nach oben

International Journal of Legal Medicine

Erschienen in:

10.12.2019 | Original Article

DNA barcoding of Stearibia nigriceps (Meigen) and Piophila casei (Linnaeus) (Diptera: Piophilidae) from Algeria and the first African report of Stearibia nigriceps

verfasst von: Meriem Taleb, Ghania Tail, Halide Nihal Açıkgöz

Erschienen in: International Journal of Legal Medicine | Ausgabe 3/2020

Einloggen, um Zugang zu erhalten

Abstract

Stearibia (=Piophila) nigriceps (Meigen) occurs in the Palearctic Region from Europe and Far East Russia, and in the Nearctic, the Neotropical, and the Oriental regions. In this study, we report the first record of Stearibia nigriceps from Africa collected in Algeria. In addition, our results provide DNA sequences of Piophila casei (Linnaeus) and S. nigriceps that may serve as reference data for future identification. A region of the cytochrome c oxidase I (COI) gene marker of 473 bp was amplified and DNA was sequenced. Intra- and interspecific genetic distances were calculated. A phylogenetic tree was generated by the maximum likelihood (ML) method using 1000 bootstrap replicates based on the Tamura-Nei model. A total number of 9 adults of S. nigriceps were collected together with 72 adults of P. casei. The intraspecific variation of the species barcodes analysed in our study was < 3% and the interspecific nucleotide divergence of the same species was > 3%. The 473 bp COI region was sufficient to identify and distinguish unambiguously between the two species. P. casei and P. nigriceps individuals from Algeria clustered separately from each other. They were also separated from the individuals of the same species originating from other countries with a 100% high bootstrap value. There was no correlation between the clades and the geographic origins of the haplotypes. Our results contribute to update the knowledge of the distribution of Piophilidae family. They also contribute to the build-up of online DNA sequence databases.

Martín-Vega D (2011) Skipping clues: forensic importance of the family Piophilidae (Diptera). Forensic Sci Int 212:1–5. https://doi.org/10.1016/j.forsciint.2011.06.016 CrossRefPubMed

Byrd JH, Castner JL (2001) Forensic entomology: the utility of arthropods in legal investigations. CRC Press, Boca Raton

Gennard D (2012) Forensic entomology: an introduction, 2nd edn. Wiley-Blackwell, Chichester

Anderson GS, VanLaerhoven SL (1996) Initial studies on insect succession on carrion in southwestern British Columbia. J Forensic Sci 41:617–625. https://doi.org/10.1520/JFS13964J CrossRef

Bonduriansky R, Brooks RJ (1999) Reproductive allocation and reproductive ecology of seven species of Diptera. Ecol Entomol 24:389–395. https://doi.org/10.1046/j.1365-2311.1999.00221.x CrossRef

Tabor KL, Fell RD, Brewster CC (2005) Insect fauna visiting carrion in Southwest Virginia. Forensic Sci Int 150:73–80. https://doi.org/10.1016/j.forsciint.2004.06.041 CrossRefPubMed

Matuszewski S, Bajerlein D, Konwerski S, Szpila K (2008) An initial study of insect succession and carrion decomposition in various forest habitats of Central Europe. Forensic Sci Int 180:61–69. https://doi.org/10.1016/j.forsciint.2008.06.015 CrossRefPubMed

Prado e Castro C, Serrano A, Martins da Silva P, Garcia MD (2012) Carrion flies of forensic interest: a study of seasonal community composition and succession in Lisbon, Portugal. Med Vet Entomol 26:417–431. https://doi.org/10.1111/j.1365-2915.2012.01031.x CrossRefPubMed

Watson EJ, Carlton CE (2003) Spring succession of necrophilous insects on wildlife carcasses in Louisiana. J Med Entomol 40:338–347. https://doi.org/10.1603/0022-2585-40.3.338 CrossRefPubMed

10.

Grisales D, Ruiz M, Villegas S (2010) Insects associated with exposed decomposing bodies in the Colombian Andean Coffee Region. Rev Bras Entomol 54:637–644. https://doi.org/10.1590/S0085-56262010000400016 CrossRef

11.

Lefebvre F, Gaudry E (2009) Forensic entomology: a new hypothesis for the chronological succession pattern of necrophagous insect on human corpses. Ann Soc Entomol Fr 45:377–392. https://doi.org/10.1080/00379271.2009.10697622 CrossRef

12.

