Renal malignant tumor is one of the common tumors of the urinary system, and its incidence accounts for 3% of adult malignant tumors. The incidence of renal cell carcinoma(RCC) is lower than that of prostate and bladder cancer, accounting for the third most common urinary system tumor [
1,
2]. Renal cell carcinoma(RCC) is a malignant tumor arising from the tubular epithelium, accounting for 80–90% of renal malignancies [
3]. The causes of RCC are not fully clarified and may be related to genetics, smoking, obesity, hormone levels, hypertension and antihypertensive drugs, diet and occupational environment [
4]. Clear cell renal cell carcinoma (ccRCC) is the most common pathological type (80%) [
5], followed by papillary renal cell carcinoma (10% ~ 15%) [
6] and chromophobe cell carcinoma (5%) [
7]. For early RCC without metastasis, surgical resection of the primary tumor is still the most effective treatment [
8]. However, approximately 30% of patients with RCC already present with distant metastasis at presentation. Meanwhile, 20–40% of patients with localized RCC will develop distant metastasis after surgery [
9]. The biological behavior of RCC is also complex and diverse, and invasion of the venous system is one of its unique biological behaviors [
10], and the incidence of venous tumor thrombus accounts for 5–15% of RCC, with renal venous tumor thrombus being the most common, accounting for 60–78% of venous tumor thrombus [
11]. Renal vein tumor thrombus can further progress to the vein or even the right atrium. Because renal cell carcinoma is insensitive to traditional chemoradiotherapy, molecular targeted drugs and immunotherapy are commonly used treatments for unresectable advanced RCC [
12,
13]. Commonly used molecular targeted drugs for the treatment of RCC are sunitinib, sorafenib, and pazopanib, which are multi-targeted tyrosine kinase inhibitors. However, targeted drugs often experience drug resistance and disease progression after a period of use, affecting the survival of patients. In recent years, immune checkpoint inhibitors have also been applied in the treatment of advanced renal cell carcinoma, and a number of randomized controlled studies have been conducted, obtaining positive findings. In the phase 3 CheckMate 214 trial [
14], nivolumab plus ipilimumab led to improved efficacy outcomes versus sunitinib in both intermediate-risk/poor-risk patients that were maintained through 42 months’ minimum follow-up, with manageable safety. Nirmish Singla et al. [
15] reported that cytoreductive nephrectomy combined with immunotherapy had a longer OS than immunotherapy alone for metastatic renal cell carcinoma (mOS NR vs. 11.6 months; hazard ratio 0.23, P < 0.001). Transarterial chemoembolization (TACE) was first proposed by Yamada in 1978 and is a commonly used treatment for advanced hepatocellular carcinoma [
16]. The main principle of TACE is the transcatheter injection of chemotherapeutic agents and embolic agents into the tumor tissue. On the one hand, the cytotoxicity of chemotherapeutic drugs can induce the apoptosis of tumor cells and inhibit the proliferation of tumor cells; on the other hand, after embolization of tumor vessels, tumor tissue is ischemic and hypoxic and necrotic. In recent years, with the continuous update of interventional devices, embolization materials and operation techniques, TACE is also used for the treatment of multiple solid tumors throughout the body. There have been previous reports on preoperative combined TAE for RCC, and TAE has clinical significance for reducing the occurrence of complications such as intraoperative bleeding during surgery [
17]. As one of the common solid tumors, some scholars have also tried TACE for RCC [
18]. The aim of this study was to explore the efficacy and safety of TACE combined with sunitinib vs. sunitinib in the treatment of unresectable advanced renal cell carcinoma.
Discussion
About 70% of patients present with localized renal cell carcinoma(RCC) or locally advanced RCC, but about 30% of these patients experience recurrence or metastasis within 3 years after surgery [
20]. At present, systemic drug therapy is the main treatment for Metastatic renal cell carcinoma (mRCC), including targeted drug therapy and immunotherapy [
21].Before the advent of molecular targeted drugs, biological immunotherapy with interleukin-2 (IL-2) and interferon-α (IFN-α) was mainly used for advanced RCC [
22], with an objective response rate of less than 30%, an mPFS of only 5 months [
23], and a five-year survival rate of 8% for patients with mRCC [
24]. With the application of a variety of targeted drugs in the treatment of RCC, the prognosis of patients with advanced RCC has improved significantly. According to the mechanism of action, targeted drugs are mainly divided into three types: upstream inhibitors targeting the mTOR pathway in tumors [
25], such as Everolimus, Sirolimus, etc.; intermediate monoclonal antibodies targeting VEGF-A secreted by tumor cells, such as Bevacizumab, etc. [
26]; downstream tyrosine kinase inhibitors targeting VEGFR/PDGFR and other receptors on vascular endothelial cells [
27], such as Sorafenib, Sunitinib, Pazopanib, etc. Sunitinib is a multi-target receptor tyrosine kinase inhibitor [
28], with the main targets of vascular endothelial growth factor receptor 1–2 (VEGFR1-2), platelet-derived growth factor receptor (PDGFR-α, PDGFR-β), stem cell growth factor receptor (c-KIT) and FMS-like tyrosine kinase 3 (FLT-3). It has the effects of anti-tumor angiogenesis and inhibiting tumor cell proliferation, thereby inhibiting the occurrence and metastasis of tumors. It is one of the most commonly used first-line drugs for the treatment of RCC at present. Michael Moran et al. reported [
29] that in both randomized controlled trials(RCTs) and Real-World Data(RWD), sunitinib was an effective first-line treatment strategy for mRCC, with a mPFS of 7.5–11.0 months in RWD and 5.6–15.1 months in RCTs reported in the literature. The ORR was 14.0-34.6% in RWD and 18.8–46.9% in RCTs. The mOS was 6.8–33.2 months in RWD and 21.8–31.5 months in RCTs. Xiu-Lan Liu et al. reported in the study [
30] that the treatment of RCC with sunitinib had better efficacy and safety than sorafenib. Our findings were an ORR of 39.2% and a DCR of 66.7% in the sunitinib group. In the sunitinib group, mPFS was 10.9 months (95% CI 8.9–11.1) and mOS was 25.7 months (95% CI 23.6–27.8). This result is consistent with other studies that treatment with sunitinib improves survival in patients with advanced RCC.
