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International Journal of Colorectal Disease

Erschienen in:

01.02.2013 | Original Article

The role of colonic mast cells and myenteric plexitis in patients with diverticular disease

verfasst von: Gabrio Bassotti, Vincenzo Villanacci, Riccardo Nascimbeni, Elisabetta Antonelli, Moris Cadei, Stefania Manenti, Luisa Lorenzi, Amin Titi, Bruno Salerni

Erschienen in: International Journal of Colorectal Disease | Ausgabe 2/2013

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Abstract

Background

Gut mast cells represent an important cell population involved in intestinal homeostasis and inflammatory processes. However, their possible role has not to date been investigated in colonic diverticular disease.

Aims

This study aims to evaluate colonic mast cells in patients undergoing surgery for diverticular disease.

Methods

Surgical resection samples from 27 patients undergoing surgery for diverticular disease (12 emergency procedures for severe disease and 15 elective procedures) were evaluated. The number of mast cells was assessed in the various layers by means of a specific antibody (tryptase) and compared with those evaluated in ten controls. In patients with mast cells degranulation, double immunohistochemistry, also assessing nerve fibres, was carried out. In addition, the presence of myenteric plexitis was sought.

Results

Compared with controls, the number of mast cells in diverticular patients was significantly increased, both as an overall figure and in the various layers of the large bowel. In patients in whom mast cells degranulation was present, these were always closed to nerve fibres. No differences were found between the two subgroups of patients with respect to the number and distribution of mast cells; however, all patients undergoing emergency surgery (but none of those undergoing elective procedures) had myenteric plexitis, represented by lymphocytic infiltration in 67 % and eosinophilic infiltration in 33 % of cases.

Conclusions

Patients with diverticular disease display an increase of mast cells in the large bowel. The presence of myenteric plexitis in those with complicated, severe disease, suggest that this could represent a histopathologic marker of more aggressive disease.

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Titel: The role of colonic mast cells and myenteric plexitis in patients with diverticular disease
verfasst von: Gabrio Bassotti
Vincenzo Villanacci
Riccardo Nascimbeni
Elisabetta Antonelli
Moris Cadei
Stefania Manenti
Luisa Lorenzi
Amin Titi
Bruno Salerni
Publikationsdatum: 01.02.2013
Verlag: Springer-Verlag
Erschienen in: International Journal of Colorectal Disease / Ausgabe 2/2013
Print ISSN: 0179-1958
Elektronische ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-012-1554-z

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Abstract

Background

Aims

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Results

Conclusions

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