Background
Methods
Study population and data collection
IgG capturing and N-glycan release
Sialic acid derivatization and HILIC enrichment
MALDI-TOF-MS and data processing
Statistical analysis
Results
Response during pregnancy and flare after delivery
Fab glycosylation is higher in RA patients outside pregnancy when compared to controls, yet with similar glycans
Healthy | RA | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
3rd trima
| 26 wkpp | pre-conception | 3rd trim | 26 wkpp | ||||||
Median (%) | IQR | Median (%) | IQR | Median (%) | IQR | Median (%) | IQR | Median (%) | IQR | |
Fc | ||||||||||
Galactosylation | 81.5 | [78.1–83.2] | 71.6 | [67.9–74.7] | 60.8 | [52.2–64.3] | 73.6 | [66.5–79.5] | 61.0 | [56.8–66.7] |
Sialylation | 18.4 | [17.1–20.5] | 13.1 | [11.5–15.8] | 11.0 | [9.0–13.1] | 16.0 | [14.0–19.7] | 11.5 | [9.4–14.1] |
Fucosylation | 96.8 | [95.8–97.4] | 97.3 | [96.6–98.0] | 97.9 | [97.5–98.3] | 97.5 | [96.5–98.0] | 97.7 | [97.2–98.2] |
Bisection | 8.7 | [7.3–9.7] | 9.2 | [8.3–10.5] | 10.3 | [9.0–11.5] | 8.6 | [7.4–10.2] | 11.0 | [9.5–13.4] |
Total | ||||||||||
Galactosylation | 82.9 | [80.6–84.6] | 74.8 | [70.3–77.0] | 66.0 | [59.9–69.0] | 77.5 | [73.5–82.2] | 68.6 | [59.1–73.5] |
Sialylation | 26.2 | [23.9–28.7] | 21.3 | [19.4–22.6] | 20.8 | [19.1–23.8] | 27.3 | [24.4–28.4] | 22.6 | [20.0–24.5] |
Fucosylation | 95.4 | [94.7–96.2] | 96.5 | [95.5–97.2] | 96.9 | [96.2–97.3] | 96.2 | [95.4–96.7] | 96.7 | [96.0–97.1] |
Bisection | 12.6 | [10.6–14.1] | 14.8 | [13.3–17.3] | 18.1 | [15.8–19.8] | 15.2 | [13.6–17.7] | 18.1 | [16.8–19.6] |
Fab | ||||||||||
Galactosylation | 96.8 | [96.3–97.0] | 97.2 | [96.5–97.8] | 96.6 | [95.7–97.7] | 97.3 | [96.9–97.8] | 96.8 | [96.0–97.3] |
Sialylation | 81.6 | [80.4–84.3] | 79.0 | [77.8–82.6] | 79.7 | [77.0–81.1] | 82.7 | [80.6–84.6] | 80.2 | [76.9–81.6] |
Fucosylation | 70.6 | [68.0–75.3] | 76.6 | [74.5–80.6] | 75.6 | [71.9–80.5] | 73.2 | [61.6–75.8] | 77.7 | [72.8–79.7] |
Bisection | 37.0 | [36.1–41.4] | 46.6 | [41.7–52.2] | 51.5 | [46.5–55.2] | 42.1 | [33.8–44.4] | 52.2 | [46.7–54.6] |
% glycosylation | 17.6 | [13.7–21.0] | 16.5 | [13.3–18.2] | 22.0 | [18.7–27.4] | 28.0 | [23.3–32.7] | 21.4 | [19.5–25.4] |
Healthy | RA | ||||
---|---|---|---|---|---|
3rd trima
| 26 wkpp | pre-conc. | 3rd trim | 26 wkpp | |
Fc | |||||
Galactosylation | 29 | 25 | 33 | 31 | 33 |
Sialylation | 29 | 25 | 33 | 31 | 33 |
Fucosylation | 29 | 25 | 33 | 31 | 33 |
Bisection | 29 | 25 | 33 | 31 | 33 |
Total | |||||
Galactosylation | 25 | 25 | 33 | 30 | 31 |
Sialylation | 25 | 25 | 33 | 30 | 31 |
Fucosylation | 25 | 25 | 33 | 30 | 31 |
Bisection | 25 | 25 | 33 | 30 | 31 |
Fab | |||||
Galactosylation | 17 | 15 | 29 | 25 | 23 |
Sialylation | 17 | 15 | 29 | 25 | 23 |
Fucosylation | 17 | 15 | 29 | 25 | 23 |
Bisection | 17 | 15 | 29 | 25 | 23 |
% glycosylation | 15 | 15 | 29 | 24 | 21 |
RA vs. healthy at 26 wkppa
| Healthy | RA | ||
---|---|---|---|---|
3rd trim vs. 26 wkpp | pre-conc. vs. 3rd trim | 3rd trim vs. 26 wkpp | ||
Fc | ||||
Galactosylation |
0.000
|
0.000
|
0.000
|
0.000
|
Sialylation | 0.077 |
0.000
|
0.000
|
0.000
|
Fucosylation | 0.072 |
0.000
| 0.003 | 0.048 |
Bisection | 0.004 | 0.003 |
0.000
|
0.000
|
Total | ||||
Galactosylation |
0.001
|
0.000
|
0.000
|
0.000
|
Sialylation | 0.179 |
0.000
|
0.000
|
0.000
|
Fucosylation | 0.889 |
0.000
|
0.000
| 0.012 |
Bisection |
0.000
|
0.000
|
0.000
|
0.000
|
Fab | ||||
Galactosylation | 0.347 | 0.333 | 0.004 | 0.148 |
Sialylation | 0.754 | 0.028 |
0.000
| 0.007 |
Fucosylation | 0.709 | 0.005 |
0.000
| 0.003 |
Bisection | 0.104 | 0.005 |
0.000
|
0.001
|
% glycosylation |
0.002
| 0.401 | 0.009 | 0.249 |