Erschienen in:
17.03.2022 | Short Communication
Quantification of MDMB-4en-PINACA and ADB-BUTINACA in human hair by gas chromatography–tandem mass spectrometry
verfasst von:
Yue Wang, Yefei Pan, Hongkun Yang, Jinlei Liu, Amin Wurita, Koutaro Hasegawa
Erschienen in:
Forensic Toxicology
|
Ausgabe 2/2022
Einloggen, um Zugang zu erhalten
Abstract
Purpose
To test synthetic cannabinoid (SCs) in parent forms from living human, the hairs seems to be one of the best samples, because of the non-invasiveness upon their collection. The purpose of this study is to establish a method for quantification of MDMB-4en-PINACA and ADB-BUTINACA, the most recently abused SCs in hair samples, using gas chromatography−tandem mass spectrometry (GC−MS/MS).
Methods
The collected hair samples were washed with a detergent solution, following by water and acetone. After drying cutting them into about 2 mm sections, they were ground by a cryogenic grinder into powder. The 50-mg powder with internal standard(s) plus 1 mL methanol were vortexed, and centrifuged to obtain the supernatant layer. After its evaporation and reconstitution with 50 µL methanol, 1-µL aliquot of it was subjected to analysis.
Results
The standard calibration curves were created for both MDMB-4en-PINACA and ADB-BUTINACA in blank hair samples; good linear curves were obtained in the range of 20–20,000 pg/mg with correlation coefficients greater than 0.99. The limits of detection and limits of quantification were 10 and 20 pg/mg, respectively. Other validation parameters were all satisfactory. The concentrations of MDMB-4en-PINACA obtained from 3 authentic subjects and ADB-BUTINACA obtained from 3 authentic subjects were 26.2–806 pg/mg and 63.1–430 pg/mg, respectively.
Conclusions
In the present article, the details of simple and rapid quantification of MDMB-4en-PINACA and ADB-BUTINACA in human scalp hair have been established. To our knowledge, this is the first report for quantification of SCs in hair samples by GC−MS/MS.