nach oben

Journal of Natural Medicines

Erschienen in:

07.04.2018 | Original Paper

Toosendanin mediates cisplatin sensitization through targeting Annexin A4/ATP7A in non-small cell lung cancer cells

verfasst von: Mao Dong Zheng, Nai Dong Wang, Xiao Liang Li, Juan Yan, Jian Hua Tang, Xiu Hua Zhao, Zhihua Zhang

Erschienen in: Journal of Natural Medicines | Ausgabe 3/2018

Einloggen, um Zugang zu erhalten

Abstract

Cisplatin (CDDP) is used in the treatment of non-small cell lung cancer (NSCLC), but due to the development of resistance, the benefit has been limited. Toosendanin (TSN) has shown therapeutic effects on NSCLC; however, the role of TSN on CDDP sensitization in NSCLC remains unknown. The antitumor effects of TSN and CDDP sensitization mediated by TSN were explored. TSN was added in various amounts to measure dose- and time-dependent cytotoxicity. Intracellular CDDP was detected by high-performance liquid chromatography. The protein levels of ATP7A, ATP7B, hCTR1, MRP-2, P-gp and Annexin A4 (Anxa4) were analyzed. The tests were conducted using normal NSCLC (A549 cell line) and CDDP-resistant cells (A549/DDP cell line). Anxa4 promotes CDDP resistance by regulating ATP7A, so Anxa4 was overexpressed and silenced and also transfected with pcMV6 or siRNA/ATP7A, respectively. Mechanistic investigations revealed that TSN decreased relative viability in NSCLC cells. Remarkably, TSN significantly enhanced CDDPsensitization in invalid doses. TSN downregulated Anxa4 expression, enhanced intracellular CDDP, and had no effect on MRP-2, P-gp, ATP7A, ATP7B or hCTR1. Subsequently, overexpression of Anxa4 led to a significant decrease in intracellular CDDP concentration. The adjustment of CDDP concentration regulated by TSN disappeared in Anxa4 or ATP7A-silenced cells. TSN also enhanced CDDP sensitization in single ATP7A-overexpressing cells, but had no effect on cells with simultaneous ATP7A overexpression and Anxa4 silencing. The present study suggests that TSN can mediate CDDP sensitization in NSCLC through downregulation of Anxa4.

Hildebrandt MA, Gu J, Wu X (2009) Pharmacogenomics of platinum-based chemotherapy in NSCLC. Expert Opin Drug Metab Toxicol 5(7):745–755CrossRef

Siegel RL, Miller KD, Jemal A (2017) Cancer statistics, 2017. CA Cancer J Clin 67(1):7–30CrossRef

Zornosa C, Vandergrift JL, Kalemkerian GP, Ettinger DS, Rabin MS, Reid M, Otterson GA, Koczywas M, D’Amico TA, Niland JC, Mamet R, Pisters KM (2012) First-line systemic therapy practice patterns and concordance with NCCN guidelines for patients diagnosed with metastatic NSCLC treated at NCCN institutions. J Natl Compr Canc Netw 10(7):847–856CrossRef

Perez RP (1998) Cellular and molecular determinants of cisplatin resistance. Eur J Cancer 34(10):1535–1542CrossRef

Tiseo M, Franciosi V, Grossi F, Ardizzoni A (2006) Adjuvant chemotherapy for non-small cell lung cancer: ready for clinical practice? Eur J Cancer 42(1):8–16CrossRef

Martin LP, Hamilton TC, Schilder RJ (2008) Platinum resistance: the role of DNA repair pathways. Clin Cancer Res 14(5):1291–1295CrossRef

Stewart DJ (2007) Mechanisms of resistance to cisplatin and carboplatin. Crit Rev Oncol Hematol 63(1):12–31CrossRef

Zisowsky J, Koegel S, Leyers S, Devarakonda K, Kassack MU, Osmak M, Jaehde U (2007) Relevance of drug uptake and efflux for cisplatin sensitivity of tumor cells. Biochem Pharmacol 73(2):298–307CrossRef

Ling CQ, Yue XQ, Ling C (2014) Three advantages of using traditional Chinese medicine to prevent and treat tumor. J Integr Med 12(4):331–335CrossRef

10.

Tang MZ, Wang ZF, Shi YL (2004) A study of ultrasound-assisted extraction of toosendanin with water from bark of melia toosendan sieb. T zucc. J Southwest Univ Natl (Nat Sci Ed, Chin) 30(8):714–717

11.

Tang MZ, Wang ZF, Shi YL (2004) Involvement of cytochrome c release and caspase activation in toosendanin-induced PC12 cell apoptosis. Toxicology 201(1–3):131–138

12.

Yu JC, Min ZD, Ip NY (2004) Melia toosendan regulates PC12 Cell differentiation via the activation of protein kinase A and extracellular signal-regulated kinases. Neurosignals 13(5):248–257CrossRef

13.

Zhang B, Wang ZF, Tang MZ, Shi YL (2005) Growth inhibition and apoptosis-induced effect on human cancer cells of toosendanin, a triterpenoid derivative from chinese traditional medicine. Invest New Drugs 23(6):547–553CrossRef

14.

Wang G, Feng CC, Chu SJ, Zhang R, Lu YM, Zhu JS, Zhang J (2015) Toosendanin inhibits growth and induces apoptosis in colorectal cancer cells through suppression of AKT/GSK-3β/β-catenin pathway. Int J Oncol 47(5):1767–1774CrossRef

15.

