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Journal of Cancer Research and Clinical Oncology

Erschienen in:

01.02.2016 | Original Article – Cancer Research

Cancer cell invasion driven by extracellular matrix remodeling is dependent on the properties of cancer-associated fibroblasts

verfasst von: Shinya Neri, Hiroko Hashimoto, Hiroaki Kii, Hirotada Watanabe, Kenkichi Masutomi, Takeshi Kuwata, Hiroshi Date, Masahiro Tsuboi, Koichi Goto, Atsushi Ochiai, Genichiro Ishii

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 2/2016

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Abstract

Purpose

As one form of tumor invasion, cancer cells can invade the extracellular matrix (ECM) through tracks that have been physically remodeled by cancer-associated fibroblasts (CAFs). However, CAFs are a heterogeneous population with diverse matrix-remodeling capacities. The purpose of this study was to investigate how CAFs with various matrix-remodeling capacities influence cancer cell invasion.

Methods

We established single-cell-derived clones from three primary cultures of CAFs from lung adenocarcinoma patients (Case 1, 5 clones; Case 2, 5 clones; and Case 3, 7 clones). Using a co-culture model, we evaluated the correlations between the number of invaded cancer cells and the remodeling areas generated by CAF clones in each case.

Results

When A549 lung adenocarcinoma cells and CAF clones were co-cultured, both the numbers of invaded cancer cells and the remodeling areas generated by the CAF clones varied greatly. The number of invaded cancer cells was moderately and strongly correlated with the remodeling areas generated by each CAF clone originating from Cases 1 and 2 (R ² value = 0.53 and 0.68, respectively), suggesting that the remodeling areas in the ECM may determine the number of invaded cancer cells. In contrast, the number of invaded cancer cells was not correlated with the remodeling areas generated by CAF clones originating from Case 3, suggesting that factors other than the remodeling areas might determine the number of invading cancer cells.

Conclusions

These findings showed two types of fibroblast-dependent cancer cell invasion that are dependent on and independent of the remodeling areas generated by CAFs.

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Titel: Cancer cell invasion driven by extracellular matrix remodeling is dependent on the properties of cancer-associated fibroblasts
verfasst von: Shinya Neri
Hiroko Hashimoto
Hiroaki Kii
Hirotada Watanabe
Kenkichi Masutomi
Takeshi Kuwata
Hiroshi Date
Masahiro Tsuboi
Koichi Goto
Atsushi Ochiai
Genichiro Ishii
Publikationsdatum: 01.02.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 2/2016
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-015-2046-7

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Cancer cell invasion driven by extracellular matrix remodeling is dependent on the properties of cancer-associated fibroblasts