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Forensic Science, Medicine and Pathology

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03.07.2018 | Review

The effectiveness of decontamination procedures used in forensic hair analysis

verfasst von: Dylan Mantinieks, Dimitri Gerostamoulos, Paul Wright, Olaf Drummer

Erschienen in: Forensic Science, Medicine and Pathology | Ausgabe 3/2018

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Abstract

Hair is a mainstream specimen used in forensic toxicology to determine drug use and exposure. However, the interpretation of an analytical hair result can be complicated by the presence of external drug contamination. Decontamination procedures are included in hair analysis methods to remove external contamination, but the capacity of these washes to completely remove contamination for all drugs is controversial. It is evident that there is no consensus on the most effective decontamination procedure, nor can decontamination procedures consistently remove external drug contamination to less than reportable cut-offs for all analytes. ∆⁹-tetrahydrocannabinol deposited from cannabis smoke is mostly removed by organic solvents, whereas ionizable drugs are more effectively removed by an aqueous wash. Organizations such as the Society of Hair Testing recommend a hair decontamination procedure should include both an organic and aqueous washing step, which is in accordance with the reviewed literature. Studies involving a systematic evaluation of various solvents have shown that the most effective organic solvent was methanol and the most effective aqueous solvent contained sodium dodecyl sulfate detergent. If future systematic studies can demonstrate similar findings, a consensus on the most effective decontamination procedure for forensic hair analysis may be established.

Baumgartner AM, Jones PF, Baumgartner WA, Black CT. Radioimmunoassay of hair for determining opiate-abuse histories. J Nucl Med. 1979;20:748–52.PubMed

Cooper GA. Hair testing is taking root. Ann Clin Biochem. 2011;48:516–30.CrossRefPubMed

Huestis MA. Judicial acceptance of hair tests for substances of abuse in the United States courts: scientific, forensic, and ethical aspects. Ther Drug Monit. 1996;18:456–9.CrossRefPubMed

Xiang P, Shen M, Drummer OH. Review: drug concentrations in hair and their relevance in drug facilitated crimes. J Forensic Legal Med. 2015;36:126–35.CrossRef

Kintz P. Value of hair analysis in postmortem toxicology. Forensic Sci Int. 2004;142:127–34.CrossRefPubMed

Pragst F, Balikova MA. State of the art in hair analysis for detection of drug and alcohol abuse. Clin Chim Acta. 2006;370:17–49.CrossRefPubMed

Musshoff F, Driever F, Lachenmeier K, Lachenmeier DW, Banger M, Madea B. Results of hair analyses for drugs of abuse and comparison with self-reports and urine tests. Forensic Sci Int. 2006;156:118–23.CrossRefPubMed

Cooper GA, Kronstrand R, Kintz P. Society of Hair Testing guidelines for drug testing in hair. Forensic Sci Int. 2012;218:20–4.CrossRefPubMed

Henderson GL, Harkey MR, Jones R. "Hair analysis for drugs of abuse" final report on grant number NIJ 90-NIJ-CX-0012 to the National Institute of Justice and National Institute on Drug Abuse. 1993.

10.

Paterson S, Lee S, Cordero R. Analysis of hair after contamination with blood containing cocaine and blood containing benzoylecgonine. Forensic Sci Int. 2010;194:94–6.CrossRefPubMed

11.

Lee S, Han E, In S, Choi H, Chung H, Chung KH. Analysis of pubic hair as an alternative specimen to scalp hair: a contamination issue. Forensic Sci Int. 2011;206:19–21.CrossRefPubMed

12.

Duvivier WF, Peeters RJ, van Beek TA, Nielen MW. Evidence based decontamination protocols for the removal of external Δ9-tetrahydrocannabinol (THC) from contaminated hair. Forensic Sci Int. 2016;259:110–8.CrossRefPubMed

13.

Wang WL, Cone EJ. Testing human hair for drugs of abuse. IV. Environmental cocaine contamination and washing effects. Forensic Sci Int. 1995;70:39–51.CrossRefPubMed

14.