Wyss C, Cherix D (2013) Traité d’entomologie forensique: les insectes sur la scène de crime, 2nd edn. Presses polytechniques et universitaires romandes, Lausanne

13.

Smith KGV (1986) A manual of forensic entomology. The British Museum (Natural History), London

14.

Prado e Castro C, Cunha E, Serrano A, García MD (2012) Piophila megastigmata (Diptera: Piophilidae): first records on human corpses. Forensic Sci Int 214:23–26. https://doi.org/10.1016/j.forsciint.2011.07.009 CrossRefPubMed

15.

McAlpine JF (1977) A revised classification of the Piophilidae, including ‘Neottiophilidae’ and ‘Thyreophoridae’ (Diptera: Schizophora). Mem Entomol Soc Canada 103:1–66. https://doi.org/10.4039/entm109103fv

16.

Rochefort S, Giroux M, Savage J, Wheeler TA (2015) Key to forensically important Piophilidae (Diptera) in the Nearctic Region. Can J Arthropod Identif 27:1–37. https://doi.org/10.3752/cjai.2015.27 CrossRef

17.

Misiachna AV, Korneyev VA (2015) The first records of the skipper flies (Diptera, Piophilidae) from Ukraine. Vestn Zool 49:113–118. https://doi.org/10.1515/vzoo-2015-0012 CrossRef

18.

Merz B (1996) Die Piophilidae (Diptera) der Schweiz mit Beschreibung einer neuen Art. Mitteilungen der Schweizerischen Entomol Gesellschaft 69:345–360

19.

Hrdinová M, Šuláková H, Barták M (2013) Využití čeledi Piophilidae (Diptera) ve forenzní praxi. In: Kubík Š, Barták M (eds) Workshop on biodiversity, Jevany. Czech Academy of Agricultural Sciences, Prague, pp 170–184

20.

Turchetto M, Lafisca S, Costantini G (2001) Postmortem interval (PMI) determined by study sarcophagous biocenoses: three cases from the province of Venice (Italy). Forensic Sci Int 120:28–31. https://doi.org/10.1016/S0379-0738(01)00412-1 CrossRefPubMed

21.

Fiedler A, Halbach M, Sinclair B, Benecke M (2008) What is the edge of a forest? A diversity analysis of adult Diptera found on decomposing piglets inside and on the edge of a western German woodland inspired by a courtroom question. Entomol Heute 20:173–191

22.

Baumjohann K, Rudzinski H-G (2012) Bemerkenswerte Fliegen-Funde (Diptera) im Rahmen einer forensisch entomologischen Freilandstudie in Nordrhein-Westfalen (Deutschland). Stud Dipterol 19:9–16

23.

Martín-Vega D, Gómez-Gómez A, Baz A (2010) The Austrian Piophilidae (Diptera) in the collection of the National Museum of Natural Sciences of Madrid, Spain. Beiträge Zur Entomofaunistik 11:9–12. https://doi.org/10.1049/iet-rsn.2008.0141 CrossRef

24.

Marchenko MI (2001) Medicolegal relevance of cadaver entomofauna for the determination of the time of death. Forensic Sci Int 120:89–109. https://doi.org/10.1016/S0379-0738(01)00416-9 CrossRefPubMed

25.

Duda O (1924) Revision der europäischen u. grönländischen sowie einiger südostasiat. Arten der Gattung Piophila Fallén (Dipteren). Konowia (Vienna) 3:97–113

26.

Reed HB Jr (1958) A study of dog carcass communities in Tennessee, with special reference to the insects. Am Midl Nat 59:213–245. https://doi.org/10.2307/2422385 CrossRef

27.

Michaud J-P, Majka CG, Privé J-P, Moreau G (2010) Natural and anthropogenic changes in the insect fauna associated with carcasses in the North American Maritime lowlands. Forensic Sci Int 202:64–70. https://doi.org/10.1016/j.forsciint.2010.04.028 CrossRefPubMed

28.

Wolff M, Pérez S, Grisales D (2016) Family Piophilidae. Zootaxa 4122:576–578. https://doi.org/10.11646/zootaxa.4122.1.46 CrossRefPubMed

29.

Sathe TV, Sathe A, Sathe NT (2013) Diversity of dipterous forensic insects from Western Maharashtra, India. Int J Pharm Bio Sci 4:173–179

30.

Cainé LM, Real FC, Saloña-Bordas MI, de Pancorbo MM, Lima G, Magalhães T, Pinheiro F (2009) DNA typing of Diptera collected from human corpses in Portugal. Forensic Sci Int 184:e21–e23. https://doi.org/10.1016/j.forsciint.2008.10.016 CrossRefPubMed

31.

