Introduction
Skeletal muscle is the most abundant tissue in the human body, aside from its primary role in locomotion it is also the primary site of oxidative metabolism, insulin-stimulated glucose uptake [
1] and amino acid uptake [
2]. Muscle size is regulated by the balance between muscle protein synthesis (MPS) and muscle protein breakdown (MPB) [
3]. In the fasted state MPS is suppressed and MPB is elevated such that the muscle is in a net catabolic state [
4]. In young healthy individuals the ingestion of a protein containing meal results in an elevation in MPS and a suppression of MPB resulting in a net anabolic state [
4]. In healthy young individuals postabsorptive catabolism and postprandial anabolism are equal in the long term and muscle size is maintained [
3]. MPS appears to be much more tightly regulated than MPB [
5] and is primarily regulated though the mammalian target of the rapamycin (mTOR) pathway which is sensitive to the effects of growth factors such as insulin, nutrients and contraction [
6]. The branched chain amino acid (BCAA) leucine has been shown to be the primary nutrient regulator of the mTOR pathway [
7].
The consumption of intact protein or an amino acid mixture containing leucine results in a robust phosphorylation of p70S6 kinase (p70S6K), a downstream target of the mTOR pathway [
8‐
10]. p70S6K phosphorylation leads to increases in the initiation of protein translation and ultimately MPS. Short term physical inactivity appears to lead to a decreased MPS response to protein feeding; however, changes in the response of the mTOR pathway are less clear [
11‐
13]. In many but not all studies, old age is associated with an ‘anabolic resistance’ to protein feeding where MPS is not elevated in response to feeding in the elderly. This anabolic resistance appears to be mediated through decreased sensitivity of the mTOR pathway to feeding [
9,
14]. It is unclear if ageing
per se is the cause of impaired anabolic signalling or if a decline in physical activity and a different metabolic phenotype are the underlying causes of the observed anabolic signalling deficits.
Many studies have examined the effects of free amino acid or intact protein ingestion on MPS and mTOR signaling [
9,
15].
These studies have shown that proteins high in the BCAA leucine result in greater mTOR activation compared with proteins containing less leucine [
8,
15‐
17]. Dairy and soy food are sources of dietary protein; because of its greater leucine content, milk protein appears to results in a greater MPS response than soy protein, at least after resistance exercise [
18]. There have been a few recent studies which have examined MPS and mTOR pathway response to the ingestion of mixed macronutrient beverages; however, there is very little research on the ingestion of true mixed meal on activation of the mTOR pathway [
15,
19]. There is evidence that the addition of fat, carbohydrate and fiber to protein may slow the appearance of amino acids in the blood and thus blunt the anabolic signalling response [
20‐
22]. There is also evidence that consuming solid food rather than a liquid supplement results in a slower rate of appearance of amino acids in the blood [
22].
Metabolic syndrome (MetS) is a characterized by a cluster of conditions which include central obesity, dyslipidemia, high blood pressure and insulin resistance [
23]. There has been little research on MetS and protein metabolism; however, older type 2 diabetics display lower muscle strength, mass, and quality compared to age matched controls [
24]. Type 2 diabetics also display an insulin resistance of protein metabolism at the whole body level [
25]. However, they are able to normally activate some members of the mTOR pathway in muscle during a hyperinsulinaemic hyperaminoacidaemic clamp [
26]. Little is known about the muscle anabolic response of those with MetS under more physiological conditions such as mixed meal consumption.
Therefore the objectives of the current study were three fold. Firstly, to determine whether ingestion of a single breakfast meal results in the activation of the mTOR signalling pathway in middle aged men. Secondly, to examine whether meals differing in amino acid composition, yet matched for total energy and macronutrient composition, result in altered mTOR signalling. Lastly, to investigate if middle aged men with MetS display a resistance of anabolic signalling to mixed meal ingestion compared with healthy controls.