Romano G, Barbera N, Lombardo I. Hair testing for drugs of abuse: evaluation of external cocaine contamination and risk of false positives. Forensic Sci Int. 2001;123:119–29.CrossRefPubMed

15.

Wang X, Drummer OH. Review: interpretation of drug presence in the hair of children. Forensic Sci Int. 2015;257:458–72.CrossRefPubMed

16.

Wright J, Kenneally ME, Edwards JW, Walker GS. Adverse health effects associated with living in a former methamphetamine drug laboratory - Victoria, Australia, 2015. MMWR Morb Mortal Wkly Rep. 2017;65:1470–3.CrossRefPubMed

17.

Doran GS, Deans R, De Filippis C, Kostakis C, Howitt JA. The presence of licit and illicit drugs in police stations and their implications for workplace drug testing. Forensic Sci Int. 2017;278:125–36.CrossRefPubMed

18.

United Nations Office on Drugs and Crime. The analysis of drugs in hair. Guidelines for testing drugs under international control in hair, sweat and oral fluid. Vienna: United Nations; 2014. p. 7–40.

19.

Kintz P. A novel approach to document single exposure to GHB: hair analysis after sweat contamination. J Anal Toxicol. 2016;40:563–4.CrossRefPubMed

20.

Thompson v. Civil Serv. Commn., Mass. App. Ct. 15, 330. 2016.

21.

Goldberger BA, Caplan YH, Maguire T, Cone EJ. Testing human hair for drugs of abuse. III. Identification of heroin and 6-acetylmorphine as indicators of heroin use. J Anal Toxicol. 1991;15:226–31.CrossRefPubMed

22.

Cone EJ, Joseph RE. The potential for bias in hair testing for drugs of abuse. In: Kintz P, editor. Drug testing in hair. Boca Raton: CRC Press; 1996. p. 69–93.

23.

Kidwell DA, Lee EH, Delauder SF. Evidence for bias in hair testing and procedures to correct bias. Forensic Sci Int. 2000;107:39–61.CrossRefPubMed

24.

DeLauder SF, Kidwell DA. The incorporation of dyes into hair as a model for drug binding. Forensic Sci Int. 2000;107:93–104.CrossRefPubMed

25.

Testorf MF, Kronstrand R, Svensson SP, Lundstrom I, Ahlner J. Characterization of [3H]flunitrazepam binding to melanin. Anal Biochem. 2001;298:259–64.CrossRefPubMed

26.

Kintz P. Value of the concept of minimal detectable dosage in human hair. Forensic Sci Int. 2012;218:28–30.CrossRefPubMed

27.

Nakahara Y, Takahashi K, Kikura R. Hair analysis for drugs of abuse. X. Effect of physicochemical properties of drugs on the incorporation rates into hair. Biol Pharm Bull. 1995;18:1223–7.CrossRefPubMed

28.

Tsanaclis L, Wicks JFC. Differentiation between drug use and environmental contamination when testing for drugs in hair. Forensic Sci Int. 2008;176:19–22.CrossRefPubMed

29.

Kintz P. Segmental hair analysis can demonstrate external contamination in postmortem cases. Forensic Sci Int. 2011;215:73–6.CrossRefPubMed

30.

Moosmann B, Roth N, Auwarter V. Hair analysis for Δ9-tetrahydrocannabinolic acid a (THCA-A) and Δ9-tetrahydrocannabinol (THC) after handling cannabis plant material. Drug Test Anal. 2016;8:128–32.CrossRefPubMed

31.

Bush DM. The U.S. mandatory guidelines for federal workplace drug testing programs: current status and future considerations. Forensic Sci Int. 2008;174:111–9.CrossRefPubMed

32.

Agius R, Kintz P. Guidelines for European workplace drug and alcohol testing in hair. Drug Test Anal. 2010;2:367–76.CrossRefPubMed

33.

White RM. Drugs in hair. Part I. Metabolisms of major drug classes. Forensic Sci Rev. 2017;29:23–55.PubMed

34.

Schaffer M, Cheng CC, Chao O, Hill V, Matsui P. Analysis of cocaine and metabolites in hair: validation and application of measurement of hydroxycocaine metabolites as evidence of cocaine ingestion. Anal Bioanal Chem. 2016;408:2043–54.CrossRefPubMed

35.