TACE is currently one of the commonly used means for the treatment of various solid tumors throughout the body. TACE has been used in hepatocellular carcinoma for about forty years. With the improvement of interventional techniques and the development of interventional devices (including embolic agents), TACE is also more and more widely used in the treatment of RCC [
31]. For patients with pain, hematuria or tumor rupture and hemorrhage, TACE can effectively relieve patients’ clinical symptoms and play a role in hemostasis. Bryan Wright et al reported [
32] that transarterial embolization (TAE) can effectively improve the symptoms of patients with RCC, such as pain and hematuria, and is a safe treatment strategy. 60 patients treated for pain and hematuria were reported in the study, with improvement in pain in 59 patients (98.3%) and improvement in hematuria in 57 patients (95%) after TAE. For advanced RCC that cannot be surgically removed, TACE can effectively and rapidly reduce the tumor burden [
33]. The injection of chemotherapeutic drugs and embolic agents into the tumor site through the catheter has a variety of advantages [
34]: (1) the total use of chemotherapeutic drugs is less than that of systemic chemotherapy, and the incidence of chemotherapy-related toxicities is low; (2) the concentration of chemotherapeutic drugs in the tumor site is high, which can better eliminate tumor cells; (3) the combined use of embolic agents by chemotherapeutic drug-lipiodol emulsion can slow down the loss of chemotherapeutic drugs, the local chemotherapeutic drugs are slowly released, and the effective drug concentration can be maintained in the tumor site for a long time; (4) after embolization of the tumor feeding artery, the ischemic necrosis of tumor tissue is more obvious, which can effectively reduce the tumor burden in a short time. For renal artery chemoembolization, there are also corresponding operating specifications [
35]. Referring to the chemotherapy regimen for renal cancer, doxorubicin is one of the commonly used drugs [
36] and the most classically used chemotherapeutic drug for TACE for liver cancer. The Nathaniel R’ study [
37] suggested that the combination of doxorubicin with epigenetic therapeutics can be beneficial in clinical treatment of renal cancer patients with wild-type VHL and p53. Therefore, the chemotherapeutic drug used in chemoembolization of RCC in our center is doxorubicin. J H Park et al reported[
38] that for patients with inoperable RCC, TAE using lipiodol + ethanol emulsion was an effective and safe treatment. Noor Riza Perdana et al reported [
39] that RAE is an effective treatment for large unresectable renal tumors and can reduce mortality. T Kato et al reported [
40] that chemoembolization with mitomycin C microcapsules is a very effective treatment for renal cell carcinoma. It is reported by H Saitoh et al [
41] that the use of renal artery embolization in the treatment of advanced RCC is an effective translational treatment, which can reduce the tumor burden, increase the chance of surgery for patients, and improve the survival of patients. A M Granov et al reported [
42] that the use of chemoembolization for advanced RCC has a higher 2-year and 3-year survival rate relative to embolization alone. The results of this study showed that in TACE + Sunitinib group, CR in 4 patients (8.5%), PR in 27 patients (57.5%), SD in 9 patients (19.1%) and PD in 7 patients (14.9%); ORR was 66.0% and DCR was 85.1%. TACE is similar to surgical cytoreductive surgery in reducing the tumor burden of RCC, but TACE is more minimally invasive and patients experience less pain and trauma.
