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01.10.2014 | Original Paper

BMP9 regulates cross-talk between breast cancer cells and bone marrow-derived mesenchymal stem cells

verfasst von: Shaoheng Wan, Yuehong Liu, Yaguang Weng, Wei Wang, Wei Ren, Chang Fei, Yingying Chen, Zhihui Zhang, Ting Wang, Jinshu Wang, Yayun Jiang, Lan Zhou, Tongchuan He, Yan Zhang

Erschienen in: Cellular Oncology | Ausgabe 5/2014

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Abstract

Purpose

Breast cancer cells frequently metastasize to distant organs, including bone. Interactions between breast cancer cells and the bone microenvironment are known to enhance tumor growth and osteolytic damage. Here we investigated whether BMP9 (a secretary protein) may change the bone microenvironment and, by doing so, regulate the cross-talk between breast cancer cells and bone marrow-derived mesenchymal stem cells.

Methods

After establishing a co-culture system composed of MDA-MB-231breast cancer cells and HS-5 bone marrow-derived mesenchymal stem cells, and exposure of this system to BMP9 conditioned media, we assessed putative changes in migration and invasion capacities of MDA-MB-231 cells and concomitant changes in osteogenic marker expressionin HS-5 cells and metastases-related genes in MDA-MB-231 cells.

Results

We found that BMP9 can inhibit the migration and invasion of MDA-MB-231 cells, and promote osteogenesis and proliferation of HS-5 cells, in the co-culture system. We also found that the BMP9-induced inhibition of migration and invasion of MDA-MB-231 cells may be caused by a decreased RANK ligand (RANKL) secretion by HS-5 cells, leading to a block in the AKT signaling pathway.

Conclusions

From our data we conclude that BMP9 inhibits the migration and invasion of breast cancer cells, and promotes the osteoblastic differentiation and proliferation of bone marrow-derived mesenchymal stem cells by regulating cross-talk between these two types of cells through the RANK/RANKL signaling axis.

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Titel: BMP9 regulates cross-talk between breast cancer cells and bone marrow-derived mesenchymal stem cells
verfasst von: Shaoheng Wan
Yuehong Liu
Yaguang Weng
Wei Wang
Wei Ren
Chang Fei
Yingying Chen
Zhihui Zhang
Ting Wang
Jinshu Wang
Yayun Jiang
Lan Zhou
Tongchuan He
Yan Zhang
Publikationsdatum: 01.10.2014
Verlag: Springer Netherlands
Erschienen in: Cellular Oncology / Ausgabe 5/2014
Print ISSN: 2211-3428
Elektronische ISSN: 2211-3436
DOI: https://doi.org/10.1007/s13402-014-0197-1

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