Methods
Subjects
A total of 20 men (
n = 10 healthy controls,
n = 10 MetS) aged between 40–60 years were recruited from newspaper, poster, and flyer advertisements to participate. Subjects were classified as having MetS based on the International Diabetes Federation criteria [
27]. They had to present with abdominal obesity (waist circumference ≥ 94 cm) and two of the following factors: raised serum triglycerides (≥1.7 mmol/l), reduced serum HDL cholesterol (<1.03 mmol/l), impaired fasting glycaemia (fasting plasma glucose ≥5.6 mmol/l) or raised blood pressure (systolic blood pressure ≥130 mmHg or diastolic blood pressure ≥85 mmHg). A cohort of age and height matched healthy controls, without MetS were also included. Subjects were excluded if they showed evidence of acute or chronic inflammatory disease, infectious diseases, cancer, and/or known alcohol consumption (>20 g per day). Subjects with fasting glucose concentrations indicative of T2DM were also excluded along with people on diabetic medications. All experimental procedures were performed in accordance with the Helsinki declaration of 1975 as revised in 1983 and were formally approved by the Deakin University Human Research Ethics Committee (EC-120, 2008). Informed written consent was obtained from each subject before participation in the study and after the nature, purpose, and risks of the study were explained and subjects informed of their right to withdraw from the trial at any stage of the investigation.
Experimental design
A controlled crossover single meal study was conducted to examine the postprandial effects to either a dairy or soy meal, with at least four weeks wash out between the study days. Subjects were instructed to abstain from alcohol, caffeine and tobacco on the day proceeding the trial day. On the morning of the trial, subjects presented to the Deakin University clinical laboratory in a fasted state. Upon arrival, they had their height, weight and blood pressure measured. A cannula was inserted in the antecubital vein and a fasting blood sample was collected. Blood samples were collected 30 min, 60 min, 180 min and 240 min following the test meal for plasma amino acid analysis. Following 30 min of supine resting, a muscle sample was collected from the
vastus lateralis under local anesthesia (Xylocaine 1%) by percutaneous needle biopsy technique [
28] modified to include suction [
29]. Muscle tissue from each biopsy was immediately frozen and stored in liquid nitrogen for later analysis. Following this, subjects were required to consume a high fat dairy or high fat soy meal within approximately 15 min. The details of the study design and meal have been previously reported [
30]. Further muscle samples were collected at 2 h and 4 h post meal ingestion with serial biopsy samples collected at least 2 cm from previous biopsy sites.
Test meals
Subjects were randomly assigned to consume either a breakfast meal comprised of dairy-derived protein or void of dairy-derived proteins (replaced with the same level of soy based protein). The interval between the two test meals was at least four weeks. To prevent possible differences between subjects at baseline from their previous meal the night prior to the study day, subjects were provided with a controlled meal for dinner. The meal consisted of a pre-packaged lasagna and fruit yogurt providing a total of 2462 kJ as 20% fat, 18% protein and 62% carbohydrates. Subjects were asked to eat only the provided food and nothing else. The test breakfast meals consisted of cheese, butter, and full cream milk with white bread toast (dairy breakfast) and the second meal contained soy cheese analogue, soy beverage, a soy spread, and white bread toast and contained the same amount of protein (31 g) with similar carbohydrate content (Table
1). As the test meals contained different sources of protein, the amino acid composition also differed (refer to Table
2). Subjects were asked to consume the entire breakfast meal within 15 min.
Table 1
Energy content and macronutrient composition of the high fat meals
Soy
| Margarine | 20 g | 3276 | 31 | 54 | 48 | 1297 |
Full fat soy milk | 300 mL |
Soy cheese | 100 g |
Bread (white) | 50 g |
Dairy
| Butter | 23 g | 3120 | 31 | 54 | 37 | 951 |
Full cream milk | 300 mL |
Cheese | 70 g |
Bread (white) | 50 g |
Table 2
Amino acid composition of the test meals
| | mg/g (%) | |
Histidine | 2.1 | | 2.3 |
Serine | 6.7 | | 6.8 |
Arginine | 5.2 | | 2.8 |
Glycine | 7.2 | | 3.8 |
Aspartic acid | 9.5 | | 6.4 |
Glutamic acid | 21.5 | | 21.6 |
Threonine | 4.0 | | 4.1 |
Alanine | 5.9 | | 4.5 |
Proline | 8.1 | | 11.8 |
Lysine | 4.8 | | 6.2 |
Tyrosine | 2.1 | | 3.0 |
Methionine | 1.1 | | 2.2 |
Valine
|
5.3
| |
6.6
|
Isoleucine
|
4.5
| |
4.7
|
Leucine
|
7.8
| |
9.1
|
Phenylalanine | 4.3 | | 4.0 |
Total BCAA
|
17.6
| |
20.4
|
Quantitative amino acid analyses of test meals
Test meals were homogenized and underwent liquid hydrolysis in 6 M HCl at 110°C for 24 h. Following hydrolysis, amino acid derivation was conducted using AccQ-Tag reagents as per manufacturer’s instructions. Liquid chromatographic analysis was performed on a Waters Acquity UPLC system, equipped with a binary solvent manager, an autosampler, a column heater, a PDA detector, and interfaced to a tandem quadrupole detector at the Australian Proteome Analysis Facility (APAF; Macquarie University, New South Wales, Australia) [
31].
