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09.02.2021 | Original Article

In vitro evaluation of physical and chemical parameters involved in aPDT of Aggregatibacter actinomycetemcomitans

verfasst von: Gerdal Roberto de Sousa, Leandro O. Soares, Betânia M. Soares, Rosana de Carvalho Cruz, Paulo Uliana Junior, Thiago Santiago, Luiz M. Farias, Paula Prazeres Magalhães, Lívio B. Silveira, Luciana Almeida Lopes, Marília Wellichan Mancini, Rudolf Huebner, Marcus V. L. Ferreira

Erschienen in: Lasers in Medical Science | Ausgabe 1/2022

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Abstract

Periodontitis is an infectious disease characterized by the destruction of supporting tissues. Antimicrobial photodynamic therapy (aPDT) has been proposed as an improved method for eliminating microorganisms. Its efficiency depends on the correct use of physical and chemical parameters. Thus, these parameters and their relations were evaluated in this study with the purpose of establishing lethal conditions for combating bacterial agents. Diode lasers and light-emitting diodes (LEDs) were characterized to evaluate the absorption profile and resonance of methylene blue (MB) and toluidine blue O (TBO). The relations between light energy density and photosensitizer absorption were determined. Two methodologies were used to evaluate the effects of aPDT against Aggregatibacter actinomycetemcomitans. LED light exhibited a broad emission spectrum with a peak light wavelength of 637 nm and 99% purity. The resonance intensity of MB was higher with diode laser irradiation, and TBO showed higher resonance intensity with LED irradiation. There was no difference in the absorption profile of photosensitizers using diode lasers or LEDs, and variations in power density did not result in an increasing or decrease in light absorption. A. actinomycetemcomitans was susceptible to photodynamic processes. Emission spectra and peak light wavelengths of light sources combined with the absorption profiles of photosensitizers were the main parameters involved in determining the efficiency of photodynamic effects. Power density did not alter the light absorption of photosensitizers. The association between adequate irradiation characteristics and photosensitizer absorption results in complete inactivation of A. actinomycetemcomitans. In addition, the bactericidal effect was not altered by an increase in energy densities.

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Titel: In vitro evaluation of physical and chemical parameters involved in aPDT of Aggregatibacter actinomycetemcomitans
verfasst von: Gerdal Roberto de Sousa
Leandro O. Soares
Betânia M. Soares
Rosana de Carvalho Cruz
Paulo Uliana Junior
Thiago Santiago
Luiz M. Farias
Paula Prazeres Magalhães
Lívio B. Silveira
Luciana Almeida Lopes
Marília Wellichan Mancini
Rudolf Huebner
Marcus V. L. Ferreira
Publikationsdatum: 09.02.2021
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 1/2022
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-021-03267-4

Springer Medizin

Abstract

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