Harrington CF, Le Pla RC, Jones GD, Thomas AL, Farmer PB (2010) Determination of cisplatin 1,2-intrastrand guanine-guanine DNA adducts in human leukocytes by high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry. Chem Res Toxicol 23(8):1313–1321CrossRef

16.

Cui Y, König J, Buchholz JK, Spring H, Leier I, Keppler D (1999) Drug resistance and ATP-dependent conjugate transport mediated by the apical multidrug resistance protein, MRP2, permanently expressed in human and canine cells. Mol Pharmacol 55(5):929–937PubMed

17.

Samimi G, Varki NM, Wilczynski S, Safaei R, Alberts DS, Howell SB (2003) Increase in expression of the copper transporter ATP7A during platinum drug-based treatment is associated with poor survival in ovarian cancer patients. Clin Cancer Res 9(16 Pt 1):5853–5859PubMed

18.

Komatsu M, Sumizawa T, Mutoh M, Chen ZS, Terada K, Furukawa T, Yang XL, Gao H, Miura N, Sugiyama T, Akiyama S (2000) Copper-transporting P-type adenosine triphosphatase (ATP7B) is associated with cisplatin resistance. Cancer Res 60(5):1312–1316PubMed

19.

Ishida S, Lee J, Thiele DJ, Herskowitz I (2002) Uptake of the anticancer drug cisplatin mediated by the copper transporter Ctr1 in yeast and mammals. Proc Natl Acad Sci USA 99(22):14298–14302CrossRef

20.

Li X, Huang JM, Wang JN, Xiong XK, Yang XF, Zou F (2015) Combination of chrysin and cisplatin promotes the apoptosis of HepG2 cells by up-regulating p53. Chem Biol Interact 232:212–220

21.

Inoue Y, Matsumoto H, Yamada S, Kawai K, Suemizu H, Gika M, Takanami I, Iwazaki M, Nakamura M (2010) Association of ATP7A expression and in vitro sensitivity to cisplatin in non-small cell lung cancer. Oncol Lett 1(5):837–840CrossRef

22.

Li ZH, Qiu MZ, Zeng ZL, Luo HY, Wu WJ, Wang F, Wang ZQ, Zhang DS, Li YH, Xu RH (2012) Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum resistance in non-small cell lung cancer (NSCLC). J Transl Med 10:21CrossRef

23.

Yang T, Chen M, Chen T, Thakur A (2015) Expression of the copper transporters hCtr1, ATP7A and ATP7B is associated with the response to chemotherapy and survival time in patients with resected non-small cell lung cancer. Oncol Lett 10(4):2584–2590CrossRef

24.

Gerke V, Creutz CE, Moss SE (2005) Annexins: linking Ca²⁺ signalling to membrane dynamics. Nat Rev Mol Cell Biol 6(6):449–461CrossRef

25.

Han EK, Tahir SK, Cherian SP, Collins N, Ng SC (2000) Modulation of paclitaxel resistance by annexin IV in human cancer cell lines. Br J Cancer 83(1):83–88CrossRef

26.

Wei R, Zhang Y, Shen L, Jiang W, Li C, Zhong M, Xie Y, Yang D, He L, Zhou Q (2012) Comparative proteomic and radiobiological analyses in human lung adenocarcinoma cells. Mol Cell Biochem 359(1–2):151–159CrossRef

27.

Kim A, Enomoto T, Serada S, Ueda Y, Takahashi T, Ripley B, Miyatake T, Fujita M, Lee CM, Morimoto K, Fujimoto M, Kimura T, Naka T (2009) Enhanced expression of Annexin A4 in clear cell carcinoma of the ovary and its association with chemoresistance to carboplatin. Int J Cancer 125(10):2316–2322CrossRef

28.

Matsuzaki S, Enomoto T, Serada S, Yoshino K, Nagamori S, Morimoto A, Yokoyama T, Kim A, Kimura T, Ueda Y, Fujita M, Fujimoto M, Kanai Y, Kimura T, Naka T (2014) Annexin A4-conferred platinum resistance is mediated by the copper transporter ATP7A. Int J Cancer 134(8):1796–1809CrossRef

Titel: Toosendanin mediates cisplatin sensitization through targeting Annexin A4/ATP7A in non-small cell lung cancer cells
verfasst von: Mao Dong Zheng
Nai Dong Wang
Xiao Liang Li
Juan Yan
Jian Hua Tang
Xiu Hua Zhao
Zhihua Zhang
Publikationsdatum: 07.04.2018
Verlag: Springer Singapore
Erschienen in: Journal of Natural Medicines / Ausgabe 3/2018
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-018-1211-0

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Toosendanin mediates cisplatin sensitization through targeting Annexin A4/ATP7A in non-small cell lung cancer cells

Abstract

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2018

Four new resin glycosides, murasakimasarins I–IV, from the tuber of Ipomoea batatas

Mitogenic activity of Artocarpus lingnanensis lectin and its apoptosis induction in Jurkat T cells

Anti-amyloidgenic and neurotrophic effects of tetrahydroxystilbene glucoside on a chronic mitochondrial dysfunction rat model induced by sodium azide

Determination of (E)-ferulic acid content in the root of Angelica acutiloba: a simple chemical evaluation method for crude drug quality control

Anti-inflammatory and anti-angiogenic activities in vitro of eight diterpenes from Daphne genkwa based on hierarchical cluster and principal component analysis

A bibenzyl from Dendrobium ellipsophyllum induces apoptosis in human lung cancer cells