Background
Methods
Sample collection
Characteristics | Tissue | Serum | Urine |
---|---|---|---|
Gastric cancer
| 12 | 12 | 90 |
Mean age (range) | 60(42–76) | 63(84–39) | 60(31–85) |
Gender | |||
Male | 6 | 7 | 67 |
Female | 6 | 5 | 23 |
Tumor location | |||
Cardia | 3 | 2 | 39 |
Body | 5 | 6 | 26 |
Antrum | 3 | 4 | 16 |
Diffuse | 1 | 0 | 9 |
Operation | |||
Total Gastrectomy | 4 | 5 | 29 |
Subtotal Gastrectomy | 8 | 7 | 61 |
Lauren classification | |||
Intestinal | 5 | 4 | 39 |
Diffuse | 7 | 8 | 51 |
Histology type | |||
High or moderate | 5 | 5 | 43 |
Poor or undifferentiated | 7 | 7 | 47 |
Tumor stages | |||
Ι and ΙΙ | 2 | 4 | 31 |
ΙΙΙ and ΙV | 10 | 8 | 59 |
Depth of invasion | |||
T1 and T2 | 5 | 9 | 30 |
T3 and T4 | 7 | 3 | 60 |
Node status | |||
N0 | 3 | 3 | 29 |
N1 and N2 | 9 | 9 | 61 |
Metastasis status | |||
M0 | 5 | 10 | 71 |
M1 | 7 | 2 | 19 |
Lung cancer
| - | - | 9 |
Mean age (range) | 46(42–57) | ||
Colon cancer
| - | - | 10 |
Mean age (range) | 53(46–65) | ||
Rectum cancer
| - | - | 9 |
Mean age (range) | 62(47–78) | ||
Healthy
| 12 | 12 | 57 |
Mean age (range) | 60(42–76) | 54(41–72) | 47 (29–76) |
Gastritis/Chronic inflammation
| - | - | 2 |
Mean age (range) | 56 (43–69) | ||
Gastritic leiomyoma
| 2 | ||
Mean age (range) | - | - | 56 (51–62) |
Western blotting analysis
Immunohistochemistry
Statistical analysis
Results
EL expression levels in urine
Clinicopathological (n) | EL absent (%) | EL present (%) |
P
|
---|---|---|---|
n = 71 | n = 19 | ||
Gender | |||
Male, (67) | 51 (72) | 16 (84) | 0.27 |
Female, (23) | 20 (28) | 3 (16) | |
Histological type | |||
High or moderate, (43) | 31 (44) | 12 (63) | 0.13 |
Poor or undifferentiated, (47) | 40 (56) | 7 (37) | |
Lauren classification | |||
Intestinal, (39) | 29 (41) | 10 (53) | 0.36 |
Diffuse, (51) | 42 (59) | 9 (47) | |
Tumor stages | |||
Ι and ΙΙ, (31) | 22 (31) | 9 (47) | 0.18 |
ΙΙΙ and ΙV, (59) | 49 (69) | 10 (53) | |
Depth of invasion | |||
T1 and T2, (30) | 21 (30) | 9 (47) | 0.14 |
T3 and T4, (60) | 50 (70) | 10 (53) | |
Node status | |||
Absent, (29) | 23 (32) | 6 (32) | 0.95 |
Present, (61) | 48 (68) | 13 (68) | |
Metastasis status | |||
M0 (71) | 55 (77) | 16 (84) | 0.52 |
M1 (19) | 16 (23) | 3 (16) |
EL expression levels in tissues and sera
n
| The expression of EL protein |
P
| ||
---|---|---|---|---|
Negative | Positive | |||
Adjacent control
| 12 | 4(33%) | 8 (67%) | 0.67 |
Gastric cancer
| 12 | 5(42%) | 7 (58%) |
Clinicopathological |
n
| EL |
P
| |
---|---|---|---|---|
Negative | Positive | |||
Gender | ||||
Male | 6 | 2 | 4 | 0.56 |
Female | 6 | 3 | 3 | |
Age (year) | ||||
≤50 | 3 | 1 | 2 | 0.74 |
>50 | 9 | 4 | 5 | |
Lauren classification | ||||
Intestinal | 5 | 3 | 2 | 0.28 |
Diffuse | 7 | 2 | 5 | |
Histological type | ||||
Highly differentiated | 1 | 1 | 0 | 0.36 |
Moderately differentiated | 4 | 2 | 2 | |
Poorly differentiated | 7 | 2 | 5 | |
Tumor stages | ||||
I and II | 2 | 1 | 1 | 0.79 |
III and IV | 10 | 4 | 6 | |
Depth of invasion | ||||
T1 and T2 | 5 | 2 | 3 | 0.92 |
T3 and T4 | 7 | 3 | 4 | |
Lymph node metastasis | ||||
Positive | 3 | 1 | 2 | 0.74 |
Negative | 9 | 4 | 5 |