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01.05.2013 | Scientific Article

Magnetic resonance imaging and histology of ovine hip joint cartilage in two age populations: a sheep model with assumed healthy cartilage

verfasst von: Christoph Zilkens, Falk R. Miese, Clemens Crumbiegel, Young-Jo Kim, Monika Herten, Gerald Antoch, Rüdiger Krauspe, Bernd Bittersohl

Erschienen in: Skeletal Radiology | Ausgabe 5/2013

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Abstract

Objective

To compare morphologically normal appearing cartilage in two age groups with delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and correlate magnetic resonance imaging (MRI) findings with histology.

Materials and methods

Twenty femoral head specimens collected from ten lambs (group I) and ten young adult sheep (group II) underwent dGEMRIC and histological assessment. A region of 2 cm² with morphologically normal-appearing cartilage was marked with a surgical suture for subsequent matching of MRI and histological sections. The MRI protocol included a three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment, a B1 pre-scan with various flip angles for B1 field heterogeneity correction, and 3D volumetric interpolated breathhold examination for T1_Gd mapping (dGEMRIC). Histological analysis was performed according to the Mankin scoring system.

Results

A total of 303 regions of interest (ROI; 101 MRI reformats matching 101 histological sections) was assessed. Twenty-six ROIs were excluded owing to morphologically apparent cartilage damage or insufficient MR image quality. Therefore, 277 ROIs were analyzed. Histological analyses revealed distinct degenerative changes in various cartilage samples of group II (young adult sheep). Corresponding T1_Gd values were significantly lower in the group of sheep (mean T1_Gd = 540.4 ms) compared with the group of lambs (mean T1_Gd = 623.6 ms; p < 0.001).

Conclusions

Although morphologically normal, distinct cartilage degeneration may be present in young adult sheep cartilage. dGEMRIC can reveal these changes and may be a tool for the assessment of early cartilage degeneration.

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Titel: Magnetic resonance imaging and histology of ovine hip joint cartilage in two age populations: a sheep model with assumed healthy cartilage
verfasst von: Christoph Zilkens
Falk R. Miese
Clemens Crumbiegel
Young-Jo Kim
Monika Herten
Gerald Antoch
Rüdiger Krauspe
Bernd Bittersohl
Publikationsdatum: 01.05.2013
Verlag: Springer-Verlag
Erschienen in: Skeletal Radiology / Ausgabe 5/2013
Print ISSN: 0364-2348
Elektronische ISSN: 1432-2161
DOI: https://doi.org/10.1007/s00256-012-1554-7

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Magnetic resonance imaging and histology of ovine hip joint cartilage in two age populations: a sheep model with assumed healthy cartilage