Wells JD, Introna F Jr, DiVella G et al (2001) Human and insect mitochondrial DNA analysis from maggots. J Forensic Sci 46:685–687PubMed

32.

Cainé L, Real FC, Lima G et al (2006) Genetic identification of forensically important Calliphoridae in Portugal. Int Congr Ser 1288:846–848. https://doi.org/10.1016/j.ics.2005.09.153 CrossRef

33.

Salem AM, Adham FK, Picard CJ (2015) Survey of the genetic diversity of forensically important Chrysomya (Diptera: Calliphoridae) from Egypt. J Med Entomol 52:320–328. https://doi.org/10.1093/jme/tjv013 CrossRefPubMed

34.

Guo YD, Cai JF, Meng FM et al (2011) Identification of forensically important flesh flies based on a shorter fragment of the cytochrome oxidase subunit I gene in China. Medic 26:307–313. https://doi.org/10.1111/j.1365-2915.2011.01003.x CrossRef

35.

Arnaldos M-I, Ruiz C, Torres B et al (2015) DNA barcoding of two forensically important fleshfly species (Diptera: Sarcophagidae) from Spain and notes on barcoding success within genus Sarcophaga Meigen, 1826. Ciencia Forense 12:73–90

36.

Grzywacz A, Wyborska D, Piwczyński M (2017) DNA barcoding allows identification of European Fanniidae (Diptera) of forensic interest. Forensic Sci Int 278:106–114. https://doi.org/10.1016/j.forsciint.2017.06.023 CrossRefPubMed

37.

Zajac BK, Martin-Vega D, Feddern N, Fremdt H, e Castro CP, Szpila K, Reckel F, Schütt S, Verhoff MA, Amendt J, Zehner R (2016) Molecular identification and phylogenetic analysis of the forensically important family Piophilidae (Diptera) from different European locations. Forensic Sci Int 259:77–84. https://doi.org/10.1016/j.forsciint.2015.12.024 CrossRefPubMed

38.

Boehme P, Amendt J, Disney RHL, Zehner R (2010) Molecular identification of carrion-breeding scuttle flies (Diptera: Phoridae) using COI barcodes. Int J Legal Med 124:577–581. https://doi.org/10.1007/s00414-010-0429-5 CrossRefPubMed

39.

Sperling FA, Anderson GS, Hickey DA (1994) A DNA-based approach to the identification of insect species used for postmortem interval estimation. J Forensic Sci 39:418–427CrossRefPubMed

40.

Wells JD, Williams DW (2007) Validation of a DNA-based method for identifying Chrysomyinae (Diptera: Calliphoridae) used in a death investigation. Int J Legal Med 121:1–8. https://doi.org/10.1007/s00414-005-0056-8 CrossRefPubMed

41.

Taleb M, Tail G, Açikgöz HN (2018) First record of Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) in Algeria. Entomol News 128:78–86CrossRef

42.

Simon C, Frati F, Beckenbach A et al (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am 87:651–701. https://doi.org/10.1093/aesa/87.6.651 CrossRef

43.

Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874. https://doi.org/10.1093/molbev/msw054 CrossRefPubMedPubMedCentral

44.

Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797. https://doi.org/10.1093/nar/gkh340 CrossRefPubMedPubMedCentral

45.

Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526. https://doi.org/10.1093/oxfordjournals.molbev.a040023 CrossRefPubMed

46.

Sikes DS, Bowser M, Daly K et al (2017) The value of museums in the production, sharing, and use of entomological data to document hyperdiversity of the changing North. Arctic Sci 3:498–514. https://doi.org/10.1139/as-2016-0038 CrossRef

47.

Han HY, Ro KE (2016) Molecular phylogeny of the superfamily Tephritoidea (Insecta: Diptera) reanalysed based on expanded taxon sampling and sequence data. J Zool Syst Evol Res 54:276–288. https://doi.org/10.1111/jzs.12139 CrossRef

48.

Ahmad A, Ahmad AH, Dieng H, Satho T, Ahmad H, Aziz AT, Boots M (2011) Cadaver wrapping and arrival performance of adult flies in an oil palm plantation in northern peninsular Malaysia. J Med Entomol 48:1236–1246. https://doi.org/10.1603/ME10247 CrossRefPubMed

49.

McAlpine JF (1978) A new species of Piophila from South Africa (Diptera: Piophilidae). Ann Natal Museum 23:455–459

50.

