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European Radiology

Erschienen in:

01.02.2015 | Experimental

Assessment of diabetic peripheral neuropathy in streptozotocin-induced diabetic rats with magnetic resonance imaging

verfasst von: Dongye Wang, Xiang Zhang, Liejing Lu, Haojiang Li, Fang Zhang, Yueyao Chen, Jun Shen

Erschienen in: European Radiology | Ausgabe 2/2015

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Abstract

Objective

To determine the role of magnetic resonance (MR) imaging and quantitative T2 value measurements in the assessment of diabetic peripheral neuropathy (DPN).

Methods

Sequential MR imaging, T2 measurement, and quantitative sensory testing of sciatic nerves were performed in streptozotocin-induced diabetic rats (n = 6) and normal control rats (n = 6) over a 7-week follow-up period. Histological assessment was obtained from 48 diabetic rats and 48 control rats once weekly for 7 weeks (n = 6 for each group at each time point). Nerve signal abnormalities were observed, and the T2 values, mechanical withdrawal threshold (MWT), and histological changes were measured and compared between diabetic and control animals.

Results

Sciatic nerves in the diabetic rats showed a gradual increase in T2 values beginning at 2 weeks after the induction (P = 0.014), while a decrease in MWT started at 3 weeks after the induction (P = 0.001). Nerve T2 values had a similar time course to sensory functional deficit in diabetic rats. Histologically, sciatic nerves of diabetic rats demonstrated obvious endoneural oedema from 2 to 3 weeks after the induction, followed by progressive axonal degeneration, Schwann cell proliferation, and coexistent disarranged nerve regeneration.

Conclusion

Nerve T2 measurement is potentially useful in detecting and monitoring diabetic neuropathy.

Key points

• Sciatic nerves in diabetic rats showed a gradual increase in T2 values

• Nerve T2 values were negatively correlated with sensory function impairment

• Longitudinal T2 values can be used to monitor the disease progress

• Nerve degeneration contributed mainly to progressive prolongation of nerve T2 values

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Titel: Assessment of diabetic peripheral neuropathy in streptozotocin-induced diabetic rats with magnetic resonance imaging
verfasst von: Dongye Wang
Xiang Zhang
Liejing Lu
Haojiang Li
Fang Zhang
Yueyao Chen
Jun Shen
Publikationsdatum: 01.02.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: European Radiology / Ausgabe 2/2015
Print ISSN: 0938-7994
Elektronische ISSN: 1432-1084
DOI: https://doi.org/10.1007/s00330-014-3423-3

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Springer Medizin

Assessment of diabetic peripheral neuropathy in streptozotocin-induced diabetic rats with magnetic resonance imaging

Abstract

Objective

Methods

Results

Conclusion

Key points

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Springer Medizin

Abstract

Objective

Methods

Results

Conclusion

Key points

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