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Archives of Gynecology and Obstetrics

Erschienen in:

01.02.2015 | Gynecologic Endocrinology and Reproductive Medicine

Slow-freezing versus vitrification for human ovarian tissue cryopreservation

verfasst von: Silke Klocke, Nana Bündgen, Frank Köster, Ursula Eichenlaub-Ritter, Georg Griesinger

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 2/2015

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Abstract

Purpose

Ovarian tissue can be cryopreserved prior to chemotherapy using either the slow-freezing or the vitrification method; however, the data on the equality of the procedures are still conflicting. In this study, a comparison of the cryo-damage of human ovarian tissue induced by either vitrification or slow-freezing was performed.

Methods

Ovarian tissue from 23 pre-menopausal patients was cryopreserved with either slow-freezing or vitrification. After thawing/warming, the tissue was histologically and immunohistochemically analyzed and cultured in vitro. During tissue culture the estradiol release was assessed.

Results

No significant difference was found in the proportion of high-quality follicles after thawing/warming in the slow-freezing and vitrification group, respectively (72.7 versus 66.7 %, p = 0.733). Estradiol secretion by the ovarian tissue was similar between groups during 18 days in vitro culture (area-under-the-curve 5,411 versus 13,102, p = 0.11). Addition of Sphingosine-1-Phosphate or Activin A to the culture medium did not alter estradiol release in both groups. The proportion of Activated Caspase-3 or ‘Proliferating-Cell-Nuclear-Antigen’ positive follicles at the end of the culture period was similar between slow-freezing and vitrification.

Conclusion(s)

Slow-freezing and vitrification result in similar morphological integrity after cryopreservation, a similar estradiol release in culture, and similar rates of follicular proliferation and apoptosis after culture.

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Titel: Slow-freezing versus vitrification for human ovarian tissue cryopreservation
verfasst von: Silke Klocke
Nana Bündgen
Frank Köster
Ursula Eichenlaub-Ritter
Georg Griesinger
Publikationsdatum: 01.02.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 2/2015
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-014-3390-6

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Slow-freezing versus vitrification for human ovarian tissue cryopreservation