nach oben

Metabolic Brain Disease

Erschienen in:

01.12.2010 | Original Paper

Role of inducible or neuronal nitric oxide synthase in neurogenesis of the dentate gyrus in aged mice

verfasst von: Minami Adachi, Manami Abe, Taeko Sasaki, Hiroyuki Kato, Jiro Kasahara, Tsutomu Araki

Erschienen in: Metabolic Brain Disease | Ausgabe 4/2010

Einloggen, um Zugang zu erhalten

Abstract

We evaluated mainly the iNOS (inducible nitric oxide synthase) and nNOS (neuronal NOS) expression in the subgranular zone (SGZ) of the dentate gyrus of the hippocampus in young adult (8-week-old) and aged (60-week-old) mice. The present study demonstrates that the expression of nNOS was more pronounced than that of iNOS expression in the dentate gyrus of aged mice. Our study also suggests that aged mice exhibited a significant loss of motor activity as compared with young adult animals. Furthermore, our results provide that no significant change in the number of Neu N (Neuronal nuclei)-immunopositive neurons and GFAP (glial fibrillary acidic protein)—immunopositive astrocytes was observed in the dentate gyrus between young adult and aged mice. In contrast, a significant change in the number of Iba 1(ionized calcium-binding adaptor molecule 1)—immunopositive microglia in aged mice was observed in the dentate gyrus as compared to young adult animals. These results provide the novel evidence showing that the expression of nNOS may be crucial for the role of neurogenesis of the SGZ of the dentate gyrus in aged mice. Furthermore, our present findings demonstrate that the inhibition of nNOS expression in the SGZ of the dentate gyrus during aging processes may offer novel therapeutic strategies for anti-aging in humans.

Alvarez-Buylla A, Garcia-Verdugo JM (2002) Neurogenesis in adult subventricular zone. J Neurosci 22:629–634PubMed

Aoki E, Yokoyama H, Kimoto H, Yano R, Kato H, Araki T (2009) Chronic administration with rotenone does not enhance MPTP neurotoxicity in C57BL/6 mice. J Mol Neurosci 41:17–24CrossRefPubMed

Calhoun ME, Kurth D, Phinney AL, Long JM, Hengemihle J, Mouton PR, Ingram DK, Jucker M (1998) Hippocampal neuron and synaptophysin-positive bouton number in aging C57BL/6 mice. Neurobiol Aging 19:599–606CrossRefPubMed

Cheng A, Wang S, Cai J, Rao MS, Mattson MP (2003) Nitric oxide acts in a positive feedback loop with BDNF to regulate neural progenitor cell proliferation and differentiation in the mammalian brain. Dev Biol 258:319–333CrossRefPubMed

Dalkara T, Moskowitz MA (1994) The complex role of nitric oxide in the pathophysiology of focal cerebral ischemia. Brain Pathol 4:49–57CrossRefPubMed

David JP, Ghozali F, Fallet-Bianco C, Wattez A, Delaine S, Boniface B, Di Menza C, Delacourte A (1997) Glial reaction in the hippocampal formation is highly correlated with aging in human brain. Neurosci Lett 235:53–56CrossRefPubMed

Dun NJ, Dun SL, Wong RKS, Forstermann U (1994) Colocalization of nitric oxide synthase and somatostatin immunoreactivity in rat dentate hilar neurons. Proc Natl Acad Sci USA 91:2955–2959CrossRefPubMed

Eriksson PS, Perfilieva E, Bjork-Eriksson T, Alborn AM, Nordborg C, Peterson DA, Gage FH (1998) Neurogenesis in the adult human hippocampus. Nat Med 4:1313–1317CrossRefPubMed

Eto R, Abe M, Hayakawa N, Kato H, Araki T (2008) Age-related changes of calcineurin and Akt1/protein kinase Bα (AKt1/PKBα) immunoreactivity in the mouse hippocampal CA1 sector: an immunohistochemical study. Metab Brain Dis 23:399–409CrossRefPubMed

Finch CE (2002) Neurons, glia, and plasticity in normal brain aging. Adv Gerontol 10:35–39PubMed

Gage FH (2000) Structural plasticity: cause, result, or correlate of depression. Biol Psychiatry 48:713–714CrossRefPubMed

Hayakawa N, Kato H, Araki T (2007) Age-related changes of astorocytes, oligodendrocytes and microglia in the mouse hippocampal CA1 sector. Mech Ageing Dev 128:311–316CrossRefPubMed

