Background
Case presentation
Clinical data
Case one
Case two
Histopathological and immunohistochemical methods
Antibody | Expression in SPC | Expression in IMT | Source/Dilution |
---|---|---|---|
First stainings | |||
Vimentin * |
++
|
++
| DAKO, mouse, 1:800 |
PanCytokeratin AE1/3 * |
+
|
NR
| DAKO, mouse, 1:100 |
EMA |
+
|
+ (weak only)
| LOXO, mouse, 1:20 |
ALK-1 * |
NR
|
+
| DAKO, mouse, 1:20 |
smooth muscle Actin |
++
|
NR
| Beckman Coulter, mouse, 1:20 |
Desmin |
NR
|
NR
| DAKO, mouse, 1:400 |
S100 |
NR
|
NR
| DAKO, rabbit, 1:2000 |
CD34 |
NR
|
NR
| DAKO, mouse, 1:100 |
CD117 |
NR
|
NR
| DAKO, mouse, 1:100 |
Ki67 * |
++ 60–80%
|
+ 5–10%
| DAKO, mouse, 1:200 |
Additional stainings | |||
PanCytokeratin KL1 * |
+
|
NR
| Beckman Coulter, mouse, 1:40 |
PanCytokeratin MNF116 |
NR (but epithelium +)
|
NR
| DAKO, mouse, 1:50 |
Cytokeratin 5/6 |
+ (weak only)
|
NR
| DAKO, mouse, 1:50 |
Cytokeratin 7 |
NR
|
NR
| DAKO, mouse, 1:20 |
CD68 |
NR
|
NR
| Kiel, ascites, 1:20000 |
CD30 |
NR
|
NR
| DAKO, mouse, 1:5 |
CD56 |
NR
|
NR
| DAKO, mouse, 1:10 |
Her2Neu |
NR
|
NR
| DAKO, rabbit, 1:100 |
Estrogen receptor |
NR
|
NR
| DAKO, mouse, 1:10 |
Progesteron receptor |
NR
|
NR
| DAKO, mouse, 1:10 |
p53 |
+
|
+
| DAKO, mouse, 1:20 |
p63 * |
++
|
NR
| Neomarkers, mouse, 1:200 |
p21 |
++
|
++
| Dianova, rat, 1:20 |
Cyclin D1 |
+
|
+
| LOXO, mouse, 1:20 |
Bcl-2 |
+ (weak cytoplasmatic)
|
++
| DAKO, mouse, 1:400 |
Rb |
+
|
++
| Neomarkers, mouse 1:50 |
HHV 8 |
NR
|
NR
| Tebu, rat, 1:200 |
HPV |
NR
|
NR
| Virofem, mouse conc. |
Results
Case one
Case two
Conclusion
Patient, Age | Symptoms | Site | CS | Course | Immunostaining |
---|---|---|---|---|---|
M, 62 [20] | Hoarseness, dysphonia, systemic manifestations (fever and weight loss) for 3 months | True vocal cord | yes | FD 24 months | vim +, actin +, NR: desmin, CK, S100, Alk-1 |
M, 2 [9] | Snoring, apneic events | Aryepiglottic fold | no | R 7 months | * |
F, 52 [11] | Voice change for 12 months | False cord | no | FD 4 years | vim +, sm-actin +, NR: S100, desmin |
F, 49 [10] | Dyspnoea, stridor for 4 month, 15 month after road accident | Subglottis | no | Persistence for 1 months after surgery, resurgery with persistence for 12 months, 2nd resurgery, FD 15 months after resurgery | sm-actin patchy +, NR: desmin, CK, EMA |
M, 51 [32] | Hoarseness for 6 months | Vocal cord | * | FD 12 months | vim+, sm-actin+, NR: CK, desmin, S100, CD34 |
M, 74 [33] | Hoarseness, foreign body sensation, dyspnoe for several months | True vocal cord | * | FD 2 months, death on cardiac failure | vim +, actin +, NR: S100, desmin, CK |
M, 57 [34] | Hoarseness, dyspnoe, disphonia for 5 months | Anterior commissur | * | R 2 months, after resurgery FD 12 months | vim +, actin + |
M, 72 [35] | Dysphonia for 2 months | True vocal cord | * | FD 6 months | vim +, sm-actin +, CD68 focally +, NR: CK, S100, desmin, CD34, LMP |
5 M, 3 F with age median of 59 (19–69) [8] | Hoarseness, stridor, dysphonia, foreign body sensations from 10 days up to 4 months | * | 3+/8 | 7 cases FD, one case with 4 relapses in 24 months | All: vim +, sm-actin +, NR: CK, S100, desmin, myoglobin, CD34 |
3 further cases, [7] | * | * | * | 2 cases FD, one R 12 months | * |