Erschienen in:
12.06.2019 | Original Article
Development of a multiplex, PCR-based genotyping assay for African and Asian elephants for forensic purposes
verfasst von:
Margret J. Potoczniak, Michael Chermak, Lawrence Quarino, Shanan S. Tobe, Jillian Conte
Erschienen in:
International Journal of Legal Medicine
|
Ausgabe 1/2020
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Abstract
Wildlife crimes and the threats they present to elephant populations raise the need to develop and implement DNA-based methodology as an aid for wildlife forensic investigations and conservation efforts. This study describes the development of a tetra-nucleotide repeat STR multiplex, genotyping assay that will identify Asian elephant (Elephas maximus) and African elephant (Loxodonta africana) DNA. The assay targets six tetra-nucleotide STRs and two sex-typing markers simultaneously in both genera of elephants, a first for elephant genotyping assays. The developed assay has potential application in wildlife investigations to associate a biological sample to a particular individual elephant and additionally in conservation science for population management.