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28.03.2019 | Original Article

Investigating circadian clock gene expression in human tendon biopsies from acute exercise and immobilization studies

verfasst von: Ching-Yan Chloé Yeung, Peter Schjerling, Katja M. Heinemeier, Anders P. Boesen, Kasper Dideriksen, Michael Kjær

Erschienen in: European Journal of Applied Physiology | Ausgabe 6/2019

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Abstract

Purpose

The discovery of musculoskeletal tissues, including muscle, tendons, and cartilage, as peripheral circadian clocks strongly implicates their role in tissue-specific homeostasis. Age-related dampening and misalignment of the tendon circadian rhythm and its outputs may be responsible for the decline in tendon homeostasis. It is unknown which entrainment signals are responsible for the synchronization of the tendon clock to the light–dark cycle.

Methods

We sought to examine any changes in the expression levels of core clock genes (BMAL1, CLOCK, PER2, CRY1, and NR1D1) in healthy human patellar tendon biopsies obtained from three different intervention studies: increased physical activity (leg kicks for 1 h) in young, reduced activity (2 weeks immobilization of one leg) in young, and in old tendons.

Results

The expression level of clock genes in human tendon in vivo was very low and a high variation between individuals was found. We were thus unable to detect any differences in core clock gene expression neither after acute exercise nor immobilization.

Conclusions

We are unable to find evidence for an effect of exercise or immobilization on circadian clock gene expression in human tendon samples.

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Titel: Investigating circadian clock gene expression in human tendon biopsies from acute exercise and immobilization studies
verfasst von: Ching-Yan Chloé Yeung
Peter Schjerling
Katja M. Heinemeier
Anders P. Boesen
Kasper Dideriksen
Michael Kjær
Publikationsdatum: 28.03.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Applied Physiology / Ausgabe 6/2019
Print ISSN: 1439-6319
Elektronische ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-019-04129-2

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Abstract

Purpose

Methods

Results

Conclusions

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