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01.03.2012 | Original Article

Lung function early after lung transplantation is correlated with the frequency of regulatory T cells

verfasst von: Tomoyuki Nakagiri, Gregor Warnecke, Murat Avsar, Stefanie Thissen, Bianca Kruse, Christian Kühn, Petra Ziehme, Ann-Kathrin Knöfel, Nodir Madrahimov, Meinoshin Okumura, Yoshiki Sawa, Jens Gottlieb, André R. Simon, Axel Haverich, Martin Strüber

Erschienen in: Surgery Today | Ausgabe 3/2012

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Abstract

Purposes

Outcomes following lung transplantation are limited by bronchiolitis obliterans syndrome (BOS). As the number of circulating regulatory T cells (Treg) is lower in lung recipients with BOS than in stable lung recipients, we hypothesized that Treg is also correlated with lung function in the early post-transplantation period.

Methods

This prospective study included 18 consecutive patients whose lung function parameters were recorded 3 weeks and 3 months after transplantation, between February and July 2007. Peripheral blood mononuclear cells were stained with anti-CD3, -CD4, -CD8, -CD19, -CD25, -CD28, -CD45RA, -CD45RO, -CD69, -CD127, -CTLA4, and -Foxp3 antibodies and FACS assays were performed. In addition, intracellular cytokines were stained for FACS.

Results

Treg-specific markers (Foxp3, CD127^lo, and CTLA4) in the CD25⁺CD4⁺ population were correlated with both forced expiratory volume in 1 s and forced vital capacity. Th1-cytokine secretion was more dominant in CD4⁺CD25⁺ T cells than in CD4⁺CD25⁻ T cells. In contrast, Th2 and Treg cytokine secretion was the dominant response in stable recipients.

Conclusions

The frequency of Treg cells was positively correlated with good lung function in the early period after lung transplantation.

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Titel: Lung function early after lung transplantation is correlated with the frequency of regulatory T cells
verfasst von: Tomoyuki Nakagiri
Gregor Warnecke
Murat Avsar
Stefanie Thissen
Bianca Kruse
Christian Kühn
Petra Ziehme
Ann-Kathrin Knöfel
Nodir Madrahimov
Meinoshin Okumura
Yoshiki Sawa
Jens Gottlieb
André R. Simon
Axel Haverich
Martin Strüber
Publikationsdatum: 01.03.2012
Verlag: Springer-Verlag
Erschienen in: Surgery Today / Ausgabe 3/2012
Print ISSN: 0941-1291
Elektronische ISSN: 1436-2813
DOI: https://doi.org/10.1007/s00595-011-0087-3

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Lung function early after lung transplantation is correlated with the frequency of regulatory T cells