Anthropometric measurements
Weight, height and waist circumference were measured at baseline and upon arrival to the clinical laboratory following an overnight fast on test days. Blood pressure was measured on three occasions using a mercury sphygmomanometer.
Biochemical measurements
Venous blood samples were drawn at fasting, 30 min, 60 min, 180 min, and 240 min after consumption of the meal using EDTA tubes. EDTA blood samples were centrifuged at 3000
rpm for 15 min and 300 μL of separated plasma removed for subsequent amino acid analysis. Blood samples were also collected in serum tubes, centrifuged at 3000
rpm for 15 min, stood for 30 min at room temperature, and were then supplied to Cabrini Pathology (Cabrini Health, Victoria, Australia) for assessment of insulin and glucose. To estimate insulin resistance, HOMA index was calculated by the formula: HOMA = (fasting plasma insulin in μU/mL × fasting plasma glucose in mM)/22.5 [
32,
33].
Plasma amino acids
Quantification of plasma amino acids was carried out by reverse-phase high-performance liquid chromatography (HPLC) with pre-column derivitisation of plasma samples with purified 6-aminoquinolyl-
N-hydroxysuccinimidyl carbamate (AQC; School of Botany, University of Melbourne, Victoria, Australia) followed by reverse-phase HPLC [
31]. Briefly, 50 μL of plasma was incubated with 100 μL of chilled acetonitrile (ACN) to precipitate plasma proteins. Samples were briefly vortexed and centrifuged for 10 min at 0°C before 10 μL of the resulting supernatant was aliquoted into an HPLC vial with glass insert for derivitisation. Seventy microlitres of borate buffer was then added to each sample and subsequently mixed prior to the addition of 20 μL of 3 mg/mL (w/v) AQC. Derivitisation reaction occurred with heating at 55°C for 10 min with gentle agitation at 750
rpm. Separation of samples was achieved using an Agilent 1200 Series LC system with a Binary SL pump (Agilent Technologies, California, USA). AQC derivatives were subsequently detected using an Agilent 6460 Triple Quad LC/MS with dynamic MRM detection. All samples were analysed using Agilent Masshunter Quantitative Analysis software, version B.03.02, 2008 (Agilent Technologies). Plasma amino acid concentrations were determined by measuring the absolute area under the curve relative to a standard curve generated using preparations of an internal standard (2-aminobutyric acid; data not shown). All samples from an individual subject were analysed within the same assay in a randomised order.
Immunoblotting
Approximately 30 mg of muscle was homogenized, in lysis buffer (pH 7.0) containing 20 mM Tris–HCl, 5 mM EDTA, 10 mM Na-pyrophosphate, 100 mM NaF, 2 mM Na3VO4, 1% Igepal, 10 g/mL Aprotinin, 10 g/mL Leupeptin, 3 mM Benzamidine and 1 mM PMSF.
The homogenate was rotated for 1 h at 4°C and subsequently centrifuged at 14,000 rpm for 15 min with the resulting supernatant collected into a fresh tube. Protein concentrations were determined using a BCA Protein Assay (Pierce, Thermo Scientific, New South Wales, Australia) with bovine serum albumin (BSA) as a standard. Muscle homogenate was then denatured in loading buffer containing dithiothreitol (DTT) and separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Proteins were then transferred onto nitrocellulose membrane and blocked at room temperature using 5% BSA (Sigma-Aldrich) in tris buffered saline with 0.1% (v/v) Tween-20 (TBST; Sigma-Aldrich). Membranes were incubated overnight with antibodies in 5% BSA at 4°C. Expression of signalling kinases was determined using primary antibodies (1:1,000) specific for IRS1Tyr612, AktSer473, mTORSer2448, p70S6KThr389, and ribosomal S6Ser240/244 (Cell Signaling). Membranes were subsequently washed and then incubated with anti-rabbit HRP-conjugated secondary antibody (1:1000; Calbiochem) for 1 h at room temperature. Following this, membranes were washed repeatedly as before, and proteins visualized using enhanced chemiluminescence (Perkin-Elmer, Queensland, Australia) on a Kodak 4000MM Image Station (Kodak, New York, USA) using a CCD camera. Membranes were stripped using Restore Western Blot Stripping buffer™ (Quantum Scientific, Victoria, Australia) and then subsequently reprobed for total mTOR, total p70S6K, and total eIF4G proteins to verify the relative amount of analyzed proteins. Band density was quantified using Kodak imaging software version 4.5.0 (Kodak); each phosphorylated protein was normalized to its respective total protein.
