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01.12.2011 | Original Article

High genetic variability and recombination in a begomovirus population infecting the ubiquitous weed Cleome affinis in northeastern Brazil

verfasst von: Sarah J. C. da Silva, Gloria P. Castillo-Urquiza, Braz T. Hora Júnior, Iraildes P. Assunção, Gaus S. A. Lima, Gilvan Pio-Ribeiro, Eduardo S. G. Mizubuti, F. Murilo Zerbini

Erschienen in: Archives of Virology | Ausgabe 12/2011

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Abstract

Diseases caused by begomoviruses are a serious constraint to crop production in many tropical and subtropical areas of the world, including Brazil. Begomoviruses are whitefly-transmitted, single-stranded DNA viruses that are often associated with weed plants, which may act as natural reservoirs of viruses that cause epidemics in crop plants. Cleome affinis (family Capparaceae) is an annual weed that is frequently associated with leguminous crops in Brazil. Samples of C. affinis were collected in four states in the northeast of Brazil. Analysis of 14 full-length DNA-A components revealed that only one begomovirus was present, with 91-96% identity to cleome leaf crumple virus (ClLCrV). In a phylogenetic tree, ClLCrV forms a basal group relative to all other Brazilian begomoviruses. Evidence of multiple recombination events was detected among the ClLCrV isolates, which also display a high degree of genetic variability. Despite ClLCrV being the only begomovirus found, its phylogenetic placement, high genetic variability and recombinant nature suggest that C. affinis may act as a source of novel viruses for crop plants. Alternatively, ClLCrV could be a genetically isolated begomovirus. Further studies on the biological properties of ClLCrV should help to clarify the role of C. affinis in the epidemiological scenario of Brazilian begomoviruses.

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Titel: High genetic variability and recombination in a begomovirus population infecting the ubiquitous weed Cleome affinis in northeastern Brazil
verfasst von: Sarah J. C. da Silva
Gloria P. Castillo-Urquiza
Braz T. Hora Júnior
Iraildes P. Assunção
Gaus S. A. Lima
Gilvan Pio-Ribeiro
Eduardo S. G. Mizubuti
F. Murilo Zerbini
Publikationsdatum: 01.12.2011
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 12/2011
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-011-1119-4

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