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12.02.2021 | Original Article

Doppler ultrasound assessment of splanchnic perfusion and heart rate for the detection of necrotizing enterocolitis

verfasst von: Niloofar Ganji, Yuhki Koike, Bo Li, Haitao Zhu, Ethan Lau, Maarten Janssen Lok, Carol Lee, Agostino Pierro

Erschienen in: Pediatric Surgery International | Ausgabe 3/2021

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Abstract

Purpose

Monitoring disease progression is crucial to improve the outcome of necrotizing enterocolitis (NEC). A previous study indicates that intestinal wall flow velocity was reduced in NEC pups from the initial stages of the disease. This study aims to investigate whether splanchnic perfusion via the superior mesenteric artery (SMA) (i) is altered during NEC development and (ii) can be used as a monitoring tool to assess disease progression.

Methods

NEC was induced in C57BL/6 mice via gavage feeding of formula, hypoxia, and oral lipopolysaccharide, from postnatal day 5 (P5) to P9 (AUP: 32,238). Breastfed littermates served as controls. Doppler ultrasound (U/S) of bowel loops was performed daily. Intestinal wall perfusion was calculated as average flow velocity (mm/s) of multiple abdominal regions. Groups were compared using one-way ANOVA.

Results

The SMA flow velocity was not altered during the initial stage of NEC development, but become significantly reduced at P8 when the intestinal disease was more advanced. These changes occurred concomitantly with an increase in heart rate.

Conclusions

NEC is associated with intestinal hypo-perfusion at the periphery and flow in the SMA is reduced during the later stages of disease indicating the presence of intestinal epithelium damage. This study contributes to understanding NEC pathophysiology and illustrates the value of Doppler U/S in monitoring disease progression.

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Titel: Doppler ultrasound assessment of splanchnic perfusion and heart rate for the detection of necrotizing enterocolitis
verfasst von: Niloofar Ganji
Yuhki Koike
Bo Li
Haitao Zhu
Ethan Lau
Maarten Janssen Lok
Carol Lee
Agostino Pierro
Publikationsdatum: 12.02.2021
Verlag: Springer Berlin Heidelberg
Erschienen in: Pediatric Surgery International / Ausgabe 3/2021
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-020-04819-5

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Doppler ultrasound assessment of splanchnic perfusion and heart rate for the detection of necrotizing enterocolitis