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01.12.2012 | Research | Ausgabe 1/2012 Open Access

Journal of Inflammation 1/2012

Doxycycline impairs neutrophil migration to the airspaces of the lung in mice exposed to intratracheal lipopolysaccharide

Zeitschrift:
Journal of Inflammation > Ausgabe 1/2012
Autoren:
Andres Moon, Sucheol Gil, Sean E Gill, Peter Chen, Gustavo Matute-Bello
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1476-9255-9-31) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

AM performed the animal experiments and participated in the drafting of the manuscript. SG performed immunoassays and flow cytometry and participated in the drafting of the manuscript. SEG performed the chemotaxis assays and participated in editing the manuscript. PC performed syndecan-1 dot blot assays and participated in the drafting of the manuscript. GMB conceived the study, participated in its design and coordination and drafted the manuscript. All the authors approved the final version of the manuscript.

Abstract

Background

Tetracyclines are broad-spectrum antibiotics that are also used to induce gene expression using the reverse tetracycline transactivator / tetracycline operator system (rtTA/tetO system). The system assumes that tetracyclines have no effects on mammals. However, a number of studies suggest that tetracyclines may have powerful anti-inflammatory effects. We report that the tetracycline, doxycycline, inhibits neutrophil (PMN) influx into the lungs of mice treated with bacterial endotoxin (LPS).

Methods

Mice were challenged with intratracheal LPS in the presence or absence of doxycyline. bronchoalveolar lavage cell counts and differential, total bronchoalveolar lavage protein, lung homogenate caspase-3 and tissue imaging were used to assess lung injury. In addition, PMN chemotaxis was measured in vitro and syndecan-1 was measured in bronchoalveolar lavage fluid.

Results

The administration of doxycycline resulted in a significant decrease in the number of bronchoalveolar lavage PMNs in LPS-treated mice. Doxycycline had no effect on other markers of lung injury such as total bronchoalveolar lavage protein and whole lung caspase-3 activity. However, doxycycline resulted in a decrease in shed syndecan-1 in bronchoalveolar lavage fluid.

Conclusion

We conclude that doxycycline has an important anti-inflammatory effect that can potentially confound the experiments in which the rtTA/tetO system is being used to study the immune response.
Zusatzmaterial
Authors’ original file for figure 1
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Authors’ original file for figure 6
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Literatur
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