Background
Methods
Study subjects
Infection procedures
Sample collection and processing
Real-time PCR assay
Data analysis
Results
Time to parasitaemia
Mean | Std Dev | Max | Min | Median | 25% | 75% | P Value | |
---|---|---|---|---|---|---|---|---|
First study (n = 11)
| ||||||||
Smear
| 11.34 | 0.93 | 13.02 | 10.11 | 10.96 | 10.91 | 11.97 | 0.0001 |
qPCR
| 7.01 | 0.07 | 7.12 | 6.91 | 6.98 | 6.95 | 7.07 | |
Second study (n = 5)
| ||||||||
Smear
| 12.54 | 1.33 | 14.00 | 10.96 | 11.98 | 11.43 | 13.94 | 0.0004 |
qPCR
| 6.38 | 0.89 | 7.01 | 5.01 | 6.93 | 5.47 | 7.00 |
Parasite density
Parasite multiplication rate and growth
1st cycle (95% CI) | 2nd cycle (95% CI) | 3rd cycle (95% CI) | 4th cycle (95% CI) | |
---|---|---|---|---|
First study
| 0.44 (0.27 to 0.72) | 2.41 (1.33 to 4.37) | 24.55 (16.87 to 35.73) | Nil |
Second study
| 0.20 (0.11 to 0.38) | 0.29 (0.14 to 0.64) | 3.71 (0.41 to 33.86) | 14.15 (3.36 to 59.66) |
Effect of parasite sequestration in qPCR detection
ID | Date | Cq | CqMean | CqSD | ID | Date | Cq | CqMean | CqSD |
---|---|---|---|---|---|---|---|---|---|
01 02FEB
|
42.08
|
42.08
|
01 03FEB
|
30.58
|
31.02
|
0.49
| |||
Und
|
42.08
|
31.54
|
31.02
|
0.49
| |||||
Und
|
42.08
|
30.92
|
31.02
|
0.49
| |||||
Und | 33.76 | 0.98 | 31.05 | 30.86 | 0.32 | ||||
32.97 | 33.76 | 0.98 | 30.51 | 30.86 | 0.32 | ||||
34.92 | 33.76 | 0.98 | 31.24 | 30.86 | 0.32 | ||||
Und | 33.76 | 0.98 | 30.95 | 30.86 | 0.32 | ||||
34.74 | 33.76 | 0.98 | 30.30 | 30.86 | 0.32 | ||||
33.00 | 33.76 | 0.98 | 31.18 | 30.86 | 0.32 | ||||
33.13 | 33.76 | 0.98 | 30.82 | 30.86 | 0.32 | ||||
Und | 33.76 | 0.98 | 30.82 | 30.86 | 0.32 | ||||
ID
|
Date
|
C
q
|
C
q
Mean
|
C
q
SD
|
ID
|
Date
|
C
q
|
C
q
Mean
|
C
q
SD
|
02 02FEB
|
34.47
|
34.47
|
02 03FEB
|
Und
| |||||
Und
|
34.47
|
Und
| |||||||
Und
|
34.47
|
Und
| |||||||
37.30 | 36.22 | 2.01 | 33.93 | 34.34 | 0.68 | ||||
Und | 36.22 | 2.01 | 34.99 | 34.34 | 0.68 | ||||
33.28 | 36.22 | 2.01 | Und | 34.34 | 0.68 | ||||
37.30 | 36.22 | 2.01 | Und | 34.34 | 0.68 | ||||
35.05 | 36.22 | 2.01 | Und | 34.34 | 0.68 | ||||
Und | 36.22 | 2.01 | 34.84 | 34.34 | 0.68 | ||||
Und | 36.22 | 2.01 | 33.60 | 34.34 | 0.68 | ||||
38.17 | 36.22 | 2.01 | Und | 34.34 | 0.68 |