Hayakawa N, Abe M, Eto R, Kato H, Araki T (2008) Age-related changes of NGF, BDNF, parvalbumin and neuronal nitric oxide synthase immunoreactivity in the mouse hippocampal CA1 sector. Metab Brain Dis 23:199–211CrossRefPubMed

Homburger F, Russfield AB, Weisburger JH, Lim S, Chak SP, Weisburger EK (1975) Aging changes in CD-1 HaM/ICR mice reared under standard laboratory conditions. J Natl Cancer Inst 55:37–45PubMed

Jin K, Minami M, Lan JQ, Mao XO, Batteur S, Simon RP, Greenberg DA (2001) Neurogenesis in the dentate subgranular zone and rostral subventricular zone after focal cerebral ischemia in the rat. Proc Natl Acad Sci USA 98:4710–4715CrossRefPubMed

Jin K, Sun Y, Xie L, Peel A, Mao XO, Batteur S, Greenberg DA (2003) Directed migration of neuronal precursors into the ischemic cerebral cortex and striatum. Mol Cell Neurosci 24:171–189CrossRefPubMed

Keynes RG, Garthwaite J (2004) Nitric oxide and its role in ischaemic brain injury. Curr Mol Med 4:179–191CrossRefPubMed

Kimelberg HK, Norenberg MD (1989) Astrocytes. Sci Am 260:66–72CrossRefPubMed

Kimoto H, Eto R, Abe M, Kato H, Araki T (2009) Alterations of glial cells in the mouse hippocampus during postnatal development. Cell Mol Neurobiol 29:1181–1189CrossRefPubMed

Kornack DR, Rakic P (1999) Continuation of neurogenesis in the hippocampus of the adult macaque monkey. Proc Natl Acad Sci USA 96:5768–5773CrossRefPubMed

Leker RR, Lasri V, Chernoguz D (2009) Growth factors improve neurogenesis and outcome after focal cerebral ischemia. J Neural Transm 116:1397–1402CrossRefPubMed

Long JM, Kalehua AN, Muth NJ, Calhoun ME, Jucker M, Hengemihle JM, Ingram DK, Mouton PR (1998) Stereological analysis of astrocyte and microglia in aging mouse hippocampus. Neurobiol Aging 19:497–503CrossRefPubMed

Matsuda S, Umeda M, Kato H, Araki T (2009) Glial damage after transient focal cerebral ischemia in rats. J Mol Neurosci 38:220–226CrossRefPubMed

Moreno-Lopez B, Romero-Grimaldi C, Noval JA, Murillo-Carretero M, Matarredona ER, Estrada C (2004) Nitric oxide is physiological inhibitor of neurogenesis in the adult mouse subventricular zone and olfactory bulb. J Neurosci 24:85–95CrossRefPubMed

Mouton PR, Long JM, Lei DL, Howard V, Jucker M, Calhoun ME, Ingram DK (2002) Age and gender effects on microglia and astrocyte numbers in brains of mice. Brain Res 956:30–35CrossRefPubMed

Nithianantharajah J, Hannan AJ (2009) The neurobiology of brain and cognitive reserve: mental and physical activity as modulators of brain disorders. Prog Neurobiol 89:369–382CrossRefPubMed

O’Callaghan JP, Miller DB (1991) The concentration of glial fibrillary acidic protein increases with age in the mouse and rat brain. Neurobiol Aging 12:171–174CrossRefPubMed

Ookubo M, Yokoyama H, Kato H, Araki T (2009) Gender differences on MPTP (1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine) neurotoxicity in C57BL/6 mice. Mol Cell Endocrinol 311:62–68CrossRefPubMed

Packer MA, Stasiv Y, Benraiss A, Chmielnicki E, Grinberg A, Westphal H, Goldman SA, Enikolopov G (2003) Nitric oxide negatively regulates mammalian adult neurogenesis. Proc Natl Acad Sci USA 100:9566–9571CrossRefPubMed

Parent JM, Vexler ZS, Gong C, Derugin N, Ferriero DM (2002) Rat forebrain neurogenesis and striatal neuron replacement after focal stroke. Ann Neurol 52:802–813CrossRefPubMed

Park C, Sohn Y, Shin KS, Kim J, Ahn H, Huh Y (2003) The chronic inhibition of nitric oxide synthase enhances cell proliferation in the adult rat hippocampus. Neurosci Lett 399:9–12CrossRef

Rietze R, Poulin P, Weiss S (2000) Mitotically active cells that generate neurons and astrocytes are present in multiple regions of the adult mouse hippocampus. J Comp Neurol 424:397–408CrossRefPubMed

