Background
Exosomes and tumor-derived exosomes
Contents | Methods | References |
---|---|---|
Isolation and purification | Ultracentrifugation Density gradients centrifugation Immunobeads Size exclusion chromatography Spin column-based methods | |
Analysis of size and shape | TEM | [132] |
Analysis of size distribution and concentration | Nanoparticle Tracking Analysis FAVS | |
Analysis of proteins | Mass spectrometry, Western-blot SDS-PAGE | |
Analysis of RNA | NGS, Microarray, RT-PCR |
Roles of Tumor-derived exosomes in the NSCLC
Tumor-derived exosomes in the growth and progression of NSCLC
Tumor-derived exosomes in the invasion and metastasis of NSCLC
Contents | Findings | References |
---|---|---|
Invasion | exosomal TGF-β and IL-10 may enhance migration ability in vitro under hypoxia | [47] |
exosomal vimentin may induce EMT | [51] | |
exosomal Rab3D which activates AKT/GSK3β signaling may induce EMT | [52] | |
exosomal miR-23a may affect the changes of EMT related phenotype | [53] | |
Exosomal miR-302b can inhibit the proliferation and migration of lung cancer cells through the TGFβRII/ERK pathway | [54] | |
Drug resistance | exosomal VEGF and TGF2β may result in drug resistance | |
exosomal proteins and phospholipids components are involved in gefitinib resistance | ||
exosomes may antagonize the chemotherapeutic effect of cisplatin by upregulating autophagy | [65] | |
exosomes can regulate the combination of antibody targeting drugs and tumor cells |
Tumor-derived exosomes in the targeted drug resistance
Tumor-derived exosomes as markers in prognosis of NSCLC
Clinical implications of tumor-derived exosomes in the targeted therapy of NSCLC
Tumor-derived exosomes as biomarkers for NSCLC diagnosis and targeted therapy guidance
Tumor-derived exosomes as biomarkers for NSCLC targeted therapy resistance
The potential value of tumor-derived exosomes in the targeted therapy of NSCLC
Potential value | Methods | References |
---|---|---|
A tool for drug delivery | Use electroporation or lipofection | |
A direct target for reducing the content | Blood purification: ADAPT, hollow fiber filtration technology Change the local pH of the tumor: PPIs Drug usage: GW4869, amiloride, diannexin Interfere with signal pathway: knockout Rab27, SYTL4 ,EXPH5, ISGylation and syndecan-syntenin-ALIX | |
Other targets | let-7, LKB1, CD9, CD63, surviving, miR-302b |