Background
Breast cancer is a heterogeneous disease that has several clinical, histological, and genetic forms. There have been many attempts to categorize this heterogeneous disease, and now molecular classification into five groups (luminal A, luminal B, HER-2, normal breast-like, and basal-like) can be accomplished by gene-expression profiling [
1,
2].
Among these molecular subtypes, basal-like breast cancer has a more aggressive clinical course than other subtypes and is commonly known as triple-negative breast cancer (TNBC), which is defined clinically as lacking estrogen receptor (ER) and progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2). TNBC accounts for 10–17 % of all breast cancers [
3‐
8]. Highly heterogeneous and known to have several molecular subtypes, TNBC is difficult to treat because it does not respond to hormonal or targeted therapy such as Herceptin, except chemotherapy [
9‐
11].
Programmed death 1 (PD-1) is a check point molecule in immune reactions and can be expressed in various immune cells [
12]. PD-L1, a ligand of PD-1, is expressed in cancer cells and the binding of PD-L1 with PD-1 helps cancer cells avoid antitumor immune responses [
13,
14]. PD-L1 expression has been reported at rates of 20–70 % in lung cancer [
13,
15‐
18], urinary bladder cancer [
19], malignant melanoma [
20], and ovarian cancer [
21]. PD-L1 was expressed in not only tumor cells but immune cells in previous studies of breast cancer [
22‐
24], and lung cancer [
25]. In addition, its expression in immune cells demonstrated clinical implication [
23]. Therefore, the evaluation of PD-L1 should be performed in both cancer cell and immune cells.
Target therapy for PD-L1 in PD-L1-expressing cancers represents a possible treatment for inducing antitumor immune responses. PD-L1-targeted therapy has been investigated in preclinical and clinical trials in many tumors [
15‐
17,
19,
26‐
28] and anti-PD-L1 antibodies such as BMS-936,559 [
29] and MPDL3280A [
16,
19] have been developed. Knowledge of the expression of PD-L1 in cancer cells plays an important role in tailored therapy planning, and is easily evaluated using immunohistochemistry (IHC) with a monoclonal PD-L1 antibody in formalin-fixed and paraffin-embedded (FFPE) specimens. Multiple monoclonal PD-L1 antibodies such clone 28-8 [
30], clone 22C3 [
31], clone SP142 [
16,
19], and clone E1L3N [
32,
33] have been commercially developed.
The aim of this study was to evaluate the expression of PD-L1 using different immunohistochemical antibodies in TNBC and associated clinical implications.
Methods
Patient selection
We analyzed 218 patients with TNBC who underwent surgery at Severance Hospital between January 2000 and December 2006. This study was approved by the Institutional Review Board of Severance Hospital. All patients were diagnosed as having invasive ductal carcinoma, not otherwise specified (NOS) by pathologists. We defined TNBC as when IHC for ER, PR, and HER-2 and FISH for HER-2 were all negative.
ER and PR immunohistochemistry signal were considered positive when more than 1 % of invasive tumor cells were expressed [
34]. HER-2 staining was scored according to the American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) guideline using the following categories: 0, no immunostaining; 1+, weak incomplete membranous staining in any proportion of tumor cells; 2+, complete membranous staining, either non uniform or weak in at least 10 % of tumor cells; and 3+, uniform intense membranous staining in >30 % of tumor cells [
35]. Cases with 0 to 1+ were regarded as negative and case with 3+ was considered as positive. Cases with HER-2 2+ were investigated with FISH (Vysis pathvision HER-2 kit) for HER-2 gene status. As proposed by the ASCO/CAP guideline, an absolute HER-2 gene copy number lower than four or HER-2 gene/chromosome 17 copy number ratio (HER-2/Chr17 ratio) of less than 1.8 was considered HER-2 negative; an absolute HER-2 copy number between 4 and 6 or HER-2/Chr17 ratio between 1.8 and 2.2 was considered HER-2 equivocal; and an absolute HER2 copy number greater than 6 or HER-2/Chr17 ratio higher than 2.2 was considered HER-2 positive.
Formalin-fixed and paraffin-embedded tissue specimens from 218 cases of primary breast cancer were included. All archival hematoxylin and eosin (H&E)-stained slides for each case were reviewed by one pathologists (Koo JS). The histological grade was accessed using Nottingham grading system [
36]. According to the microscopic cancer stoma findings, TNBC was categorized as follows [
37]:
desmoplastic type, with cellular fibroblast/myofibroblast proliferation as the main stromal content;
sclerotic type, which has a small cell component and fibrotic collagenous component as its main stromal content; or
inflammatory type, with stroma mainly composed of inflammatory cells such as lymphocytes. Tumor staging was based on the 8th American Joint Committee on Cancer (AJCC) criteria. Disease-free survival (DFS) time was measured from the date of the first curative surgery to the date of the first locoregional or systemic relapse, or death without any type of relapse. Overall survival (OS) time was calculated from the date of the first curative operation to the date of the last follow-up or death from any cause. Histologic parameters were evaluated from the H&E-stained slides. Clinical parameters evaluated in each tumor included patient age at initial diagnosis, lymph node status, local recurrence, systemic recurrence, and patient’s survival.