Numerous studies have reported [
43] that tumor tissue hypoxia leads to a significant increase in VEGF levels in patients with hepatocellular carcinoma(HCC) after receiving TACE therapy. Similar to HCC, tumor tissue ischemia and hypoxia in patients with RCC induce increased hypoxia-inducible factor(HIF) activity after TACE, which in turn leads to overexpression of various tumor-promoting factors such as vascular endothelial growth factor (VEGF), transforming growth factor (TGF), and platelet-derived growth factor (PDGF) [
18]. The overexpression of these cytokines leads to cell proliferation, apoptosis inhibition, angiogenesis, and increased adhesion and mobility, which in turn leads to tumor development [
1,
44,
45]. Molecular targeted drugs can target and inhibit these cytokine receptors, which can compensate for the lack after TACE treatment in a mechanistic manner, thereby improving the effect of TACE treatment. TACE combined with molecular targeted drug therapy has been very explored in the treatment of HCC, and there are also a large number of literatures reporting that combination therapy can effectively improve the deficiencies of TACE [
46]. Masatoshi Kudo study reported [
47] that TACE plus sorafenib significantly improved PFS over TACE alone in patients with unresectable HCC. It is reported by Zhigang Fu et al[
48] that Combination treatment with TACE and lenvatinib may improve clinical outcomes over TACE monotherapy with a manageable safety profile for unresectable HCC. Although TACE for RCC has not been reported in combination therapy. However, based on the experience of TACE combined with molecular targeted drugs in the treatment of HCC, our center also uses molecular targeted drug Sunitinib in patients with advanced RCC after TACE. This study found that in the TACE + sunitinib group, mPFS was 15.6 months (95% CI 14.9–17.1); mOS was 35.0 months (95% CI 32.7–37.4). PFS and OS were significantly longer in the TACE + sunitinib group than in the sunitinib-only group, and patients had a better survival benefit.
The current guidelines recommend a dose of 50 mg qd for sunitinib, 4/2 regimen, that is 4 weeks of treatment followed by 2 weeks of rest as a cycle. Some scholars have also proposed a 2/1 regimen after research, that is treatment with 50 mg qd for 2 weeks, followed by 1 week of rest. Some patients may experience AEs, such as hand-foot syndrome, fatigue, leukopenia, hypertension, thrombocytopenia, and anemia. S Bracarda et al. reported [
49] that patients treated with 2/1 regimen had similar efficacy, but the incidence of side effects was significantly reduced. In this study, 50 mg qd(4/2 regimen) was used for sulitinib. There was no statistical difference in the incidence rate of fatigue, anorexia, hypertension, hand-foot syndrome, diarrhea and rash between the two groups (P > 0.05), and the incidence rate of grade 3–4 AEs was very low in the two groups. However, the incidence of abdominal pain, fever, and vomiting was significantly higher in the TACE + sunitinib group than in the sunitinib group (abdominal pain: 55.3% vs. 13.7%; fever: 61.7% vs. 7.8%; vomiting: 40.4% vs. 19.6%; P < 0.05). The main reason was the occurrence of post-embolization syndrome after TACE in the combined treatment group. T Onishi et al. reported [
50] that the most important AEs after TAE for unresectable advanced RCC were abdominal pain, fever, nausea and vomiting, and all patients in the study recovered from the AEs. Most post-embolization syndromes can be relieved in a short time after symptomatic treatment. Yonghua Bi et al. [
51] reported that 35 patients with unresectable RCC treated with TACE developed fever, abdominal pain, nausea and vomiting, and these symptoms were relieved after 2–3 days. It is reported by Shu-Kui Qin et al. [
52] that in the first-line treatment of mRCC with sunitinib, the most common AEs in the Chinese population were hand-foot syndrome (63.8%), leukopenia (52.4%), fatigue (51.4%) and thrombocytopenia (51.4%), all of which were tolerable, and AEs predicted longer PFS and OS. There was no statistical difference in ECOG score after treatment between the two groups in this study (P > 0.05). After treatment, total bilirubin, BUN and Cr in the two groups were significantly higher than those before treatment. After treatment, GFR, WBC and PLT in the two groups were lower than those before treatment, but there was no statistical difference between the two groups (P > 0.05). In TACE + Sunitinib group, WBC increased after treatment compared with that before treatment, which was considered to be ischemic necrosis of tumor tissue after TACE, and aseptic inflammation caused by necrotic tissue, thus WBC increased compared with that before treatment. While in Sunitinib group, WBC decreased after treatment compared with that before treatment, which might be considered to be AEs caused by the drug. Albumin decreased after TACE + sunitinib group and sunitinib group compared with that before treatment, and decreased more in sunitinib group, with statistical difference (P < 0.05). After analysis, 14 patients (27.5%) had SD and 17 (33.3%) had PD in Sunitinib group. Their disease control rate was worse than TACE + Sunitinib group, which might be associated with decreased liver function due to disease progression. The results of this study showed that the combination therapy did not increase the risk of other treatment-related AEs except that the incidence of post-embolization syndrome which was higher in the TACE + sunitinib group than in the sunitinib group.
The shortcomings of this study are that the data are from a single center, and it is a retrospective study with limited sample size. A multicenter, large-sample, prospective study is feasible at a later stage to provide more help for clinical work.
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