Statistical analysis
Statistical analysis was performed using SPSS version 20 for Windows (SPSS Inc.). Data are reported as means ± standard error of the mean (SEM). Paired Students t-test with Bonferroni adjustment was used to determine significance of between group differences at baseline. A two-way analysis of variance (ANOVA) with group (control, MetS) and meal (dairy, soy) as factors was used to test for BCAA area under the curve (AUC) difference. Differences in all other variables were tested using a three-way ANOVA with group (control, MetS) and meal (dairy, soy) as between subject factors and time as a within subject factor. The Holm-Sidak post hoc method was used to compare pair wise differences when interactions were present. A probability level of <0.05 was adopted throughout to determine statistical significance.
Discussion
The primary finding of the present study is that obese middle-aged men with metabolic syndrome but without overt type 2 diabetes show impairment in anabolic signalling following the consumption of ~30 g of protein in the context of a mixed-meal. This impairment was evidenced by a lack of p70S6K activation at 2 and 4 hours after mixed-meal consumption compared to healthy control subjects. No differences between subjects with MetS and healthy controls were observed in the activation of ribosomal protein S6 which is also a downstream target within the mTOR pathway. S6 activation and mTOR Ser2448 activation were greater following the ingestion of the dairy meal compared with the soy meal suggesting that the more sustained levels of BCAAs in the plasma following consumption of the dairy meal may result in a greater anabolic stimulus than the soy meal.
We report that when matched for total protein, fat, and similar in carbohydrate content dairy and soy based meal result in very similar blood glucose and insulin responses but that the dairy meal results in a more sustained BCAA concentration in the plasma and a larger BCAA AUC. The more sustained BCAA response is likely a result of the greater BCAA content in dairy meal and a slower release from the gut. Despite not being overtly diabetic the MetS group displayed a much larger insulin response and slightly greater glucose response to both meals compared with the control subjects. This in combination with the difference in HOMA at baseline indicates large divergence in insulin sensitivity between groups. Notwithstanding the observed differences in insulin sensitivity between MetS and controls, there were no significant differences in IRS1 or Akt phosphorylation after the consumption of either meal in either controls or MetS. Previous work has shown that type 2 diabetics display an insulin resistance to amino acid infusion [
25]; however, in this study a mixed-meal does not appear to impair anabolic insulin signalling in the muscle of middle age men with MetS. It seems unlikely that insulin insensitivity is responsible for the observed downstream signalling deficit in the MetS group. However, it is possible that the addition of more subjects would have resulted in significant differences in Akt or IRS1 phosphorylation between controls and MetS. At 2 h post meal Akt phosphorylation was ~ two fold higher and IRS1 was ~ two fold lower in the controls compared to the MetS.
P70S6K is the primary readout used to assess the activity of the mTOR pathway [
6,
34]; the lack of change in P70S6K phosphorylation after mixed-meal consumption in men with MetS is similar to the anabolic resistance induced by periods of inactivity [
11,
12] and is observed in older adults [
9,
14]. Although protein synthesis was not directly measured in this study signalling deficits have been shown to underlie ageing induced anabolic resistance [
9]. In the current study we did not measure physical activity or physical fitness; however, it is likely that the MetS subjects were less physically active and fit than the controls [
35]. Recent work in obese older adults has shown an insensitivity of MPS to a mixed macronutrient beverage ingestion which is transiently reversed by energy deficit and weight loss; however, the anabolic sensitivity was lost once subjects returned to their normal unsupervised diet despite maintained weight loss [
36]. A possible mechanism which could underlie this anabolic resistance may be higher baseline resting phosphorylation of mTOR pathway components such as p70S6K [
37]. This could be due to a constant nutrient excess in obese subjects [
38,
39]. Other studies have shown higher levels of BCAAs in the obese even under fasting conditions [
40]; however, we did not replicate this finding. Katta
et al. (2009) showed diminished activation of Akt/mTOR kinases, including p70S6K, within the skeletal muscle of obese compared to lean rats following a contractile stimulus [
41]. Conversely, similar rates of protein synthesis were observed in the skeletal muscle of T2DM and control subjects following administration of a high-energy clamp [
42]. However, Nilsson
et al. (2010) have demonstrated that while cytosolic and myofibrillar proteins exhibit similar rates of protein synthesis in obese and lean rats, synthesis of mitochondrial proteins is blunted in the obese group [
43]. Collectively with the aforementioned studies, the current data suggests that perhaps metabolic disease results in a divergent anabolic response in skeletal muscle which is influenced by both metabolic irregularities and nutritional stimuli.