Tissue microarray
On H&E-stained slides of tumors, a representative area was selected and a corresponding spot was marked on the surface of the paraffin block. Using a punch machine, the selected area was punched out and a 3-mm tissue core was placed into a 6 × 5 recipient block. More than two tissue cores were extracted to minimize extraction bias. Each tissue core was assigned with a unique tissue microarray location number that was linked to a database containing other clinicopathologic data.
Immunohistochemistry (IHC)
The antibodies and dilution used for IHC are shown in Additional file
1: Table S1. All immunohistochemistry was performed with formalin-fixed, paraffin-embedded tissue sections using an automatic immunohistochemistry staining device (Benchmark XT, Ventana Medical System, Tucson, AZ, USA). Briefly, 5-µm-thick formaldehyde fixed paraffin-embedded tissue sections were transferred onto adhesive slides and dried at 62 °C for 30 min. Standard heat epitope retrieval was performed for 30 min in ethylene diamine tetraacetic acid, pH 8.0, in the autostainer. The samples were then incubated with primary antibodies. After incubation with primary antibodies, The sections were subsequently incubated with biotinylated anti-mouse immunoglobulins, peroxidase-labeled streptavidin (LSAB kit, DakoCytomation), and 3,30-diaminobenzidine. Negative control samples were processed without the primary antibody. Slides were counterstained with Harris hematoxylin. Positive control tissue was used as per the manufacturer’s recommendation (placenta and tonsil). Slides were counterstained with Harris hematoxylin. Optimal primary antibody incubation times and concentrations were determined by serial dilution for each immunohistochemical assay using a tissue block fixed and embedded exactly as for the experiments.
Interpretation of immunohistochemical staining
We evaluated the expression of immunohistochemical markers in cancer cells and peri-tumoral immune cells by light microscopy. Expression in cancer cells was analyzed by various cut-off values (1, 5, 10 and 50 %) and expression in immune cells was analyzed as follows: negative, no immunostaining; low-positive, stained immune cells ≤30/high power field (HPF); and high-positive, stained immune cells >30/HPF.
Interpretation of immunohistochemical staining was performed independently by three researchers (SWY, LYK, KJS). We investigated the proportion of expression (%) in cancer cells according to the above criteria, counted the number of expressed immune cells. Each of three pathologists analyzed the samples and reached a conclusion, which were then analyzed to determine inter-reader concordance. The final results were determined through discussion and multi-view microscopy in the cases that showed discrepancy among the three pathologists.
Statistical analysis
Data were analyzed using SPSS for Windows, Version 12.0 (SPSS Inc., Chicago, IL, USA). For determination of statistical significance, Student’s t and Fisher’s exact tests were used for continuous and categorical variables, respectively. The inter-reader concordance rate and concordance rate among PD-L1 antibodies were analyzed using the Kappa-Cohen method. Statistical significance was when p < 0.05. Kaplan–Meier survival curves and log-rank statistics were employed to evaluate time to tumor recurrence and overall survival. Multivariate regression analysis was performed using Cox proportional hazards model.
Discussion
This study sought to evaluate the immunohistochemical expression of PD-L1 using several monoclonal antibodies in TNBC. We performed IHC under the same conditions with different antibody clones and found differences in PD-L1 expression in both tumor cells and immune cells (Table
7).
Table 7
Multivariate analysis of triple negative breast cancer survival
Histologic grade | | | 0.560 | | | 0.204 |
I/II versus III | 0.725 | 0.246–2.136 | | 0.537 | 0.206–1.402 | |
T stage | | |
0.027
| | |
0.016
|
T1 versus T2/T3 | 10.21 | 1.306–79.90 | | 6.317 | 1.413–28.23 | |
Lymph node metastasis | | |
0.019
| | |
0.013
|
No versus Yes | 3.918 | 1.254–12.24 | | 3.564 | 1.304–9.740 | |
Ki-67 LI | | | 0.327 | | | 0.711 |
≤14 versus >14 | 0.581 | 0.197–1.719 | | 1.209 | 0.443–3.298 | |
Stromal type | | | 0.988 | | | 0.699 |
Desmoplastic/sclerotic versus inflammatory | 0.990 | 0.266–19.15 | | 1.287 | 0.359–4.613 | |
PD-L1 (28-8) (I) | | | 0.081 | | |
0.036
|
Negative versus positive | 4.019 | 0.843–19.15 | | 5.112 | 1.110–23.54 | |
The high concordance rate among the three readers in the interpretation of PD-L1 yielded acceptable inter-reader reproducibility with a kappa value >0.610 in both cancer cells and immune cells. Especially in cancer cells, the concordance rate was highest at a 1 % cut-off value and lowest at a 10 % cut-off value. This is likely because the reader tends to interpret as positive in 1 % cut-off even in slight expression and increase concordance rate, and tends to have more likely subjective interpretation in 10 % cut-off value. However, improvement in the response rate from 35 % at a 1 % cut-off value to 44 % at a 5 % cut-off value was seen in a nivolumab phase I study targeting malignant melanoma, indicating that the higher 5 % cut-off value was more reasonable [
38]. Therefore, further response-based studies of TNBC are necessary.