Morris-Kukoski CL, Montgomery MA, Hammer RL. Analysis of extensively washed hair from cocaine users and drug chemists to establish new reporting criteria. J Anal Toxicol. 2014;38:628–36.CrossRefPubMed

36.

Pichini S, Marchei E, Martello S, Gottardi M, Pellegrini M, Svaizer F, et al. Identification and quantification of 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid glucuronide (THC-COOH-glu) in hair by ultra-performance liquid chromatography tandem mass spectrometry as a potential hair biomarker of cannabis use. Forensic Sci Int. 2015;249:47–51.CrossRefPubMed

37.

Gambelunghe C, Rossi R, Aroni K, Gili A, Bacci M, Pascali V, et al. Norcocaine and cocaethylene distribution patterns in hair samples from light, moderate, and heavy cocaine users. Drug Test Anal. 2017;9:161–7.CrossRefPubMed

38.

Madry MM, Rust KY, Guglielmello R, Baumgartner MR, Kraemer T. Metabolite to parent drug concentration ratios in hair for the differentiation of tramadol intake from external contamination and passive exposure. Forensic Sci Int. 2012;223:330–4.CrossRefPubMed

39.

Blank DL, Kidwell DA. Decontamination procedures for drugs of abuse in hair: are they sufficient? Forensic Sci Int. 1995;70:13–38.CrossRefPubMed

40.

Ropero-Miller JD, Huestis MA, Stout PR. Cocaine analytes in human hair: evaluation of concentration ratios in different cocaine sources, drug-user populations and surface-contaminated specimens. J Anal Toxicol. 2012;36:390–8.CrossRefPubMed

41.

Auwärter V, Wohlfarth A, Traber J, Thieme D, Weinmann W. Hair analysis for Δ9-tetrahydrocannabinolic acid A—new insights into the mechanism of drug incorporation of cannabinoids into hair. Forensic Sci Int. 2010;196:10–3.CrossRefPubMed

42.

Moosmann B, Roth N, Hastedt M, Jacobsen-Bauer A, Pragst F, Auwärter V. Cannabinoid findings in children hair – what do they really tell us? An assessment in the light of three different analytical methods with focus on interpretation of Δ9-tetrahydrocannabinolic acid a concentrations. Drug Test Anal. 2015;7:349–57.CrossRefPubMed

43.

Kintz P, Evans J, Villain M, Cirimele V. Interpretation of hair findings in children after methadone poisoning. Forensic Sci Int. 2010;196:51–4.CrossRefPubMed

44.

Romano G, Barbera N, Spadaro G, Valenti V. Determination of drugs of abuse in hair: evaluation of external heroin contamination and risk of false positives. Forensic Sci Int. 2003;131:98–102.CrossRefPubMed

45.

Thorspecken J, Skopp G, Potsch L. In vitro contamination of hair by marijuana smoke. Clin Chem. 2004;50:596–602.CrossRefPubMed

46.

Poetzsch M, Baumgartner MR, Steuer AE, Kraemer T. Segmental hair analysis for differentiation of tilidine intake from external contamination using LC-ESI-MS/MS and MALDI-MS/MS imaging. Drug Test Anal. 2015;7:143–9.CrossRefPubMed

47.

Baumgartner WA, Hill VA. Sample preparation techniques. Forensic Sci Int. 1993;63:121–35.CrossRefPubMed

48.

Potsch L, Moeller MR. On pathways for small molecules into and out of human hair fibers. J Forensic Sci. 1996;41:121–5.CrossRefPubMed

49.

Cuypers E, Flinders B, Boone CM, Bosman IJ, Lusthof KJ, Van Asten AC, et al. Consequences of decontamination procedures in forensic hair analysis using metal-assisted secondary ion mass spectrometry analysis. Anal Chem. 2016;88:3091–7.CrossRefPubMed

50.

Paulsen RB, Wilkins DG, Slawson MH, Shaw K, Rollins DE. Effect of four laboratory decontamination procedures on the quantitative determination of cocaine and metabolites in hair by HPLC-MS. J Anal Toxicol. 2001;25:490–6.CrossRefPubMed

51.

