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01.12.2015 | Original Article

Effects of low-level laser therapy on the expression of osteogenic genes during the initial stages of bone healing in rats: a microarray analysis

verfasst von: Carla Roberta Tim, Paulo Sérgio Bossini, Hueliton Wilian Kido, Iran Malavazi, Marcia Regina von Zeska Kress, Marcelo Falsarella Carazzolle, Nivaldo Antonio Parizotto, Ana Cláudia Rennó

Erschienen in: Lasers in Medical Science | Ausgabe 9/2015

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Abstract

This study evaluated the morphological changes produced by LLLT on the initial stages of bone healing and also studied the pathways that stimulate the expression of genes related to bone cell proliferation and differentiation. One hundred Wistar rats were divided into control and treated groups. Noncritical size bone defects were surgically created at the upper third of the tibia. Laser irradiation (Ga-Al-As laser 830 nm, 30 mW, 94 s, 2.8 J) was performed for 1, 2, 3, 5, and 7 sessions. Histopathology revealed that treated animals produced increased amount of newly formed bone at the site of the injury. Moreover, microarray analysis evidenced that LLLT produced a significant increase in the expression TGF-β, BMP, FGF, and RUNX-2 that could stimulate osteoblast proliferation and differentiation, which may be related to improving the deposition of newly formed bone at the site of the injury. Thus, it is possible to conclude that LLLT improves bone healing by producing a significant increase in the expression of osteogenic genes.

Sella VR, do Bomfim FR, Machado PC, da Silva Morsoleto MJ, Chohfi M, Plapler H (2015) Effect of low-level laser therapy on bone repair: a randomized controlled experimental study. Lasers Med Sci. [Epub ahead of print]

Claes L, Willie B (2007) The enhancement of bone regeneration by ultrasound. Prog Biophys Mol Biol 93:384–98CrossRefPubMed

Einhorn TA, Gerstenfeld LC (2015) Fracture healing: mechanisms and interventions. Nat Rev Rheumatol 11(1):45–54PubMedCentralCrossRefPubMed

Hoppe A, Güldal NS, Boccaccini AR (2011) A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials 32(11):2757–74CrossRefPubMed

Rodrigues NC, Assis L, Fernandes KR, Magri A, Ribeiro DA, Brunelli R, Abreu DC, Renno AC (2013) Effects of 660 nm low-level laser therapy on muscle healing process after cryolesion. J Rehabil Res Dev 50(7):985–96CrossRefPubMed

Carrinho PM, Renno AC, Koeke P, Salate AC, Parizotto NA, Vidal BC (2006) Comparative study using 685-nm and 830-nm lasers in the tissue repair of tenotomized tendons in the mouse. Photomed Laser Surg 24:754–8CrossRefPubMed

Oliveira P, Santos AA, Rodrigues T, Tim CR, Pinto KZ, Magri AM, Fernandes KR, Mattiello SM, Parizotto NA, Anibal FF, Rennó AC (2013) Effects of phototherapy on cartilage structure and inflammatory markers in an experimental model of osteoarthritis. J Biomed Opt 18:128004CrossRefPubMed

Bossini PS, C, Ribeiro DA, Fangel R, C, Lahoz M De A, Parizotto NA (2012) Low level laser therapy (830nm) improves bone repair in osteoporotic rats: similar outcomes at two different dosages. Exp Gerontol 2:136–142

Tim CR, Pinto KN, Rossi BR, Fernandes K, Matsumoto MA, Parizotto NA, Rennó AC (2014) Low-level laser therapy enhances the expression of osteogenic factors during bone repair in rats. Lasers Med Sci 1:147–56CrossRef

10.

Karu TI, Piatibrat LV, Kalendo GS (1999) Suppression of the intracellular concentration of ATP by irradiating with a laser pulse of wavelength lambda = 820 nm. Dokl Akad Nauk 364:399–401PubMed

11.

Buravlev EA, Zhidkova TV, Osipov AN, Vladimirov YA (2015) Are the mitochondrial respiratory complexes blocked by NO the targets for the laser and LED therapy? Lasers Med Sci 30:173–80CrossRefPubMed

12.

Stein A, Benayahu D, Maltz L, Oron U (2005) Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg 23:161–6CrossRefPubMed

13.

Fávaro-Pípi E, Ribeiro DA, Ribeiro JU, Bossini P, Oliveira P, Parizotto NA, Tim C, de Araújo HS, Renno AC (2011) Low-level laser therapy induces differential expression of osteogenic genes during bone repair in rats. Photomed Laser Surg 29:311–7CrossRefPubMed

14.

