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07.09.2018 | Research Article

Ferumoxytol Can Be Used for Quantitative Magnetic Particle Imaging of Transplanted Stem Cells

verfasst von: Hossein Nejadnik, Prachi Pandit, Olga Lenkov, Arian Pourmehdi Lahiji, Ketan Yerneni, Heike E. Daldrup-Link

Erschienen in: Molecular Imaging and Biology | Ausgabe 3/2019

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Abstract

Purpose

To evaluate, if clinically translatable ferumoxytol nanoparticles can be used for in vivo detection and quantification of stem cell transplants with magnetic particle imaging (MPI).

Procedures

Mesenchymal stem cells (MSCs) were labeled with ferumoxytol or ferucarbotran and underwent MPI, magnetic resonance imaging (MRI), Prussian blue staining, and inductively coupled plasma (ICP) spectrometry. Unlabeled, ferumoxytol, and ferucarbotran-labeled MSCs were implanted in calvarial defects of eight mice and underwent MPI, MRI, and histopathology. The iron concentration calculated according to the MPI signal intensity and T2 relaxation times of the three different groups were compared using an analysis of variance (ANOVA) with Bonferroni correction, and a p < 0.05.

Results

Compared to unlabeled controls, ferumoxytol- and ferucarbotran-labeled MSC showed significantly increased iron content, MPI signal and MRI signal. The ferumoxytol MPI signal was approximately 4× weaker compared to ferucarbotran at equimolar concentrations (p = 0.0003) and approximately 1.5× weaker for labeled cells when using optimized labeling protocols (p = 0.002). In vivo, the MPI signal of ferumoxytol-labeled MSC decreased significantly between day 1 and day 14 (p = 0.0124). This was confirmed by histopathology where we observed a decrease in Prussian blue stain of MSCs at the transplant site. The MRI signal of the same transplants did not change significantly during this observation period (p = 0.93).

Conclusion

Ferumoxytol nanoparticles can be used for in vivo detection of stem cell transplants with MPI and provide quantitative information not attainable with MRI.

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Titel: Ferumoxytol Can Be Used for Quantitative Magnetic Particle Imaging of Transplanted Stem Cells
verfasst von: Hossein Nejadnik
Prachi Pandit
Olga Lenkov
Arian Pourmehdi Lahiji
Ketan Yerneni
Heike E. Daldrup-Link
Publikationsdatum: 07.09.2018
Verlag: Springer International Publishing
Erschienen in: Molecular Imaging and Biology / Ausgabe 3/2019
Print ISSN: 1536-1632
Elektronische ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-018-1276-x

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Ferumoxytol Can Be Used for Quantitative Magnetic Particle Imaging of Transplanted Stem Cells