Variation in PD-L1 expression rates previously observed in a study of various cancers has been attributed to differences in cut-off values, antibodies, and study populations [
39,
40], and this phenomenon is also commonly seen with other antibodies [
41]. In lung cancer characterized with PD-L1 expression, expression rates varied from 24 to 60 % using the same 5 % cut-off value [
15,
16,
42], and from 21 to 95 % according to different cut-off values of 1, 10 and 50 % [
13,
17,
18,
43].
PD-L1 expression in TNBC was present at 19 % (clone 5H1 with a 5 % cut-off) [
44] and 64–80 % (clone E1L3N, with a 1 % cut-off) [
45] in previous studies. In our study, the expression rate was 11.5–14.7 % with a 1 % cut-off value and 11.0–14.7 % with a 5 % cut-off value, which was similar to the previous report of 19 %. We presume that differences in expression rate are due primarily to different characteristics of the PD-L1 antibody clones, because all samples were stained using the same immunohistochemical conditions.
PD-L1 showed membranous expression in this study, in agreement with one previous study in TNBC [
44], but the other TNBC study reported both cytoplasmic and membranous expression of PD-L1 [
45]. PD-L1 expression has been reported in the cell membrane [
17,
26‐
28] or membrane and cytoplasm [
13,
16,
18] in other cancers. We performed IHC using an automatic IHC staining device, but the previous research in TNBC that evaluated cytoplasm expression had no clear description of staining, making it difficult to compare our findings directly with previous data [
45]. We found expression of PD-L1 in 19.3–37.6 % of cancer cells and immune cells, depending on the antibody clone. Although our expression rate was different than a previous study with 93 % immune cell expression in TNBC, it was in concordance with the previous report in terms of positive immune cell expression [
45]. PD-L1 expression in immune cells has been reported in other cancers [
16,
19,
25,
28]. There are differences between oncogene-driven PD-L1 expression and inflammation-driven PD-L1 expression. While oncogene-driven PD-L1 expression is constitutive and diffuse, inflammation-driven PD-L1 expression is limited to sites of IFNγ-mediated immunologic attack [
46]. In addition, inflammation-driven PD-L1 expression is related to immune infiltrates, while oncogene-driven PD-L1 expression is not [
28,
47]. Thus, further study to know which expression between two expressions is more related to the expression of PD-L1 on immune cell in TNBC.
In this study, the concordance rate between PD-L1 (28-8) and PD-L1 (E1L3N) was high in both cancer cells and immune cells while PD-L1 (SP142) showed low concordance rates with the other two antibodies. Previous studies reported poor concordance (kappa value: 0.124–0.340) between PD-L1 (E1L3N) and PD-L1 (SP142) in lung cancer [
48], but a high concordance rate between PD-L1 (E1L3N) and PD-L1 (SP142) (more than 85 %) in malignant melanoma [
49]. Therefore, concordance seems to vary according to cancer type; further study on this topic is needed.
PD-L1 positivity in immune cells (28-8 clone) was an independent prognostic factor in our study, in contrast to previous studies showing good prognosis [
50‐
53] or poor prognosis [
54,
55] with PD-L1 expression in cancer cells. Since the prognostic and predictive significance of PD-L1 expression in immune cells such as macrophages and lymphocytes was not associated with tumor-related PD-L1 expression [
16], further study of the biologic implications of PD-L1 in immune cells during TNBC is needed.
In breast cancer, tumor infiltrating lymphocytes (TILs) have been linked with good prognosis [
56], as have stromal TIL [
57]. Therefore, tumor immunity may relate to prognosis in TNBC. PD-L1 expression was significantly higher in inflammatory type cancer cells and immune cells with all tested antibodies. Therefore, we assume that PD-L1 expression in stromal immune cells is related to prognosis, and further research is needed to better elucidate this relationship.
Clinical trials targeting PD-L1 are underway [
17,
58‐
60] and good responses have been reported [
61,
62]. In TNBC, which has no effective therapy, PD-L1-targeting agents may play an important role and strong biomarkers that can predict treatment response are needed. Accordingly, staining of PD-L1 monoclonal antibodies may act as a biomarker for PD-L1-targeting agents, but clinical trials evaluating TNBC response to PD-L1-targeting agents using monoclonal antibody staining will be needed to validate this strategy.
The limitation of this study was the potential difference in results between TMA and the whole cancer tissue section. Breast cancer also shows intracancer heterogeneity, like other types of cancer, and the expression of PD-L1 may cause differences between TMA and the whole cancer tissue section. PD-L1 expression in lung cancer showed a high discordance rate between TMA samples and whole tissue sections [
48]. In a previous study, the results of immunohistochemical staining of ER in breast cancer were different in 5.5 % between TMA and the whole cancer tissue section. Furthermore, when the number of cores was greater than one, this difference decreased to 1.4 % [
63]. In this study, two core extractions per case were performed to reduce this bias.
Authors’ contributions
WYS participated in the design of the study and performed the statistical analysis and carried out the immunoassays. YL participated in its design. JSK conceived the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.