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International Journal of Legal Medicine

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01.05.2006 | Technical Note

MiniX-STR multiplex system population study in Japan and application to degraded DNA analysis

verfasst von: H. Asamura, H. Sakai, K. Kobayashi, M. Ota, H. Fukushima

Erschienen in: International Journal of Legal Medicine | Ausgabe 3/2006

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Abstract

We sought to evaluate a more effective system for analyzing X-chromosomal short tandem repeats (X-STRs) in highly degraded DNA. To generate smaller amplicon lengths, we designed new polymerase chain reaction (PCR) primers for DXS7423, DXS6789, DXS101, GATA31E08, DXS8378, DXS7133, DXS7424, and GATA165B12 at X-linked short tandem repeat (STR) loci, devising two miniX-multiplex PCR systems. Among 333 Japanese individuals, these X-linked loci were detected in amplification products ranging in length from 76 to 169 bp, and statistical analyses of the eight loci indicated a high usefulness for the Japanese forensic practice. Results of tests on highly degraded DNA indicated the miniX-STR multiplex strategies to be an effective system for analyzing degraded DNA. We conclude that analysis by the current miniX-STR multiplex systems offers high effectiveness for personal identification from degraded DNA samples.

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Titel: MiniX-STR multiplex system population study in Japan and application to degraded DNA analysis
verfasst von: H. Asamura
H. Sakai
K. Kobayashi
M. Ota
H. Fukushima
Publikationsdatum: 01.05.2006
Verlag: Springer-Verlag
Erschienen in: International Journal of Legal Medicine / Ausgabe 3/2006
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-005-0074-6

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

MiniX-STR multiplex system population study in Japan and application to degraded DNA analysis

Abstract

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Abstract

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