy Jai Poinern, Suraj K. Tripathy, Amrita Mishra

Erschienen in: Medical Microbiology and Immunology | Ausgabe 5/2019

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Abstract

The aim of the present study is focused on the synthesis of Au@ZnO core–shell nanocomposites, where zinc oxide is overlaid on biogenic gold nanoparticles obtained from Hibiscus Sabdariffa plant extract. Optical property of nanocomposites is investigated using UV–visible spectroscopy and crystal structure has been determined using X-ray crystallography (XRD) technique. The presence of functional groups on the surface of Au@ZnO core–shell nanocomposites has been observed by Fourier transforms infrared (FTIR) spectroscopy. Electron microscopy studies revealed the morphology of the above core–shell nanocomposites. The synthesized nanocomposite material has shown antimicrobial and anti-biofilm activity against Staphylococcus aureus and Methicillin Resistant Staphylococcus haemolyticus (MRSH). The microbes are notorious cross contaminant and are known to cause infection in open wounds. The possible antimicrobial mechanism of as synthesized nanomaterials has been investigated against Staphylococcus aureus and obtained data suggests that the antimicrobial activity could be due to release of reactive oxygen species (ROS). Present study has revealed that surface varnishing of biosynthesized gold nanoparticles through zinc oxide has improved its antibacterial proficiency against Staphylococcus aureus, whereas reducing its toxic effect towards mouse fibroblast cells under normal and hyperglycaemic condition. Further studies have been performed in mice model to understand the wound healing efficiency of Au@ZnO nanocomposites. The results obtained suggest the possible and effective use of as synthesized core shell nanocomposites in wound healing.
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Metadaten
Titel
Biogenic Au@ZnO core–shell nanocomposites kill Staphylococcus aureus without provoking nuclear damage and cytotoxicity in mouse fibroblasts cells under hyperglycemic condition with enhanced wound healing proficiency
verfasst von
Md. Imran Khan
Susanta Kumar Behera
Prajita Paul
Bhaskar Das
Mrutyunjay Suar
R. Jayabalan
Derek Fawcett
Gerrard Eddy Jai Poinern
Suraj K. Tripathy
Amrita Mishra
Publikationsdatum
05.10.2018
Verlag
Springer Berlin Heidelberg
Erschienen in
Medical Microbiology and Immunology / Ausgabe 5/2019
Print ISSN: 0300-8584
Elektronische ISSN: 1432-1831
DOI
https://doi.org/10.1007/s00430-018-0564-z

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