Saifi M, Doumandji S, Martín-Vega D (2016) Piophila megastigmata McAlpine 1978 (Diptera: Piophilidae): the first record from northern Africa of this forensically relevant fly. Ann Soc Entomol Fr 52:243–246. https://doi.org/10.1080/00379271.2016.1244491 CrossRef

51.

Martín-Vega D, Baz A, Michelsen V (2010) Back from the dead: Thyreophora cynophila (Panzer, 1798) (Diptera: Piophilidae) ‘globally extinct’ fugitive in Spain. Syst Entomol 35:607–613. https://doi.org/10.1111/j.1365-3113.2010.00541.x CrossRef

52.

Gómez-Gómez A, Díaz-Aranda LM, Michelsen V (2008) Rediscovery of Centrophlebomyia furcata (Fabricius, 1794) (Diptera: Piophilidae) in Europe. Stud Dipterol 15:237–241

53.

Baz A, Martín-Vega D (2011) Could the ‘vulture restaurants’ be a lifeboat for the recently rediscovered bone-skippers (Diptera: Piophilidae)? J Insect Conserv 15:747–753. https://doi.org/10.1007/s10841-011-9429-0 CrossRef

54.

Hebert PDN, Cywinska A, Ball SL, DeWaard JR (2003) Biological identifications through DNA barcodes. Proc R Soc Lond B 270:313–321. https://doi.org/10.1098/rspb.2002.2218 CrossRef

55.

Avise JC (2000) Phylogeography: the history and formation of species. Harvard University Press, Cambridge

56.

Tantawi TI, Whitworth TL, Sinclair BJ (2017) Revision of the Nearctic Calliphora Robineau-Desvoidy (Diptera: Calliphoridae). Zootaxa 4226:301–347. https://doi.org/10.11646/zootaxa.4226.3.1 CrossRef

57.

Harvey ML, Dadour IR, Gaudieri S (2003) Mitochondrial DNA cytochrome oxidase I gene: potential for distinction between immature stages of some forensically important fly species (Diptera) in western Australia. Forensic Sci Int 131:134–139. https://doi.org/10.1016/S0379-0738(02)00431-0 CrossRefPubMed

58.

Sharma S, Singh D (2014) Mitochondrial DNA of two forensically important species of Chrysomya (Diptera: Calliphoridae) from India. Orient Insects 48:316–321. https://doi.org/10.1080/00305316.2015.1013181 CrossRef

59.

Harvey ML, Gaudieri S, Villet MH, Dadour IR (2008) A global study of forensically significant calliphorids: implications for identification. Forensic Sci Int 177:66–76. https://doi.org/10.1016/j.forsciint.2007.10.009 CrossRefPubMed

60.

Reibe S, Schmitz J, Madea B (2009) Molecular identification of forensically important blowfly species (Diptera: Calliphoridae) from Germany. Parasitol Res 106:257–261. https://doi.org/10.1007/s00436-009-1657-9 CrossRefPubMed

61.

Stevens JR, Wall R, Wells JD (2002) Paraphyly in Hawaiian hybrid blowfly populations and the evolutionary history of anthropophilic species. Insect Mol Biol 11:141–148. https://doi.org/10.1046/j.1365-2583.2002.00318.x CrossRefPubMed

Titel: DNA barcoding of Stearibia nigriceps (Meigen) and Piophila casei (Linnaeus) (Diptera: Piophilidae) from Algeria and the first African report of Stearibia nigriceps
verfasst von: Meriem Taleb
Ghania Tail
Halide Nihal Açıkgöz
Publikationsdatum: 10.12.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: International Journal of Legal Medicine / Ausgabe 3/2020
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-019-02223-w

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

DNA barcoding of Stearibia nigriceps (Meigen) and Piophila casei (Linnaeus) (Diptera: Piophilidae) from Algeria and the first African report of Stearibia nigriceps

Abstract

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2020

A stepwise strategy to distinguish menstrual blood from peripheral blood by Fisher’s discriminant function

Mongolian spots as a finding in forensic examinations of possible child abuse–implications for case work

Communicating 3D data—interactive 3D PDF documents for expert reports and scientific publications in the field of forensic medicine

Feasibility of an accelerated PVAL method for the collection of GSR and biological traces

The effect of bleaching agents on the DNA analysis of bloodstains on different floor coverings

Developmental validation study of a 24-plex Y-STR direct amplification system for forensic application

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie