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Journal of Assisted Reproduction and Genetics

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01.05.2011 | Gamete biology

Developmental competence of antral follicles and their oocytes after gonadotrophin treatment of sows with gene polymorphisms for leptin and melanocortin receptors (Iberian pig)

verfasst von: Jorge Muñoz-Frutos, Teresa Encinas, Pilar Pallares, Laura Torres-Rovira, Pedro Gonzalez-Añover, Emilio Gomez-Izquierdo, Raul Sanchez-Sanchez, Antonio Gonzalez-Bulnes

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 5/2011

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Abstract

Purpose

To evaluate possible differences in follicle and oocyte developmental competence after gonadotrophin treatment in sows of obese and lean genotypes.

Methods

Follicle dynamics, ovulation rate and oocyte developmental competence to embryo were compared between females, of obese (n = 7) and lean genotypes (n = 10), treated with 1,250 I.U. of eCG and 500 I.U. of hCG.

Results

The obese genotype showed lower numbers of follicles growing to preovulatory stages (12.4 ± 1.8 vs 18.6 ± 1.0, P < 0.05), of corpora lutea (16.0 ± 0.9 vs 23.5 ± 0.9, P < 0.05), and of recovered oocytes/embryos (8.0 ± 1.3 vs 12.9 ± 0.9, P < 0.05). Thereafter, embryo viability rates also decreased when compared to lean genotypes (62.5 vs 77.6%, P < 0.05).

Discussion

To our knowledge, this is the first study analyzing the effect of obese genotypes on the ovarian response to exogenous gonadotrophins in a non-rodent animal model, the pig. A lower efficiency of gonadotrophin treatments for stimulation of follicle development and induction of ovulation was observed.

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Titel: Developmental competence of antral follicles and their oocytes after gonadotrophin treatment of sows with gene polymorphisms for leptin and melanocortin receptors (Iberian pig)
verfasst von: Jorge Muñoz-Frutos
Teresa Encinas
Pilar Pallares
Laura Torres-Rovira
Pedro Gonzalez-Añover
Emilio Gomez-Izquierdo
Raul Sanchez-Sanchez
Antonio Gonzalez-Bulnes
Publikationsdatum: 01.05.2011
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 5/2011
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9535-7

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Developmental competence of antral follicles and their oocytes after gonadotrophin treatment of sows with gene polymorphisms for leptin and melanocortin receptors (Iberian pig)