Sasaki T, Hamada J, Shibata M, Araki N, Fukuuchi Y (2001) Inhibition of nitric oxide production during global ischemia ameliorates ischemic damage of pyramidal neurons in the hippocampus. Keio J Med 50:182–187PubMed

Sloane JA, Hollander W, Rosene DL, Moss MB, Kemper T, Abraham CR (2000) Astrocytic hypertrophy and altered GFAP degradation with age in subcortical white matter of the rhesus monkey. Brain Res 862:1–10CrossRefPubMed

Sun Y, Jin K, Childs JT, Xie L, Mao XO, Greenberg DA (2005) Neuronal nitric oxide synthase and ischemia-induced neurogenesis. J Cereb Blood Flow Metab 25:485–492CrossRefPubMed

Yokoyama H, Kuroiwa H, Tsukada T, Uchida H, Kato H, Araki T (2010) Poly (ADP- ribose)polymerase inhibitor can attenuate the neuronal death after 1-methyl-4- phenyl-1, 2, 3, 6-tetrahydropyridine-induced neurotoxicity in mice. J Neurosci Res 88:1522–1536PubMed

Zhu DY, Liu SH, Sun HS, Lu YM (2003) Expression of inducible nitric oxide synthase after focal cerebral ischemia stimulates neurogenesis in the adult rodent dentate gyrus. J Neurosci 23:223–229PubMed

Zhu XJ, Hua Y, Jiang J, Zhou QG, Luo CX, Han X, Lu YM, Zhu DY (2006) Neuronal nitric oxide synthase-derived nitric oxide inhibits neurogenesis in the adult dentate gyrus by down-regulating cyclic AMP response element binding protein phosphorylation. Neuroscience 141:827–836CrossRefPubMed

Titel: Role of inducible or neuronal nitric oxide synthase in neurogenesis of the dentate gyrus in aged mice
verfasst von: Minami Adachi
Manami Abe
Taeko Sasaki
Hiroyuki Kato
Jiro Kasahara
Tsutomu Araki
Publikationsdatum: 01.12.2010
Verlag: Springer US
Erschienen in: Metabolic Brain Disease / Ausgabe 4/2010
Print ISSN: 0885-7490
Elektronische ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-010-9224-8

Leitlinien kompakt für die Neurologie

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Neurologie

Hirnblutung unter DOAK und VKA ähnlich bedrohlich

17.05.2024 Direkte orale Antikoagulanzien Nachrichten

Kommt es zu einer nichttraumatischen Hirnblutung, spielt es keine große Rolle, ob die Betroffenen zuvor direkt wirksame orale Antikoagulanzien oder Marcumar bekommen haben: Die Prognose ist ähnlich schlecht.

Thrombektomie auch bei großen Infarkten von Vorteil

16.05.2024 Ischämischer Schlaganfall Nachrichten

Auch ein sehr ausgedehnter ischämischer Schlaganfall scheint an sich kein Grund zu sein, von einer mechanischen Thrombektomie abzusehen. Dafür spricht die LASTE-Studie, an der Patienten und Patientinnen mit einem ASPECTS von maximal 5 beteiligt waren.

Schwindelursache: Massagepistole lässt Otholiten tanzen

14.05.2024 Benigner Lagerungsschwindel Nachrichten

Wenn jüngere Menschen über ständig rezidivierenden Lagerungsschwindel klagen, könnte eine Massagepistole der Auslöser sein. In JAMA Otolaryngology warnt ein Team vor der Anwendung hochpotenter Geräte im Bereich des Nackens.

Schützt Olivenöl vor dem Tod durch Demenz?

10.05.2024 Morbus Alzheimer Nachrichten

Konsumieren Menschen täglich 7 Gramm Olivenöl, ist ihr Risiko, an einer Demenz zu sterben, um mehr als ein Viertel reduziert – und dies weitgehend unabhängig von ihrer sonstigen Ernährung. Dafür sprechen Auswertungen zweier großer US-Studien.

Update Neurologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 4/2010

Brain energy metabolism parameters in an animal model of diabetes

Editorial comment

The influence of L-carnitine suplementation on the antioxidative abilities of serum and the central nervous system of ethanol-induced rats

Glutamine synthetase gene expression and glutamate transporters in C6-glioma cells

Changes in regional brain volumes in social anxiety disorder following 12 weeks of treatment with escitalopram

Elevated cerebral glutamate and myo-inositol levels in cognitively normal middle-aged adults with metabolic syndrome