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16.11.2016 | original article | Ausgabe 1/2017

European Surgery 1/2017

Effect of different doses of 2‑aminoethoxydiphenyl borate on intestinal ischemia-reperfusion injury

Zeitschrift:
European Surgery > Ausgabe 1/2017
Autoren:
Assoc. Prof. Murat Basbug, Assist. of Prof. Murat Yildar, Assoc. of Prof. İsmail Yaman, Assist. of Prof. Faruk Cavdar, Assoc. of Prof. Ömer Faruk Özkan, Assoc. of Prof. Hasan Aksit, Assoc. of Prof. Musa Ozgür Ozyigit, Assist. of Prof. Figen Aslan, Prof. Hayrullah Derici
Wichtige Hinweise
Author contribution M. Basbug, M. Yildar, F. Cavdar, I. Yaman, and O.F. Ozkan performed the surgical procedure; H. Derici contributed to writing the article and the review of the literature, as well as undertaking a comprehensive literature search; F. Aslan and O. Yigit provided the histopathological information and H. Aksit analyzed the biochemical parameters.

Summary

Background

Acute mesenteric ischemia is a life-threatening clinical entity. 2‑Aminoethoxydiphenyl borate (2-APB) is a membrane-permeable modulator of intracellular inositol triphosphate-induced calcium release. We investigated the effects of different 2‑APB doses on intestinal ischemia-reperfusion injury in an experimental rat model.

Methods

We divided 24 Wistar albino rats into four groups: sham, control, ischemia-reperfusion +2 mg/kg 2‑APB, and ischemia-reperfusion +4 mg/kg 2‑APB. The sham group only underwent laparotomy for 1 h 30 min. A 30-min period of mesenteric ischemia was induced in the control and two treatment groups, followed by 1 h of reperfusion. Before the laparotomy, 2 mg/kg and 4 mg/kg 2‑APB was administered i.v. in the treatments groups, and blood samples were collected after reperfusion. Serum levels of malondialdehyde, superoxide dismutase, glutathione, total antioxidant capacity, tumor necrosis factor (TNF)-α, and interleukin-6 were analyzed. Intestinal tissues were taken for histopathological, DNA fragmentation, and terminal deoxynucleotidyl transferase dUTP nick end labeling analyses to determine the proportion of apoptotic cells.

Results

2-APB reduced serum malondialdehyde, TNF-α, and interleukin-6 levels. However, superoxide dismutase and total antioxidant capacity levels increased significantly in the 4‑mg/kg 2‑APB group (p < 0.05). The intestinal histopathological injury scores were significantly higher in the control group; these injuries were prevented in the 4‑mg/kg 2‑APB dose group. DNA damage after ischemia-perfusion decreased significantly in the 4‑mg/kg 2‑APB group compared with the control group.

Conclusion

2-APB decreases oxidative stress and cell injury. Administering 4 mg/kg 2‑APB prevented ischemia-perfusion injury by diminishing histological damage.

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