The greater S6 activation seen after the consumption of the dairy meal compared with the soy meal suggests that the greater BCAA content of dairy meal was sufficient to induce greater S6 activation. MetS did not affect S6 activation suggesting that S6 and p70S6K are regulated differently. At first it may seem surprising that mTOR phosphorylation was not increased in the healthy controls after the soy meal whereas its downstream target p70S6K was increased; however, the Ser2448 site on mTOR is actually an inhibitory site which is phosphorylated by p70S6K as a negative feedback mechanism [
44]. This suggests that prior to the first post meal muscle biopsy (2 h) there may have been transient activation of the mTOR complex as well as p70S6K which was diminished by the time of sampling in the MetS group.
There have been a number of studies which have looked at the effects of different proteins on muscle anabolism however; very little research has addressed the anabolic potential of different mixed meals more closely associated with those consumed in everyday life. A fundamental characteristic of the test meals used in the present study was a large fat content (54 g). Previous research indicates that a high fat diet leads to oversaturation of the oxidative capacity of mitochondria in muscle [
45]. It is unclear if the differences in mTOR and S6 activation in the present study are simply a result of different protein sources [
18] and leucine content or if there is an interaction between the fat or carbohydrate and protein source.
Although this study measured anabolic signalling throughout the mTOR pathway which controls the initiation of protein translation and thus protein synthesis, acute anabolic signalling measurements do not always perfectly line up with measurements of MPS or long term phenotypic change [
46,
47]. The measurement of anabolic signalling provides a ‘snapshot’ of the state of protein translation within the muscle; because measurements were made only 2 and 4 hours after meal consumption it is possible that activation prior to the first measurement or between the measurements could have been missed. Future work should directly measure MPS over time to provide an average MPS in the postprandial period.
There are many conflicting reports in the literature concerning anabolic resistance of protein synthesis and anabolic signalling deficits in ageing; even less is known about anabolic resistance or signalling deficits in middle aged men with obesity and metabolic syndrome. We report that MetS is associated with impaired downstream signalling in the mTOR pathway in response to two different high protein mixed meals. Secondarily, we report that other mTOR pathway targets are activated to a greater degree following dairy mixed consumption compared with a soy based meal matched for protein. The primary novel aspect of this study was the ingestion of a complete breakfast consisting of whole foods rather than an isolated liquid protein supplement. Future work should look to use measurements of muscle protein synthesis to directly assess differences in anabolic sensitivity in subjects with MetS using mixed meals rather than amino acid infusions and clamp methodology.
Acknowledgements
The authors would like to thank the study volunteers. The authors are also exceptionally grateful for the medical support supplied by Dr Andrew Garnham, who undertook all muscle biopsy procedures. The authors also wish to acknowledge the assistance of the Australian Proteome Analysis Facility staff for quantification of the test meals, which was facilitated using infrastructure provided by the Australian Government through the National Collaborative Research Infrastructure Strategy (NCRIS). This project was supported with funding from the Dairy Health and Nutrition Consortium (Bega Cheese/Tatura Milk Industries, Fonterra Australia, Lion Dairy and Drinks, Murray Goulburn Co-operative, Parmalat Australia, Warrnambool Cheese & Butter Factory, Geoffrey Gardiner Foundation, Dairy Australia, and Dairy Innovation Australia). There were no conflicts of interest.
Funding
This research was supported by funding from Dairy Health and Nutrition Consortium through project 08131D.
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Competing interests
The authors declare that they have no competing interests.
Authors’ contributions
The authors responsibilities were as follows; PG conducted the research, analysed the data. AL and ALD assisted with the research. MB was involved in the project conception and study oversight. TR and CS assisted with data analyses. AN and CM conducted the statistical analysis. DT contributed towards final content. AJS designed research and project conception. PG, CM and DCS wrote the manuscript. DCS designed research, project conception and had primary responsibility for final content. All authors read and approved the final manuscript.