Wu YH, Wang J, Gong DX, Gu HY, Hu SS, Zhang H (2012) Effects of low-level laser irradiation on mesenchymal stem cell proliferation: a microarray analysis. Lasers Med Sci 27:509–19CrossRefPubMed

15.

Laraia EM, Silva IS, Pereira DM, Dos Reis FA, Albertini R, De Almeida P, Leal Junior EC, De Tarso Camillo De Carvalho P (2012) Effect of low-level laser therapy (660 nm) on acute inflammation induced by tenotomy of Achilles tendon in rats. Photochem Photobiol 88:1546–50CrossRefPubMed

16.

de Lima FM, Villaverde AB, Albertini R, Corrêa JC, Carvalho RL, Munin E, Araújo T, Silva JA, Aimbire F (2011) Dual Effect of low-level laser therapy (LLLT) on the acute lung inflammation induced by intestinal ischemia and reperfusion: action on anti- and pro-inflammatory cytokines. Lasers Surg Med 43:410–20CrossRefPubMed

17.

Garavello I, Baranauskas V, da Cruz-Höfling MA (2004) The effects of low laser irradiation on angiogenesis in injured rat tibiae. Histol Histopathol 19(1):43–8PubMed

18.

Assis L, Moretti AI, Abrahão TB, Cury V, Souza HP, Hamblin MR, Parizotto NA (2012) Low-level laser therapy (808 nm) reduces inflammatory response and oxidative stress in rat tibialis anterior muscle after cryolesion. Lasers Surg Med 44:726–35PubMedCentralCrossRefPubMed

19.

da Silva AP, Petri AD, Crippa GE, Stuani AS, Stuani AS, Rosa AL, Stuani MB (2012) Effect of low-level laser therapy after rapid maxillary expansion on proliferation and differentiation of osteoblastic cells. Lasers Med Sci 27:777–83CrossRefPubMed

20.

de Castro PA, Savoldi M, Bonatto D, Malavazi I, Goldman MH, Berretta AA, Goldman GH (2012) Transcriptional profiling of Saccharomyces cerevisiae exposed to propolis. BMC Complement Altern Med 24:194CrossRef

21.

Soleimani M, Abbasnia E, Fathi M, Sahraei H, Fathi Y, Kaka G (2012) The effects of low-level laser irradiation on differentiation and proliferation of human bone marrow mesenchymal stem cells into neurons and osteoblasts—an in vitro study. Lasers Med Sci 27:423–30CrossRefPubMed

22.

Medina-Huertas R, Manzano-Moreno FJ, De Luna-Bertos E, Ramos-Torrecillas J, García-Martínez O, Ruiz C. The effects of low-level diode laser irradiation on differentiation, antigenic profile, and phagocytic capacity of osteoblast-like cells (MG-63). Lasers Med Sci 29:1479–1484

23.

Matsumoto MA, Ferino FV, Monteleone GF, Ribeiro DA (2009) Low level laser therapy modulates cyclo-oxygenase-2 expression during bone repair in rats. Lasers Med Sci 24:196–201CrossRef

24.

Altan AB, Bicakci AA, Avunduk MC, Esen H (2015) The effect of dosage on the efficiency of LLLT in new bone formation at the expanded suture in rats. Lasers Med Sci 30:255–62CrossRefPubMed

25.

Kulterer B, Friedl G, Jandrositz A, Sanchez-Cabo F, Prokesch A, Paar C, Scheideler M, Windhager R, Preisegger KH, Trajanoski Z (2007) Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation. BMC Genomics 12:8–70

26.

Li WG, Xu XX (2005) The expression of N-cadherin, fibronectin during chondrogenic differentiation of MSC induced by TGF-beta(1). Chin J Traumatol 8:349–51PubMed

27.

Pyo SJ, Song WW, Kim IR, Park BS, Kim CH, Shin SH, Chung IK, Kim YD (2013) Low-level laser therapy induces the expressions of BMP-2, osteocalcin, and TGF-β1 in hypoxic-cultured human osteoblasts. Lasers Med Sci 2:543–50CrossRef

28.

Chen MH, Huang YC, Sun JS, Chao YH, Chen MH (2014) Second messengers mediating the proliferation and collagen synthesis of tenocytes induced by low-level laser irradiation. Lasers Med Sci 30:263–72CrossRefPubMed

29.

Bessa PC, Casal M, Reis RL (2008) Bone morphogenetic proteins in tissue engineering: the road from the laboratory to the clinic, part I (basic concepts). J Tissue Eng Regen Med 2:1–13CrossRefPubMed

30.

