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Erschienen in:
01.08.2011 | Original Research Paper
Soluble vascular endothelial growth factor (VEGF) receptor-1 inhibits migration of human monocytic THP-1 cells in response to VEGF
Erschienen in: Inflammation Research | Ausgabe 8/2011
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Objective
We aimed to investigate the regulation and contribution of vascular endothelial growth factor (VEGF) and sFlt-1(1–3) to human monocytic THP-1 migration.
Materials and methods
Ad-sFlt-1/FLAG, a recombinant adenovirus carrying the human sFlt-1(1–3) (the first three extracellular domains of FLT-1, the hVEGF receptor-1) gene, was constructed. L929 cells were infected with Ad-sFlt-1/FLAG and the expression of sFlt-1 was detected by immunofluorescent assay and ELISA. Corning® Transwell® Filter Inserts containing polyethylene terephthalate (PET) membranes with pore sizes of 3 μm were used as an experimental model to simulate THP-1 migration. Five VEGF concentrations (0, 0.1, 1, 10 and 100 ng/ml), four concentrations of sFlt-1(1–3)/FLAG expression supernatants (0.1, 1, 10 and 100 ng/ml), and monocyte chemoattractant protein-1 (MCP-1, 10 ng/ml) were used to test the ability of THP-1 cells to migrate through PET membranes.
Results
The sFlt-1(1–3) gene was successfully recombined into Ad-sFlt-1/FLAG. sFlt-1(1–3) was expressed in L929 cells transfected with Ad-sFlt-1/FLAG. THP-1 cell migration increased with increasing concentrations of VEGF, while cell migration decreased with increasing concentrations of sFlt1(1–3)/FLAG. sFlt1(1–3)/FLAG had no effect on MCP-1-induced cell migration.
Conclusions
This study demonstrated that VEGF is able to elicit a migratory response in THP-1 cells, and that sFlt-1(1–3) is an effective inhibitor of THP-1 migration towards VEGF.