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Pediatric Surgery International

Erschienen in:

10.02.2021 | Original Article

Cell death induction (extrinsic versus intrinsic apoptotic pathway) by intestinal ischemia–reperfusion injury in rats is time-depended

verfasst von: Yoav Ben-Shahar, Zaid Abassi, Yulia Pollak, Tal Koppelmann, Gregory Gorelik, Igor Sukhotnik

Erschienen in: Pediatric Surgery International | Ausgabe 3/2021

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Abstract

Purpose

We investigate the mechanism of intestinal cell apoptosis and its relation to the time of reperfusion in a rat model of intestinal ischemia–reperfusion (IR).

Methods

Rats were divided into 4 groups: Sham-24 and Sham-48 rats underwent laparotomy without an intentional ischemic intervention and were sacrificed 24 or 48 h hours later; IR-24 and IR-48 rats underwent occlusion of SMA and portal vein for 20 min followed by 24 or 48 h of reperfusion, respectively. Park’s injury score, cell proliferation and apoptosis were determined at sacrifice. Proliferation and apoptosis-related gene and protein expression were determined using Real-Time PCR, Western Blot and Immunohistochemistry.

Results

IR-24 rats demonstrated a strong increase in cell apoptosis along with an elevated Bax and decreased Bcl-2 expression and a decrease in cell proliferation (vs Sham-24). IR-48 group showed an increase in cell proliferation and a decrease in cell apoptosis compared to IR-24 animals. IR-48 rats demonstrated an increase in apoptotic rate that was accompanied by greater TNF-α mRNA, Fas mRNA and FasL mRNA compared to Sham-48 animals.

Conclusion

While cell apoptosis in IR-24 rats is regulated mainly by intrinsic apoptotic pathway, 48 h followed ischemia extrinsic apoptotic pathway is responsible for pro-apoptotic effects of IR injury.

Patel A, Kaleya RN, Sammartano RJ (1992) Pathophysiology of mesenteric ischemia. Surg Clin North Am 72:31–41. https://doi.org/10.1016/s0039-6109(16)45626-4CrossRefPubMed

Metinko AP, Kunkel SL, Standiford TJ, Strieter RM (1992) Anoxia-hyperoxia induces monocyte-derived interleukin-8. J Clin Invest 90:791–798. https://doi.org/10.1172/JCI115953CrossRefPubMedPubMedCentral

Sukhotnik I, Brod V, Lurie M, Rahat MA, Shnizer S, Lahat N, Mogilner JG, Bitterman H (2009) The effect of 100% oxygen on intestinal preservation and recovery following ischemia-reperfusion injury in rats. Crit Care Med 37:1054–1061. https://doi.org/10.1097/CCM.0b013e31819d0f5cCrossRefPubMed

Menger MD, Pelikan S, Steiner D, Messmer K (1992) Microvascular ischemia-reperfusion injury in striated muscle: significance of reflow paradox. Am J Physiol Heart Circ Physiol 263:H1901–H1906. https://doi.org/10.1152/ajpheart.1992.263.6.H1901CrossRef

Noda T, Ivakiri R, Fujimoto K, Matsuo S, Aw TY (1998) Programmed cell death induced by ischemia-reperfusion in rat intestinal mucosa. Am J Physiol 274:270–276. https://doi.org/10.1152/ajpgi.1998.274.2.G270CrossRef

Buttke JM, Sandrtrom PA (1994) Oxidative stress as a mediator of apoptosis. Immunol Today 15:7–10CrossRef

Wu B, Ootani A, Iwakiri R, Fujise T, Tsunada S, Toda S, Fujimoto K (2004) Ischemic preconditioning attenuates ischemia-reperfusion-induced mucosal apoptosis by inhibiting the mitochondria-dependent pathway in rat small intestine. Am J Physiol Gastrointest Liver Physiol 286:G580–G587

Park PO, Haglund U, Bulkley GB, Fält K (1990) The sequence of development of intestinal tissue injury after strangulation ischemia and reperfusion. Surgery 107:574–580PubMed

Haglund U, Bergqvist D (1999) Intestinal ischemia-the basics. Langenbecks Arch Surg 384:233–238. https://doi.org/10.1007/s004230050197CrossRefPubMed

10.

Zimmerman BJ, Granger DN (1992) Reperfusion injury. Surg Clin North Am 72:65–83. https://doi.org/10.1016/s0039-6109(16)45628-8CrossRefPubMed

11.

