Introduction
Materials and methods
Cases
Neuropathologic analysis
Sampling and routine and immunohistochemical staining
Analysis of the BNM
Quantitative analysis of phosphorylated tau immunoreactive (ptau+) neurons in the BNM
Semi-quantitative analysis of pTDP43 immunoreactive (pTDP43+) neurons in the BNM
Immunoblot analysis
Statistical analysis
Results
Clinical and pathologic studies
Infarct cases | Hemorrhage cases | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
case | side | Braak stage | interval (days) | age (y.o.) | sex | case | side | Braak stage | interval (days) | age (y.o.) | sex |
MCA territory
|
putamen
| ||||||||||
1 | R | 1 | 177 | 74 | M | 24 | R | 1 | 967 | 82 | M |
2 | L | 1 | 606 | 74 | M | 25 | R | 1 | 2720 | 58 | M |
3 | R | 1 | 1787 | 84 | M | 26 | L | 1 | 5017 | 81 | M |
4 | L | 1 | 2790 | 74 | M | 27 | R | 1 | 5955 | 83 | M |
5 | L | 1 | 3511 | 75 | M | 28 | L | 1 | 10,415 | 89 | M |
6 | R | 1 | 3975 | 85 | M | 29 | R | 2 | 2922 | 65 | M |
7 | R | 1 | 4821 | 71 | M | 30 | R | 2 | 5844 | 95 | F |
8 | R | 1 | 5114 | 92 | M | 31 | R | 3 | 1592 | 84 | M |
9 | L | 1 | 5114 | 77 | M | 32 | L | 3 | 2526 | 88 | M |
10 | L | 1 | 9869 | 87 | M | 33 | L | 4 | 4383 | 81 | F |
11 | L | 2 | 119 | 74 | M | 34 | L | 5 | 16,048 | 92 | F |
12 | R | 2 | 1707 | 83 | M | 35 | R | 6 | 4749 | 85 | F |
13 | R | 2 | 3525 | 82 | F | ||||||
14 | R | 2 | 7305 | 90 | M |
Frontal lobe
| |||||
15 | L | 2 | 7345 | 79 | M | 36 | L | 5 | 2376 | 82 | F |
16 | R | 3 | 4502 | 82 | M | ||||||
17 | L | 4 | 344 | 102 | F |
Occipital lobe
| |||||
18 | L | 4 | 730 | 91 | F | 37 | L | 1 | 7305 | 79 | M |
19 | L | 5 | 3168 | 86 | F | 38 | L | 2 | 3288 | 88 | M |
ACA territory
| |||||||||||
20 | R | 2 | 3891 | 75 | M |
Cerebellum
| |||||
PCA territory
| 39 | L | 2 | 1820 | 86 | M | |||||
21 | L | 1 | 1144 | 81 | M | ||||||
22 | R | 1 | 4564 | 87 | F |
Brain stem
| |||||
23 | L | 2 | 5516 | 83 | F | 40 | L | 1 | 529 | 69 | M |
NFTs of the BNM in CVD cases
-
Putaminal hemorrhage cases (11/12, 92%): The rate of ptau+ neurons was higher on the BNM-affected side than on the BNM-unaffected side (Fig. 2c). The median rate was significantly higher on the BNM-affected side than on the BNM-unaffected side (p < 0.01).
-
Putaminal hemorrhage cases (11/11, 100%): The rate of GB+ neurons was higher on the BNM-affected side than on the BNM-unaffected side (Fig. 2d). The median rate was significantly higher on the BNM-affected side than on the BNM-unaffected side (p < 0.01).
-
Other CVD cases: There were no significant differences in the number of ptau+ or GB+ BNM neurons between the BNM-affected and BNM-unaffected sides (Fig. 2e, f).