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08.11.2018 | Original Article

Radiation Therapy Reduced Blood Levels of LDH, HIF-1α, and miR-210 in OSCC

verfasst von: Marcela Gonçalves de Souza, Sabrina Ferreira de Jesus, Eloá Mangabeira Santos, Emisael Stenio Batista Gomes, Arlen de Paulo Santiago Filho, Eliane Macedo Sobrinho Santos, Luiz Henrique da Silveira, Sérgio Henrique Sousa Santos, Alfredo Maurício Batista de Paula, Lucyana Conceição Farias, André Luiz Sena Guimarães

Erschienen in: Pathology & Oncology Research | Ausgabe 1/2020

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Abstract

Radiation Therapy (RT) is a treatment option for a large number of neoplasias. However, the effect of RT on the level of hypoxia markers is poorly understood. The present study aimed to investigate the effect of RT on the levels of hypoxic markers in Oral squamous cell carcinoma (OSCC). Evaluation of HIF-1α and miR-210 levels in OSCC was performed. Then a proteomic analysis was performed to identify candidate hypoxic targets of RT. To validate proteomic studies, the effect of RT on HIF-1α, miR-210, PDH-A and LDH-A levels under hypoxia was assessed by qRT-PCR. The impact of RT in hypoxia markers was evaluated in patients to confirm in vitro results. An increase in the HIF-1α levels was observed in OSCC. RT reduced OSCC cell proliferation and migration. Interestingly, hypoxia could revert the effect of radiation on OSCC phenotype. However, proteomics analyses suggested that LDH is one of the critical targets of RT even in hypoxia. Moreover, RT decreased HIF-1α, miR-210, and LDH even in hypoxia. The current study demonstrated that hypoxia could revert the effects of RT in the OSCC context. However, RT reduces the levels HIF-1α, miR-210 and LDH in vivo and in vitro. The consequences of RT in blood should be carefully investigated.

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Titel: Radiation Therapy Reduced Blood Levels of LDH, HIF-1α, and miR-210 in OSCC
verfasst von: Marcela Gonçalves de Souza
Sabrina Ferreira de Jesus
Eloá Mangabeira Santos
Emisael Stenio Batista Gomes
Arlen de Paulo Santiago Filho
Eliane Macedo Sobrinho Santos
Luiz Henrique da Silveira
Sérgio Henrique Sousa Santos
Alfredo Maurício Batista de Paula
Lucyana Conceição Farias
André Luiz Sena Guimarães
Publikationsdatum: 08.11.2018
Verlag: Springer Netherlands
Erschienen in: Pathology & Oncology Research / Ausgabe 1/2020
Print ISSN: 1219-4956
Elektronische ISSN: 1532-2807
DOI: https://doi.org/10.1007/s12253-018-0517-2

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Radiation Therapy Reduced Blood Levels of LDH, HIF-1α, and miR-210 in OSCC

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