The online version of this article (doi:10.1186/1475-2875-11-298) contains supplementary material, which is available to authorized users.
Authors declare that they have no any competing interests.
NK, JPNS, RK, BS performed experimental work, data analysis and manuscript writing. VP and NM gave constructive advice and review the manuscript. NV and AA were involved in all stages of this study. All authors have read and approved the final version of manuscript.
Rapid diagnostic tests (RDTs) have revolutionized the diagnosis of malaria. Among the various factors affecting RDTs sensitivity is genetic variation of the antigen used. The genetic variation in PfHRP2 and PfHRP3 proteins was studied among the Indian Plasmodium falciparum isolates.
One hundred and forty isolates of P. falciparum were collected from six geographical regions of India. Target genes encoding PfHRP2 and PfHRP3 antigens were sequenced to study genetic polymorphism. Minimum detection limit giving a positive rapid diagnostic test was also determined.
Extensive variations were observed in amino acid repeat types of PfHRP2 and PfHRP3. PfHRP2 exhibited more polymorphism than PfHRP3. Significant relation was observed between type 2 and type 7 repeats and RDT detection rate as higher number of these repeats showed better sensitivity with RDTs.
The results provide insights into the genetic diversity of Pfhrp2 and Pfhrp3 genes among Indian P. falciparum population and its relation to RDT sensitivity.
Additional file 1: Table S1. Minimum parasite densities among P. falciparum isolates/lines from different regions of India. (DOCX 14 KB)12936_2012_2235_MOESM1_ESM.docx
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- Genetic variation in histidine rich proteins among Indian Plasmodium falciparum population: possible cause of variable sensitivity of malaria rapid diagnostic tests
Jai PN Singh
Anupkumar R Anvikar
- BioMed Central
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