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01.06.2014 | Original Article

The impact of pretreatment with bolus dose of enteral glutamine on acute lung injury induced by oleic acid in rats

verfasst von: A. Ebru Salman, Fahri Yetişir, Mehmet Kılıç, Özkan Önal, Ahmet Dostbil, Dilara Zeybek, Mustafa Aksoy, Figen Kaymak, Tuğrul Çelik, Süheyla Ünver

Erschienen in: Journal of Anesthesia | Ausgabe 3/2014

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Abstract

Purpose

Both parenteral and enteral glutamine have shown beneficial effects in sepsis and ischemia/reperfusion-induced acute lung injury (ALI). Oleic acid (OA) has been used to induce ALI in experimental studies. In this study, we investigated the effects of pretreatment of a bolus dose of enteral glutamine on ALI induced by OA in rats.

Methods

Twenty-eight adult female Sprague–Dawley rats weighing 240–300 g were divided into four groups, 7 in each. Group I and group II received normal saline for 30 days, group III and group IV received glutamine at a dose of 1 g/kg for 10 days by gavage, and in group II and group IV 100 mg/kg OA was administered i.v. Histopathological examination of the lung was performed with light and electron microscopy. Levels of protein carbonyl, malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase levels were measured in tissue samples. Levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10, and total tissue oxidant status and total tissue antioxidant status were measured in serum samples.

Results

Light microscopy showed that the total lung injury score of group IV was significantly lower than group II. Change in thickness of the fused basal lamina was not significantly different in groups II and IV under electron microscopy. TNF-α, IL-6, and IL-10 serum levels were higher in group II when compared to group I and significantly attenuated in group IV.

Conclusion

Pretreatment with a bolus dose of enteral glutamine minimized the extent of ALI induced by OA in rats.

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Titel: The impact of pretreatment with bolus dose of enteral glutamine on acute lung injury induced by oleic acid in rats
verfasst von: A. Ebru Salman
Fahri Yetişir
Mehmet Kılıç
Özkan Önal
Ahmet Dostbil
Dilara Zeybek
Mustafa Aksoy
Figen Kaymak
Tuğrul Çelik
Süheyla Ünver
Publikationsdatum: 01.06.2014
Verlag: Springer Japan
Erschienen in: Journal of Anesthesia / Ausgabe 3/2014
Print ISSN: 0913-8668
Elektronische ISSN: 1438-8359
DOI: https://doi.org/10.1007/s00540-013-1745-y

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The impact of pretreatment with bolus dose of enteral glutamine on acute lung injury induced by oleic acid in rats