Hill VA, Loni E, Cairns T, Sommer J, Schaffer M. Identification and analysis of damaged or porous hair. Drug Test Anal. 2014;6:42–54.CrossRefPubMed

52.

Schaffer M, Hill VA, Cairns T. Hair analysis for cocaine: the requirement for effective wash procedures and effects of drug concentration and hair porosity in contamination and decontamination. J Anal Toxicol. 2005;29:319–26.CrossRefPubMed

53.

Cairns T, Hill VA, Schaffer M, Thistle W. Removing and identifying drug contamination in the analysis of human hair. Forensic Sci Int. 2004;145:97–108.CrossRefPubMed

54.

Schaffer M, Wang WL, Irving J. An evaluation of two wash procedures for the differentiation of external contamination versus ingestion in the analysis of human hair samples for cocaine. J Anal Toxicol. 2002;26:485–8.CrossRefPubMed

55.

Paterson S, Lee S, Cordero R. Analysis of hair after contamination with blood containing 6-acetylmorphine and blood containing morphine. Forensic Sci Int. 2011;210:129–32.CrossRefPubMed

56.

Kidwell DA, Smith FP. Passive exposure, decontamination procedures, cutoffs, and bias. In: Kintz P, editor. Analytical and practical aspects of drug testing in hair. Boca Raton: CRC Press; 2006. p. 25–72.CrossRef

57.

Bassindale T. Quantitative analysis of methamphetamine in hair of children removed from clandestine laboratories - evidence of passive exposure? Forensic Sci Int. 2012;219:179–82.CrossRefPubMed

58.

Kidwell DA, Smith FP. Companies should test the limits of their decontamination procedures--reply to: comments on "ethnic hair care products may increase false positive in hair" V. Hill et al. Forensic Sci Int. 2016;259:e51.CrossRefPubMed

59.

Moosmann B, Roth N, Auwarter V. Hair analysis for THCA-A, THC and CBN after passive in vivo exposure to marijuana smoke. Drug Test Anal. 2014;6:119–25.CrossRefPubMed

60.

Cone EJ, Yousefnejad D, Darwin WD, Maguire T. Testing human hair for drugs of abuse. II. Identification of unique cocaine metabolites in hair of drug abusers and evaluation of decontamination procedures. J Anal Toxicol. 1991;15:250–5.CrossRefPubMed

61.

Duca RC, Hardy E, Salquebre G, Appenzeller BM. Hair decontamination procedure prior to multi-class pesticide analysis. Drug Test Anal. 2014;6:55–66.CrossRefPubMed

62.

Hill VA, Cairns T, Schaffer M. Hair analysis for cocaine: Factors in laboratory contamination studies and their relevance to proficiency sample preparation and hair testing practices. Forensic Sci Int. 2008;176(1):23–33.

63.

Stout PR, Ropero-Miller JD, Baylor MR, Mitchell JM. External contamination of hair with cocaine: Evaluation of external cocaine contamination and development of performance-testing materials. J Anal Toxicol. 2006;30(8):490–500.

64.

Romano G, Indorato F, Spadaro G, Barbera S, Barbera N. Cocaine contamination in pubic hair: Analysis of the decontamination method. Egypt J Forensic Sci. 2014;4(4):129–36.

65.

Kidwell DA, Smith FP, Shepherd AR. Ethnic hair care products may increase false positives in hair drug testing. Forensic Sci Int. 2015;257:160–4.

Titel: The effectiveness of decontamination procedures used in forensic hair analysis
verfasst von: Dylan Mantinieks
Dimitri Gerostamoulos
Paul Wright
Olaf Drummer
Publikationsdatum: 03.07.2018
Verlag: Springer US
Erschienen in: Forensic Science, Medicine and Pathology / Ausgabe 3/2018
Print ISSN: 1547-769X
Elektronische ISSN: 1556-2891
DOI: https://doi.org/10.1007/s12024-018-9994-6

Springer Medizin

The effectiveness of decontamination procedures used in forensic hair analysis

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

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