Bulkley GB (1994) Reactive oxygen metabolites and reperfusion injury: aberrant triggering of reticuloendothelial function. Lancet 344:934–936. https://doi.org/10.1016/s0140-6736(94)92276-4CrossRefPubMed

12.

Langer JC, Sohal SS, Blennerhassett P (1995) Mucosal permeability after subclinical intestinal ischaemia reperfusion injury: an exploration of possible mechanisms. J Pediatr Surg 30:568–572. https://doi.org/10.1016/0022-3468(95)90133-7CrossRefPubMed

13.

Wilcox MG, Howard TJ, Plaskon LA, Unthank JL, Madura JA (1995) Current theories of pathogenesis and treatment of nonocclusive mesenteric ischemia. Dig Dis Sci 40:709–716. https://doi.org/10.1007/BF02064966CrossRefPubMed

14.

Noda T, Iwakiri R, Fujimoto K, Matsuo S, Aw TY (1998) Programmed cell death induced by ischemia-reperfusion in rat intestinal mucosa. Am J Physiol 274:G270–G276. https://doi.org/10.1152/ajpgi.1998.274.2.G270CrossRefPubMed

15.

Kerr JF, Wyllie AH, Currie AR (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26:239–257. https://doi.org/10.1038/bjc.1972.33CrossRefPubMedPubMedCentral

16.

Cavalcante GC, Schaan AP, Cabral GF, Santana-da-Silva MN, Pinto P, Vidal AF, Ribeiro-Dos-Santos Â (2019) A cell’s fate: an overview of the molecular biology and genetics of apoptosis. Int J Mol Sci 20(17):4133. https://doi.org/10.3390/ijms20174133CrossRefPubMedCentral

17.

Ashkenazi A, Dixit VM (1998) Death receptors: signaling and modulation. Science 281:1305–1308. https://doi.org/10.1126/science.281.5381.1305CrossRefPubMed

18.

Boise LH, Gottschalk AR, Quintans J, Thompson CB (1995) Bcl-2 and Bcl-2-related proteins in apoptosis regulation. Curr Top Microbiol Immunol 200:107–121. https://doi.org/10.1007/978-3-642-79437-7_8CrossRefPubMed

19.

Sukhotnik I, Slijper N, Chemodanov E, Pollak Y, Shaoul R, Coran AG, Mogilner JG (2011) Parenteral omega-3 fatty acids (Omegaven) modulate intestinal recovery following intestinal ischemia-reperfusion in a rat model. J Pediatr Surg 46:1353–1360. https://doi.org/10.1016/j.jpedsurg.2010.09.051CrossRefPubMed

20.

Slijper N, Sukhotnik I, Chemodanov E, Bashenko Y, Shaoul R, Coran AG, Mogilner J (2010) Effect of simvastatin on intestinal recovery following gut ischemia-reperfusion injury in a rat. Ped Surg International 26:105–110. https://doi.org/10.1007/s00383-009-2508-6CrossRef

21.

Sukhotnik I, Khateeb K, Mogilner JG, Helou H, Lurie M, Coran AG, Shiloni E (2007) Dietary glutamine supplementation prevents mucosal injury and modulates intestinal epithelial restitution following ischemia-reperfusion injury in the rat. Dig Dis Sci 52:1497–1504. https://doi.org/10.1007/s10620-006-9629-8CrossRefPubMed

22.

Buttke TM, Sandrtrom PA (1994) Oxidative stress as a mediator of apoptosis. Immunol Today 15:7–10. https://doi.org/10.1016/0167-5699(94)90018-3CrossRefPubMed

Titel: Cell death induction (extrinsic versus intrinsic apoptotic pathway) by intestinal ischemia–reperfusion injury in rats is time-depended
verfasst von: Yoav Ben-Shahar
Zaid Abassi
Yulia Pollak
Tal Koppelmann
Gregory Gorelik
Igor Sukhotnik
Publikationsdatum: 10.02.2021
Verlag: Springer Berlin Heidelberg
Erschienen in: Pediatric Surgery International / Ausgabe 3/2021
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-020-04817-7

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Cell death induction (extrinsic versus intrinsic apoptotic pathway) by intestinal ischemia–reperfusion injury in rats is time-depended

Abstract

Purpose

Methods

Results

Conclusion

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Purpose

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