Reddi AH (2001) Bone morphogenetic proteins: from basic science to clinical applications. J Bone Joint Surg 83-A:S1–6PubMed

31.

Kloen P, Di Paola M, Borens O, Richmond J, Perino G, Helfet DL, Goumans MJ (2003) BMP signaling components are expressed in human fracture callus. Bone 33:362–71CrossRefPubMed

32.

Ducy P (2000) Cbfa1: a molecular switch in osteoblast biology. Dev Dyn 219:461–71CrossRefPubMed

33.

Canalis E, Economides AN, Gazzerro E (2003) Bone morphogenetic proteins, their antagonists, and the skeleton. Endocr Rev 24:218–35CrossRefPubMed

34.

McGee-Lawrence ME, Li X, Bledsoe KL, Wu H, Hawse JR, Subramaniam M, Razidlo DF, Stensgard BA, Stein GS, van Wijnen AJ, Lian JB, Hsu W, Westendorf JJ (2013) Runx2 protein repress Axin2 expression in osteoblasts and is required for cranio synostosis in Axin2-deficient mice. J Biol Chem 288:5291–302PubMedCentralCrossRefPubMed

35.

Derynck R, Miyazono K (2008) The TGF-β Family. Cold Spring Harbor, New York

36.

Komori T (2006) Regulation of osteoblast differentiation by transcription factors. J Cell Biochem 99:1233–9CrossRefPubMed

37.

Mendelson A, Frank E, Allred C, Jones E, Chen M, Zhao W, Mao JJ (2011) Chondrogenesis by chemotactic homing of synovium, bone marrow, and adipose stem cells in vitro. FASEB J 25:3496–504PubMedCentralCrossRefPubMed

38.

Bar I, Zilberman Y, Zeira E, Galun E, Honigman A, Turgeman G, Clemens T, Gazit Z, Gazit D (2003) Molecular imaging of the skeleton: quantitative real-time bioluminescence monitoring gene expression in bone repair and development. J Bone Miner Res 18:570–8CrossRef

39.

Cantatore FP, Corrado A, Grano M, Quarta L, Colucci S, Melillo N (2004) Osteocalcin synthesis by human osteoblasts from normal and osteoarthritic bone after vitamin D3 stimulation. Clin Rheumatol 23:490–5CrossRefPubMed

40.

Fernandes KR, Ribeiro DA, Rodrigues NC, Tim C, Santos AA, Parizotto NA, De Araujo HS, Driusso P, Rennó AC (2013) Effects of low-level laser therapy on the expression of osteogenic genes related in the initial stages of bone defects in rats. J Biomed Opt 3:038002CrossRef

41.

Stein E, Koehn J, Sutter W, Wendtlandt G, Wanschitz F, Thurnher D, Baghestanian M, Turhani D (2008) Initial effects of low-level laser therapy on growth and differentiation of human osteoblast-like cells. Wien Klin Wochenschr 120:112–7CrossRefPubMed

42.

Marie PJ (2003) Fibroblast growth factor signaling controlling osteoblast differentiation. Gene 316:23–32CrossRefPubMed

43.

Boden SD (2005) The ABCs of BMPs. Orthop Nurs 24:49–52CrossRefPubMed

44.

Saygun I, Nizam N, Ural AU, Serdar MA, Avcu F, Tözüm TF (2012) Low-level laser irradiation affects the release of basic fibroblast growth factor (bFGF), insulin-like growth factor-I (IGF-I), and receptor of IGF-I (IGFBP3) from osteoblasts. Photomed Laser Surg 30:149–54CrossRefPubMed

45.

Du X, Xie Y, Xian CJ, Chen L (2012) Role of FGFs/FGFRs in skeletal development and bone regeneration. J Cell Physiol 227:3731–43CrossRefPubMed

46.

Batista JD, Sargenti-Neto S, Dechichi P, Rocha FS, Pagnoncelli RM (2015) Low-level laser therapy on bone repair: is there any effect outside the irradiated field? Lasers Med Sci. 2015 May 15. [Epub ahead of print]

Titel: Effects of low-level laser therapy on the expression of osteogenic genes during the initial stages of bone healing in rats: a microarray analysis
verfasst von: Carla Roberta Tim
Paulo Sérgio Bossini
Hueliton Wilian Kido
Iran Malavazi
Marcia Regina von Zeska Kress
Marcelo Falsarella Carazzolle
Nivaldo Antonio Parizotto
Ana Cláudia Rennó
Publikationsdatum: 01.12.2015
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 9/2015
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-015-1807-5

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Effects of low-level laser therapy on the expression of osteogenic genes during the initial stages of bone healing in rats: a microarray analysis

Abstract

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

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