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Digestive Diseases and Sciences

Erschienen in:

01.09.2007 | Original Article

Polymeric Formula Has Direct Anti-Inflammatory Effects on Enterocytes in an in VitroModel of Intestinal Inflammation

verfasst von: Naomi S. H. de Jong, Steven T. Leach, Andrew S. Day

Erschienen in: Digestive Diseases and Sciences | Ausgabe 9/2007

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Abstract

Exclusive enteral nutrition using polymeric formula (PF) is a well-established therapeutic option for active Crohn’s disease; however, its mechanisms of action are unknown. We investigated the anti-inflammatory effects of PF in an in vitro model of epithelial cell inflammation. PF did not affect cell viability over a range of dilutions, but when PF was added to the culture medium the interleukin (IL)-8 response to proinflammatory stimuli was significantly reduced. This effect was due to PF acting directly on the cells as the IL-8 response was still reduced when PF was separated from the proinflammatory stimuli in a 2-compartment system. In the presence of PF, nuclear factor (NF)-κB nuclear migration was not inhibited; however, IκBα degradation was delayed. PF has direct anti-inflammatory effects upon immortalized colonic enterocytes. Therefore PF may, in part, modulate gut inflammation by directly reducing the inflammatory response of the intestinal epithelium.

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Titel: Polymeric Formula Has Direct Anti-Inflammatory Effects on Enterocytes in an in VitroModel of Intestinal Inflammation
verfasst von: Naomi S. H. de Jong
Steven T. Leach
Andrew S. Day
Publikationsdatum: 01.09.2007
Verlag: Springer US
Erschienen in: Digestive Diseases and Sciences / Ausgabe 9/2007
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-006-9449-x

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Polymeric Formula Has Direct Anti-Inflammatory Effects on Enterocytes in an in VitroModel of Intestinal Inflammation

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