Introduction
Materials and methods
Virus, seedlings, and inoculation
RNA extraction and cDNA synthesis
Selection of cucumber miRNAs in response to CGMMV infection
Prediction and analysis of target genes and construction of a regulatory network
RT-qPCR analysis of miRNAs and their putative target genes
Results
Selection of cucumber miRNAs in response to CGMMV infection
Prediction of target genes and construction of a regulatory network
miRNA | Target accession | Inhibition type | Target | Putative function |
---|---|---|---|---|
miR159 | Csa4M022940.1 | Cleavage | Transcription factor MYB29-like | Abiotic stress tolerance and biotic stress resistance |
miR169 | Csa4M015840.1 | Translation | Putative disease resistance protein RGA3-like | Disease resistance that triggers a defense system that restricts pathogen growth |
miR172 | Csa5M175970.1 | Cleavage | Ethylene-responsive transcription factor RAP2-7-like | May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways |
miR838 | Csa6M520360.1 | Cleavage | MACPF(membrane attack complex and perforin) domain-containing protein CAD1-like | CAD1 protein negatively controls the SA-mediated pathway of programmed cell death during plant immunity responses. |
miR854 | Csa2M224260.1 | Cleavage | Probable WRKY transcription factor 21-like | Defense responses |
miR5658 | Csa1M470280.1 | Cleavage | LRR receptor-like serine/threonine-protein kinase FLS2-like | Disease resistance |
csa-miRn1-3p | Csa7M073450.1 | Cleavage | UDP-glycosyltransferase 73B3-like | Pathogen-responsive gene |
csa-miRn5-5p | Csa1M616240.1 | Cleavage | Glucan endo-1,3-beta-glucosidase, basic isoform-like | Plant defense-related product targeting fungal pathogens |
csa-miRn6-3p | Csa1M424880.1 | Cleavage | Pathogenesis-related homeodomain protein-like | Defense response |
Csa3M146460.1 | Cleavage | Vicilin-like antimicrobial peptides 2-1-like | Defense response to bacteria and fungi resulting in the death of the foreign organism | |
Csa5M139630.1 | Cleavage | Ethylene-responsive transcription factor CRF4-like | May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways | |
Csa7M425940.1 | Cleavage | Putative disease resistance protein RGA4-like | Disease resistance that triggers a defense system that restricts pathogen growth | |
Csa7M374620.1 | Cleavage | Linoleate 9S-lipoxygenase 1-like | Influence on plant physiology including growth and development, pest resistance, and senescence or responses to wounding. | |
Csa6M091910.1 | Cleavage | RNA polymerase II C-terminal domain phosphatase-like 3-like | Modulates plant growth, stress (cold), and phytohormones responses | |
Csa4M045040.1 | Cleavage | Probable dehydrin LEA-like | Response to stress and water | |
Csa6M133770.1 | Cleavage | Ethylene-responsive transcription factor CRF4-like | May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways |
Differential expression of cucumber miRNAs during CGMMV infection
Differential expression of target genes during CGMMV infection
Correlation between miRNA and target gene expression
miRNA/ target | Leaf | Stem | Root | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 7 | 14 | 21 | 28 | 35 | 42 | 1 | 7 | 14 | 21 | 28 | 35 | 42 | 1 | 7 | 14 | 21 | 28 | 35 | 42 | |
miR159/Csa4M022940.1 |
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miR169/Csa4M015840.1 |
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miR172/Csa5M175970.1 |
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miR838/Csa6M520360.1 |
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miR854/Csa2M224260.1 |
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miR5658/Csa1M470280.1 |
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csa-miRn6-3p/Csa7M425940.1 |
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| No data | |||||||||||||
csa-miRn6-3p/Csa4M045040.1 |
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