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Journal of Clinical Immunology

Erschienen in:

01.09.2009

Sequential Changes in Serum Cytokines Reflect Viral RNA Kinetics in Target Organs of a Coxsackievirus B Infection in Mice

verfasst von: Magnus Lundgren, Per Ola Darnerud, Jonas Blomberg, Göran Friman, Nils-Gunnar Ilbäck

Erschienen in: Journal of Clinical Immunology | Ausgabe 5/2009

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Abstract

Introduction

The pattern of cytokine responses related to viral replication during the course of the common human coxsackievirus B3 (CVB3) infection is not known.

Methods

Serum levels of 21 cytokines and chemokines were studied (Luminex technique) in CVB3-infected in mice on days 3, 6, and 9 post-infection (p.i.). CVB3 was measured quantitatively (reverse transcriptase polymerase chain reaction) in the liver and pancreas.

Results

Virus levels peaked on day 3 in both the liver and pancreas, but were 1,000-fold higher in the pancreas. IL-17α, IFN-γ, KC, MCP-1, MIP1β, and RANTES were detected on all days. On day 3 p.i., IL-6, IL-12(p40), KC, MCP-1, RANTES, and TNF-α were found to peak. On day 6 p.i., IL-1β, IL-9, IL-12(p70), IL-13, IL-17α, and IFN-γ peaked. On day 9 p.i., MIP1β, IL-1β, MCP-1, and TNF-α were still increased. These changes in cytokines may be used to monitor the progress of enteroviral infections in clinical settings.

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Titel: Sequential Changes in Serum Cytokines Reflect Viral RNA Kinetics in Target Organs of a Coxsackievirus B Infection in Mice
verfasst von: Magnus Lundgren
Per Ola Darnerud
Jonas Blomberg
Göran Friman
Nils-Gunnar Ilbäck
Publikationsdatum: 01.09.2009
Verlag: Springer US
Erschienen in: Journal of Clinical Immunology / Ausgabe 5/2009
Print ISSN: 0271-9142
Elektronische ISSN: 1573-2592
DOI: https://doi.org/10.1007/s10875-009-9294-8

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Abstract

Introduction

Methods

Results

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