Erschienen in:
01.07.2009 | Original Article
Plasminogen fragment K1–3 inhibits expression of adhesion molecules and experimental HCC recurrence in the liver
verfasst von:
Esther Raskopf, Sevil Gerceker, Annabelle Vogt, Jens Standop, Tilman Sauerbruch, Volker Schmitz
Erschienen in:
International Journal of Colorectal Disease
|
Ausgabe 7/2009
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Abstract
Purpose
There is no established adjuvant or neo-adjuvant treatment to curb tumor recurrence of hepatocellular carcinoma (HCC). Recent data showed that angiostatic factors can inhibit tumor cell adhesion to the endothelium and therefore recurrence/metastasis. We tested a potential preventive, pre-operative strategy using plasminogen kringles 1–3 (K1–3) to overcome this hurdle.
Materials and methods
Effects of K1–3 on the intercellular cell adhesion molecule (ICAM) and vascular cell adhesion molecule (VCAM) expression was analyzed in vitro and in vivo on RNA and protein levels. Influence of K1–3 on HCC recurrence in the liver was analyzed in an orthotopic tumor model.
Results
K1–3 decreased ICAM expression in Hepa129 tumor cells and VCAM expression in SVEC4-10 endothelial cells in vitro. In vivo, ICAM was reduced in histological tumor sections. Preventive treatment with AdK1–3 inhibited experimental HCC recurrence and tumor growth in the liver.
Conclusions
We were able to show that K1–3 inhibits intrahepatic tumor recurrence. This novel aspect elucidates a possible approach to prevent HCC recurrence.